F. Stavru et al. (2011). PNAS 108:9, 3612-3617
Mitochondrial fragmentation upon infection with Listeria monocytogenes.
(A) HeLa cells infected with L. monocytogenes (1 h; MOI of 50) display strongly fragmented mitochondria, whereas cells infected with L. innocua expressing InlB to enter cells do not. Mitochondria were stained with MitoTracker Deep Red (red). L. innocua or L. monocytogenes were stained with polyclonal antibodies R6 and R11 (green). Arrows indicate infected cells, and insets show 2× enlargements. (B) Infection (1 h, MOI of 50) with E.coli(Inv) as a model for Yersinia infection, Shigella flexneri (M09T), Salmonella enterica serovar typhimurium, and enteropathogenic E. coli (EPEC) do not cause mitochondrial fragmentation. Cell nuclei and bacteria were stained with DAPI (blue). Salmonella additionally expresses GFP (green). Mitochondria were stained as in A. Arrows indicate infected cells, and insets show 2× enlargements.