Biochimie des Interactions Macromoléculaires - Projet Alexandre Chenal

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Publications (reviews and application notes are listed below)


Giganti D, Albesa-Jové D, Urresti S, Rodrigo-Unzueta A, Martínez MA, Comino N, Barilone N, Bellinzoni M, Chenal A, Guerin ME, Alzari PM. (2014). Secondary structure reshuffling modulates glycosyltransferase function at the membrane. Nat Chem Biol. 2014 Nov 17. doi: 10.1038/nchembio.1694. (pdf)

Karst JC, Ntsogo Enguéné VY, Cannella SE, Subrini O, Hessel A, Debard S, Ladant D, Chenal A.(2014). Calcium, Acylation, and Molecular Confinement Favor Folding of Bordetella pertussis Adenylate Cyclase CyaA Toxin into a Monomeric and Cytotoxic Form. J Biol Chem. 2014 Oct 31;289(44):30702-16. (pdf)

Selwa E, Davi M, Chenal A, Sotomayor-Pérez AC, Ladant D, Malliavin TE. (2014). Allosteric Activation of Bordetella pertussis Adenylyl Cyclase by Calmodulin: MOLECULAR DYNAMICS AND MUTAGENESIS STUDIES. J Biol Chem. 2014 Jul 25;289(30):21131-21141. (pdf)


Veneziano R, Rossi C, Chenal A, Devoisselle JM, Ladant D, Chopineau J. (2013). Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer. Proc Natl Acad Sci U S A. 2013 Dec 2. (pdf)

Subrini O, Sotomayor Perez AC, Hessel A, Spiaczka-Karst J, Selwa E, Sapay N, Veneziano R, Pansieri J, Chopineau J, Ladant D, Chenal A. (2013). Characterization of a Membrane-Active Peptide from the Bordetella pertussis CyaA Toxin. J Biol Chem. 2013 Nov 8;288(45):32585-98. doi: 10.1074/jbc.M113.508838. (pdf)

Toxin Gets Support From A Crowd. J. Am. Chem. Soc. Spotlight, 2013, 135 (35), p 12923, DOI: 10.1021/ja408820k, September 4, 2013 (pdf)

Sotomayor-Pérez AC, Subrini O, Hessel A, Ladant D, Chenal A. (2013). Molecular Crowding Stabilizes Both the Intrinsically Disordered Calcium-Free State and the Folded Calcium-Bound State of a Repeat in Toxin (RTX) Protein. J Am Chem Soc. 2013 Aug 14;135(32):11929-34. doi: 10.1021/ja404790f. (pdf)   (F1000 prime)


Karst, J.C., Barker R., Devi U., Swann M.J., Davi M., Roser S.J., Ladant D. & Chenal A. (2012). Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin. J Biol Chem 287, 9200-12. (pdf)

Chenal A, Vendrely C, Vitrac H, Karst J.C., Gonneau A., Blanchet C. E., Pichard S., Garcia E., Salin B., Catty C., Gillet D., Hussy N., Marquette C., Almunia C., and Forge V. (2012) Amyloid fibrils formed by the programmed  cell death regulator Bcl-xL. J Mol. Biol. Jan 20;415(3):584-99. (pdf)

Karst J.C., Sotomayor-Pérez A. C., Ladant D. and Chenal A. (2012)  Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods. Part D. Methods to assess protein size and shape, chapter 39, in Experimental tools for the intrinsically disordered protein analysis: Vol II, Humana Press series "Methods in Molecular Biology", Vladimir Uversky and A. Keith Dunker, Eds. Methods Mol Biol. 2012;896:163-77. (pdf)

Sotomayor-Pérez A. C., Karst J.C., Ladant D. and Chenal A. (2012)  Mean net charge of intrinsically disordered proteins: Experimental determination of protein valence by electrophoretic mobility measurements. Part E. Methods to analyse conformational behavior, chapter 51, in Experimental tools for the intrinsically disordered protein analysis: Vol II, Humana Press series "Methods in Molecular Biology", Vladimir Uversky and A. Keith Dunker, Eds. Methods Mol Biol. 2012;896:331-49. (pdf)


Sotomayor-Pérez A. C., Ladant D., Chenal A. (2011) Calcium-induced Folding of Intrinsically Disordered Repeat-in-Toxin (RTX) Motifs via Changes of Protein Charges and Oligomerization States. J Biol Chem. 13;286(19):16997-7004 (pdf) (sup mat)


Chenal, A., Karst, J. C., Perez, A. C., Wozniak, A. K., Baron, B., England, P., and Ladant, D. (2010) Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin, Biophys J 99, 3744-3753. (pdf) (sup mat)

