Publications

2013

BAUMEL-ALTERZON S. WEBER C. GUILLEN N. ANKRI S. Identification of dihydropyrimidine dehydrogenase as a virulence factor essential for the survival of Entamoeba histolytica in glucose-poor environments. Cell MIcrobiol. 15:130-44. 

HON CC. WEBER C. SISMEIRO O. PROUX C. KOUTERO M. DELOGER M. DAS S. AGRAHARI M. DILLIES MA. JAGLA B. COPPEE JY. BHATTACHARYA A. GUILLEN N. Quantification of stochastic noise of splicing and polyadenylation in Entamoeba histolytica. Nucleic Acids Res. 41: 1936-52.

PERDOMO D. BARON B. ROJO-DOMINGUEZ A, RAYNAL B. ENGLAND P. GUILLEN N. The alpha-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells. 
Sci Rep. 3:1171.


2012


FAUST DM, GUILLEN N. Virulence and virulence factors in Entamoeba histolytica, the agent of human amoebiasis.
Microbes Infect. 14:1428-41.

GIRARD-MISGUICH F, DELGADO-ORTEGA M, BERTHON P, ROSSIGNOL C, LARCHER T, BRUEL T, GUIBON R, GUILLEN N, MEURENS F. Porcine colon explants in the study of innate immune response to Entamoeba histolytica
Vet Immunol Immunopathol. 145:611-7.

LOPEZ-ROSAS I, OROZCO E, MARCHAT LA, GARCIA-RIVERA G, GUILLEN N, WEBER C, CARRILLO-TAPIA E, HERNANDEZ DE LA CRUZ O, PEREZ-PLASENCIA C, LOPEZ-CAMARILLO C. mRNE decay proteins are targeted to poly(A)+ RNA and dsRNA-containing cytoplasmic foci that resemble P-bodies in Entamoeba histolytica. PLoS One, 7:e45966.

SANTI-ROCCA J, SMITH S, WEBER C, PINEDA E, HON CC, SAAVEDRA E, OLIVOS-GARCIA A, ROUSSEAU S, DILLIES MA, COPPEE JY, GUILLEN N. Endoplasmic reticulum stress-sensing mechanism is activated in Entamoeba histolytica upon treatment with nitric oxide. 
PLoS One, 7:e31777.

THIBEAUX R, DUFOUR A, ROUX P, BERNIER M, BAGLIN AC, FRILEUX P, OLIVO-MARIN J-C, GUILLEN N, LABRUYERE E. Newly visualized fibrillar collagen scaffolds dictate Entaboeba histolytica invasion route in the human colon. 
Cell Microbiol. 14:609-21.

URIBE R, ALMARAZ BARRERA MDE J, ROBLES-FLORES M. MENDOZA HERNANDEZ G, GONZALEZ-ROBLES A, HERNANDEZ-RIVAZ R, GUILLEN N, VARGAS M. A functional study of nucleocytoplasmic transport signals of the EhNCABP166 protein from Entamoeba histolytica. Parasitology, 139:1697-710.

 

2011

DUFOUR A, THIBEAUX R, LABRUYERE E, GUILLEN N, OLIVO J-C. 3-D active meshes: fast discrete deformable models for cell tracking in 3D Time-Lapse Microscopy. IEEE Trans Image Process.  20:1925-37.

FAUST D, MARQUAY MARKIEWICZ J, DANCKAERT A, SOUBIGOU G, GUILLEN N. Human liver sinuosidal endothelial cells respond to interaction with Entamoeba histolytica by changes in morphology, integrin signalling and cell death.
Cell Microbiol. 13:1091-106.

GIRARD-MISGUICH F, COGNIE J, DELGADO-ORTEGA M, BERTHON P, ROSSIGNOL C, LARCHER T, MELO S, BRUEL T, GUIBON R, CHEREL Y, SARRADIN P, SALMON H, GUILLEN N, MEURENS F. Towards the establishment of a porcine model to study human amebiasis. PLoS One. 6:e28795.

MARQUAY MARKIEWICZ J, SYAN S, HON CC, WEBER C, FAUST D, GUILLEN N. A proteomic and cellular analysis of uropods in the pathogen Entamoeba histolytica. PLoS Negl Trop Dis. 5:e1002.

TOVY A, HERTZ R, SIMAN-TOV R, SYAN S, FAUST D, GUILLEN N, ANKRI S. Glucose starvation boosts Entamoeba histolytica virulence. PLoS Negl Trop Dis. 5:e1247. 
 

 

2010

BRUEL T, GUIBON R, MELO S, GUILLEN N, SALMON H, GIRARD-MISGUICH F, MEURENS F.  Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica
Dev Comp Immunol. 34:562-71.

MAUGIS B, BRUGUES J, NASSOY P, GUILLEN N, SENS P, AMBLARD F. Dynamic instability of the intracellular pressure drives bleb-based motility. J Cell Sci. 15:3884-92.


