Images
Biologie cellulaire de l'infection par <i>Listeria monocytogenes</i>
Motilité intra-cellulaire
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Figure from Pizarro-Cerda et al. Cell Biology Handbook, 2006 Actin polymerisation and protrusion formation by Listeria. LoVo cells were infected with Listeria as described in the procedures. Both bacteria and the actin cytoskeleton were labelled for immuno-fluorescence analysis. (A) Labelling of the total population of bacteria associated to LoVo cells. (B) Actin labelling with the fungal drug phalloidin. While some bacteria are not associated with actin (small arrowheads), other bacteria are detected at early phases of actin polymerisation (small arrows). Bacteria at later stages of their intracellular cycle can be observed at the tip of actin comet tails (big arrows). Listeria that have reached the plasma membrane can form protrusions that extend beyond the infected cell (big arrowhead). (C) Phase image of LoVo cells and Listeria (bar: 5 µm). |