Equipe PFID

Dr Alexandra Holland

Areas of interest/expertise:
  • Molecular biology.

  • PhD in Chemical Engineering (Dec 2007) University of Washington, WA, USA 2001-2007.
  • BS Chemical Engineering with Honors, UC Berkeley, CA, USA 1997-2001.
  • French Scientific Baccalaureate Lycée Henri IV, Paris, France 1995.

Honors and Fellowships
  • Foundation Fourmentin Fellow with Prof. Danchin at the Pasteur Institute 2009
  • NSF International MCCE IGERT Fellow at the U. of Washington 2006-2007
  • NSF GRFP (Graduate Research Fellowship Program) Fellow at the U. of Washington 2002-2005
  • Achievement Reward for Research Scientist Fellow at the U. of Washington 2001-2004
  • UC Berkeley President's Undergraduate Fellowship (funding for independent research) 2000-2001
  • American Institute of Chemical Engineers UC Berkeley Outstanding Senior Award 2001
  • Dow Chemical UC Berkeley Outstanding Junior Award 2000

Post-Doctoral Experiences
  • Visiting Scholar at the University of Manchester, UK (with Prof. Sigee) Feb-July 2008
    Micro-algae physiological studies and modeling to determine intrinsic productivity
    Novel parametrization of algae bioreactors suitable for controller design
    Results filed for a non-provisional patent with the SEED IP law firm in Seattle, WA.
  • Visiting Scientist at the University of Washington, USA (with Prof. Lidstrom) Aug-Dec 2008
    Further genetic and physiological studies of Deinococcus radiodurans to refine doctorate hypotheses. The proposed mechanisms, consisting of three novel regulatory interactions involving overlapping phosphate regulation and polyphosphate metabolism, may be widespread in bacteria.
  • Post-Doctorate at the Pasteur Institute, Paris, France (with Prof. Danchin) Feb-May 2009
    Enzymatic polyphosphate assay. Evidence that phoU deletion leads to elevated polyphosphate levels in the Gram positive Streptomyces lividans (collaboration with Dr Marie-Joëlle Virolle).
  • Unaffiliated Research in collaboration with Dan Zelazo, UW Aeronautics & Astronautics Jun-Sep 2009
    Preparation of a Letter to the Editor of PLOS Biology critiquing two articles in the edition of January 2009. This critique addresses the misuse of Control Engineering principles and terminology in Biology, which extends much beyond these publications, and reviews recent promising developments in different fields of Control Theory.
  • Post-Doctorate at Brigham Young University, Provo, Utah (with WR McCleary) Oct 2009-Dec 2010
    Elucidation of the regulatory interactions between phosphate metabolic regulation and polyphosphate metabolism.
  • Post-Doctorate at Purdue University, West Lafayette, IN (with Barry Wanner) Feb 2011-Jun 2011
    Comparative transcriptional profiling (RNA-Seq) of Deinococcus radiodurans and E. coli under phosphate limitation. Polyphosphate metabolism.
  • Post-Doctorate at the Pasteur Institute, Paris, France (with Spencer Shorte) Oct 2011-present
    Development of in vitro applications for the Fluorescence by Unbound Excitation from Luminescence (FUEL, patent pending) phenomenon. Use of FUEL to evidence protein interactions in vitro and enhance signal resolution in vivo.

Research publications
  • Holland AD, Rothfuss HM and ME Lidstrom. Development of a defined medium supporting rapid growth for Deinococcus radiodurans and analysis of metabolic capacities. Applied Microbiology and Biotechnology. 2006 72 (5): 1074-82
  • Holland AD, Dragavon J and DC Sigee. Methods for Estimating Intrinsic Autotrophic Biomass Yield and Productivity in Algae: Emphasis on Experimental Methods for Strain Selection. Biotechnology Journal. 2011 6:572-583
  • Holland AD and D Wheeler. Methods for Estimating Intrinsic Autotrophic Biomass Yield and Productivity in Algae: Modeling Spectrum and Mixing-Rate Dependence. Accepted for publication in the Biotechnology Journal. 2011 6:584-599
  • Holland AD and J Dragavon. Algal Reactor Design Based on Comprehensive Modeling of Light and Mixing. In Bajpai RK, Prokop Ales and Mark Zappl (Ed.) Algal Biorefineries, vol.1. Springer Publishing, New York, USA. (in press).