Images

Figure from Pizarro-Cerda et al. Gram Positive Pahogens, 2006

Fluorescence microscopy of Vero cells infected with wild type L. monocytogenes (A) or L. monocytogenes DActA (B). Wild type L. monocytogenes induces the formation of actin comet tails that propulse the bacteria in the host cell cytoplasm (A) while L. monocytogenes DActA proliferates in micro-colonies that are unable to spread from the primary infected cell (B). DNA is labeled with DAPI (blue), bacterial cell wall is labeled with an anti-L. monocytogenes antibody (red) and actin is labeled with fluorescent phalloidin (green).