Images

Figure from Pizarro-Cerda et al. Cell Biology Handbook, 2006

Interaction of Listeria with its receptors E-cadherin and c-Met. LoVo cells were infected with Listeria monocytogenes as described in the procedures and its receptors E-cadherin and c-Met were immuno-labelled. (A) c-Met show a diffuse distribution in LoVo cells and is clearly absent at sites of adherens junctions formation, but its clearly recruited at sites of bacterial invasion. While some of the invading Listeria colocalise both with E-cadherin and c-Met, one bacterium is shown that only colocalises with c-Met and not with E-cadherin (arrowhead), suggesting that the interaction of Listeria with each one of their receptors can be uncoupled, as it is observed in cells lines such as Caco-2 (in which invasion is mainly mediated by the InlA/E-cadherin interaction) or Vero (in which invasion is driven exclusively by the InlB/c-Met interaction). (B) E-cadherin distribution at the plasma membrane of LoVo cells can be clearly seen at sites of adherens junctions formation as well as at sites of bacterial entry. (C) Phase image of LoVo cells and Listeria (bar: 5 µm). Opposing arrows: adherens junction. Big arrows: bacteria that recruit E-cadherin and c-Met at their site of entry. Arrowhead: bacterium that only recruits c-Met at its site of entry.