Lecuit M. and Cossart P (2002), TRENDS in Molecular Medicine, vol 8 pp 537-542
A: Enterocyte-restricted human E-cadherin expression in iFABP-hEcad [human E-cadherin under the control of the promoter of the intestinal fatty-acid-binding protein (iFABP) gene] transgenic mice. Small intestine sections of a iFABP-hEcad transgenic mouse (left) and a non-transgenic littermate (right) immunolabeled with anti-hEcad HECD1 mouse monoclonal antibody (red). HECD1 recognizes human but not mouse E-cadherin. The background signal in the lamina propria is because of the reactivity of the secondary goat anti-mouse IgG antibody towards endogenous mouse IgG. A cross-section of a transgenic intestinal epithelial sheet allows detection of the typical honeycomb-like pattern of E-cadherin labeling. Scale BAR = 10 μm.B: Listeria monocytogenes multiplication within the lamina propria of a small-intestinal villus of an iFABP-hEcad transgenic mouse 48 h after intragastric inoculation. A small intestine section of a iFABP-hEcad transgenic mouse orally infected with L. monocytogenes and immunolabeled with rabbit anti-L. monocytogenes R11 polyclonal antibody (red). L. monocytogenes have crossed the intestinal barrier and replicate in the lamina propria, a phenomenon that is never observed in non-transgenic mice. Scale BAR = 10 μm.