Sotomayor Perez, A. C., Karst, J. C., Davi, M., Guijarro, J. I., Ladant, D., and Chenal, A. (2010) Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin, J Mol Biol 397, 534-549. (pdf) (sup mat)

Karst, J. C., Sotomayor Perez, A. C., Guijarro, J. I., Raynal, B., Chenal, A., and Ladant, D. (2010) Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin, Biochemistry 49, 318-328. (pdf)

Knapp, O., Maier, E., Mkaddem, S. B., Benz, R., Bens, M., Chenal, A., Geny, B., Vandewalle, A., and Popoff, M. R. (2010) Clostridium septicum alpha-toxin forms pores and induces rapid cell necrosis, Toxicon 55, 61-72. (pdf)


Chenal, A., Prongidi-Fix, L., Perier, A., Aisenbrey, C., Vernier, G., Lambotte, S., Haertlein, M., Dauvergne, M. T., Fragneto, G., Bechinger, B., Gillet, D., Forge, V., and Ferrand, M. (2009) Deciphering membrane insertion of the diphtheria toxin T domain by specular neutron reflectometry and solid-state NMR spectroscopy, J Mol Biol 391, 872-883. (pdf) (sup mat)

Aisenbrey, C., Prongidi-Fix, L., Chenal, A., Gillet, D., and Bechinger, B. (2009) Side chain resonances in static oriented proton-decoupled 15N solid-state NMR spectra of membrane proteins, J Am Chem Soc 131, 6340-6341. (pdf) (sup mat)

Bourdeau, R. W., Malito, E., Chenal, A., Bishop, B. L., Musch, M. W., Villereal, M. L., Chang, E. B., Mosser, E. M., Rest, R. F., and Tang, W. J. (2009) Cellular functions and X-ray structure of anthrolysin O, a cholesterol-dependent cytolysin secreted by Bacillus anthracis, J Biol Chem 284, 14645-14656. (pdf) (sup mat)

Chenal, A., Guijarro, J. I., Raynal, B., Delepierre, M., and Ladant, D. (2009) RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion, J Biol Chem 284, 1781-1789. (pdf)


Galloux, M., Vitrac, H., Montagner, C., Raffestin, S., Popoff, M. R., Chenal, A., Forge, V., and Gillet, D. (2008) Membrane Interaction of botulinum neurotoxin A translocation (T) domain. The belt region is a regulatory loop for membrane interaction, J Biol Chem 283, 27668-27676. (pdf)


Rohde, J. R., Breitkreutz, A., Chenal, A., Sansonetti, P. J., and Parsot, C. (2007) Type III secretion effectors of the IpaH family are E3 ubiquitin ligases, Cell Host Microbe 1, 77-83. (pdf) (sup mat)

Perier, A., Chassaing, A., Raffestin, S., Pichard, S., Masella, M., Menez, A., Forge, V., Chenal, A., and Gillet, D. (2007) Concerted protonation of key histidines triggers membrane interaction of the diphtheria toxin T domain, J Biol Chem 282, 24239-24245. (pdf) (sup mat)

Man, P., Montagner, C., Vernier, G., Dublet, B., Chenal, A., Forest, E., and Forge, V. (2007) Defining the interacting regions between apomyoglobin and lipid membrane by hydrogen/deuterium exchange coupled to mass spectrometry, J Mol Biol 368, 464-472. (pdf)

Montagner, C., Perier, A., Pichard, S., Vernier, G., Menez, A., Gillet, D., Forge, V., and Chenal, A. (2007) Behavior of the N-terminal helices of the diphtheria toxin T domain during the successive steps of membrane interaction, Biochemistry 46, 1878-1887. (pdf)

Vernier, G., Chenal, A., Vitrac, H., Barumandzadhe, R., Montagner, C., and Forge, V. (2007) Interactions of apomyoglobin with membranes: mechanisms and effects on heme uptake, Protein Sci 16, 391-400. (pdf)


Bauche, C., Chenal, A., Knapp, O., Bodenreider, C., Benz, R., Chaffotte, A., and Ladant, D. (2006) Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin, J Biol Chem 281, 16914-16926. (pdf)


Chenal, A., Vernier, G., Savarin, P., Bushmarina, N. A., Geze, A., Guillain, F., Gillet, D., and Forge, V. (2005) Conformational states and thermodynamics of alpha-lactalbumin bound to membranes: a case study of the effects of pH, calcium, lipid membrane curvature and charge, J Mol Biol 349, 890-905. (pdf)

