Images

Figure from Pizarro-Cerda J, Cossart P. J Pathol. 2006 Jan;208(2):215-23.

Figure 3. The InlB-invasion pathway. A. Schematic model of the molecules implicated in the InlB-invasion pathway. Membrane-bound InlB or released InlB interacts with its receptor, the hepatocyte growth factor receptor Met: this interaction leads to dimerization (not shown) and autophosphorylation of Met, which recruits the adaptor molecules Shc, Gab1 and Cbl to the bacterial entry site. These adaptors help to recruit phosphatidylinositol 3-kinase (PI3K) type I to the plasma membrane, inducing the formation of phosphatidylinositol 3,4,5-triphosphate and activation of the small GTP-binding protein Rac, both upstream of the cytoskeletal rearrangements required for bacterial entry. Cbl is also an ubiquitin ligase that induces the clathrin-dependent internalization of Met, favouring bacterial internalization. B. Immunofluorescence showing the recruitment of clathrin (red) at the bacterial entry site (intracellular bacteria green, extracellular bacteria blue, overlay of clathrin and intracellular bacteria, yellow). C. Three-dimensional reconstitution of the recruitment of clathrin by L. monocytogenes (colours similar to those in B).