A. Lebreton et al. (2011). Science 331:1319
The BAHD1 complex represses ISGs in Listeria-infected epithelial cells.
Tandem affinity purification of the BAHD1-associated complex. Solubilized chromatin extracts from HEK293 cells expressing the HPT-BAHD1 fusion or control cells were first purified on anti–protein C affinity matrix, followed by polishing on nickel-Sepharose. Eluted fractions from the first (E1) and second (E2) affinity columns were analyzed by colloidal Coomassie staining (left) or immunoblot (right). I, input; FT, flow-through from first column. Histone H4 was a control for nonspecific binding of chromatin components.