Batard, T., Didierlaurent, A., Chabre, H., Mothes, N., Bussieres, L., Bohle, B., Couret, M. N., Ball, T., Lemoine, P., Focks Tejkl, M., Chenal, A., Clement, G., Dupont, F., Valent, P., Krauth, M. T., Andre, C., Valenta, R., and Moingeon, P. (2005) Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy, Int Arch Allergy Immunol 136, 239-249. (pdf)


Buhot, C., Chenal, A., Sanson, A., Pouvelle-Moratille, S., Gelb, M. H., Menez, A., Gillet, D., and Maillere, B. (2004) Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity, Protein Sci 13, 2970-2978. (pdf)

Tavallaie, M., Chenal, A., Gillet, D., Pereira, Y., Manich, M., Gibert, M., Raffestin, S., Popoff, M. R., and Marvaud, J. C. (2004) Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies, FEBS Lett 572, 299-306. (pdf)


Nizard, P., Gross, D. A., Babon, A., Chenal, A., Beaumelle, B., Kosmatopoulos, K., and Gillet, D. (2003) Anchoring cytokines to tumor cells for the preparation of anticancer vaccines without gene transfection in mice, J Immunother 26, 63-71. (pdf)


Marvaud, J. C., Stiles, B. G., Chenal, A., Gillet, D., Gibert, M., Smith, L. A., and Popoff, M. R. (2002) Clostridium perfringens iota toxin. Mapping of the Ia domain involved in docking with Ib and cellular internalization, J Biol Chem 277, 43659-43666. (pdf)

Chenal, A., Savarin, P., Nizard, P., Guillain, F., Gillet, D., and Forge, V. (2002) Membrane protein insertion regulated by bringing electrostatic and hydrophobic interactions into play. A case study with the translocation domain of diphtheria toxin, J Biol Chem 277, 43425-43432. (pdf)

Chenal, A., Nizard, P., Forge, V., Pugniere, M., Roy, M. O., Mani, J. C., Guillain, F., and Gillet, D. (2002) Does fusion of domains from unrelated proteins affect their folding pathways and the structural changes involved in their function? A case study with the diphtheria toxin T domain, Protein Eng 15, 383-391. (pdf)


Nizard, P., Gross, D. A., Chenal, A., Beaumelle, B., Kosmatopoulos, K., and Gillet, D. (2001) Anchoring cytokines to cancer cells using diphtheria toxin: better than immunotherapy by gene transfer? J Soc Biol 195, 229-234.

Nizard, P., Chenal, A., Beaumelle, B., Fourcade, A., and Gillet, D. (2001) Prolonged display or rapid internalization of the IgG-binding protein ZZ anchored to the surface of cells using the diphtheria toxin T domain, Protein Eng 14, 439-446. (pdf)

Reviews & Application Notes

Characterization of Protein-Protein Interactions. A Malvern Instrument application note, 2014. (pdf)

Overcoming the negative effect of protein structure on molecular weight measurement by Size Exclusion Chromatography. A Malvern Instrument application note, 2014. (pdf)

Characterization of hydrodynamic changes upon cation-binding to proteins. A Malvern Instrument application note, 2014. (pdf)

Chenal, A., B. Raynal, P. England and D. Ladant. Calcium-induced hydrodynamic changes of the RTX region of the adenylate cyclase toxin from Bordetella pertussis by SEC-TDA. Application Note from Viscotek, a Malvern company, 2009. (pdf)
Perier, A., A. Chenal, A. Babon, A. Ménez and D. Gillet. Engineering of bacterial toxins for research and medicine. In The comprehensive sourcebook of bacterial protein toxins (3rd edition), Joseph Alouf and Michel Popoff Eds, Elsevier, London, 2006, pp. 991-1007. (pdf)
Gillet, D., A. Chenal et A. Ménez. Ricine et risque biologique. In Menace terroriste nucléaire, radiologique, biologique, chimique – Approche médicale, M.C. de Revel, M.C.S. Gourmelon, P.C. Vidal, P.C.S. Renaudeau Eds., John Libbey Eurotext, Montrouge. pp. 266-272, 2005. (pdf)
Chenal, A., D. Gillet, J.M. Grognet et A. Ménez, Les principaux agents non vivants du risque biologique d'aujourd'hui. in Biofutur, vol. 248, pp. 28-32, 2004. (pdf)
Chenal, A., A. Babon, A. Perier, V. Forge and D. Gillet. From Biophysics to Engineering of the translocation domain of the diphtheria toxin. Recent Res. Devel. Biochem., in Research Signpost, Vol. 4, pp 985-1000, 2004. (pdf)
Chenal, A., P. Nizard, and D. Gillet, Structure and function of diphtheria toxin: from pathology to engineering. J. Tox.-Tox. Rev., 21: 321-359, 2002. (pdf)