2009

BANSAL D, AVE P, KERNEIS S, FRILEUX P, BOCHE O, BAGLIN AC, DUBOST G, LEGUERN AS, PREVOST M-C, BRACHA R, MIRELMAN D, GUILLEN N, LABRUYERE E.  An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis.
PLoS Negl Trop Dis. 3:e551.

LOPEZ-CAMARILLO C, LOPEZ-CASAMICHANA M, WEBER C, GUILLEN N, OROZCO E, MARCHAT LA. DNA repair mechanisms in eukaryotes: Special focus in Entamoeba histolytica and related protozoan parazites. Infect Genet Evol. 9:1051-6.

MEURENS F, GIRARD-MISGUICH F, MELO S. GRAVE A, SALMON H, GUILLEN N. Broad early immune response of porcine epithelial jejunal IPI-21 cells to Entamoeba histolytica. Mol Immunol. 46:927-36.


SANTI-ROCCA J, RIGOTHIER M-C, GUILLEN N.  Host-microbe interactions and defense mechanisms in the development of amoebic liver abscesses. Clin Microbiol Rev. 22:65-75.

SOLIS CF, SANTI-ROCCA J, PERDOMO D, WEBER C, GUILLEN N. Use of bacterially expressed dsRNA to downregulate Entamoeba histolytica gene expression. PLoS One, 4:e8424.

WEBER C, MARCHAT LA, GUILLEN N, LOPEZ-CAMARILLO C. Effects of DNA damage induced by UV irradiation on gene expression in the protozoan parasite Entamoeba histolytica. Mol Biochem Parasitol.164:165-9.


2008


BLAZQUEZ S, GUIGON G, WEBER C, SYAN S, SISMEIRO O, COPPEE JY, LABRUYERE E, GUILLEN N. Chemotaxis of Entamoeba histolytica towards the pro-inflammatory cytokine TNF is based on PI3K signalling, cytoskeleton reorganization and the Galactose/N-acetylgalactosamine lectin activity. Cell Microbiol. 10:1676-86.

JAIN R, SANTI-ROCCA J, PADHAN N, BHATTACHARYA S, GUILLEN N, BHATTACHARYA A. Calcium-binding protein 1 of Entamoeba histolytica transiently associates with phagocytic cups in a calcium-independent manner. Cell Microbiol. 10:1373-89.

GIRARD-MISGUICH F, SACHSE M, SANTI-ROCCA J, GUILLEN N. The endoplasmic reticulum chaperone calreticulin is recruited to the uropod during capping of surface receptors in Entamoeba histolytica. Mol Biochem Parasitol. 157:236-240.

MARCHAT LA, OROZCO E, GUILLEN N, WEBER C, LOPEZ-CAMARILLO C. Putative DEAD and DExH-box RNA helicases families in Entamoeba histolytica. Gene, 25:1-10.

SANTI-ROCCA J, WEBER C, GUIGON G, SISMEIRO O, COPPEE JY, GUILLEN N. The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis. Cell Microbiol. 10:202-17.

SOLIS CF, GUILLEN N. Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica.
Methods Mol Biol. 442:113-28.

WEBER C, BLAZQUEZ S, MARION S, AUSSEUR C, VATS D, KRZEMINSKI M, RIGOTHIER MC, MAROUN RC, BHATTACHARYA A, GUILLEN N. Bioinformatics and functional analysis of an Entamoeba histolytica mannosyltransferase necessary for parasite complement resistance and hepatical infection. PLoS Negl Trop Dis. 13:e165.

ZAMORANO, A, LOPEZ-CAMARILLO C, OROZCO E, WEBER C, GUILLEN N, MARCHAT LA. In silico analysis of EST and genomic sequences allowed the prediction of cis-regulatory elements for Entamoeba histolytica mRNA polyadenylation. 
Comput Biol Chem. 32:256-63.

ISAKOV E, SIMAN-TOV R, WEBER C, GUILLEN N, ANKRI S. Trichostatin A regulates peroxiredoxin expression and virulence of the parasite Entamoeba histolytica. Mol Biochem Parasitol. 158:82-94.

 

 

2007

 

RIVIERE C, MARION S, GUILLEN N, BACRI J-C, GAZEAU F, WILHELM C. Signaling through the phosphatidylinositol 3-kinase regulates mechanotaxis induced by local low magnetic forces in Entamoeba histolytica. J Biomech. 40:64-77.


BLAZQUEZ S, RIGOTHIER M-C, HUERRE M, GUILLEN N.  Initiation of inflammation and cell death during liver abscess formation by Entamoeba histolytica depends on activity of the galactose/N-acetyl-D-galactosamine lectin. Int J Parasitol. 37:425-33. 

SANTI-ROCCA J, WEBER C, GUIGON G, SISMEIRO O, COPPEE JY, GUILLEN N. 2007. The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis. Cell Microbiol. 10: 1373-89.

DIAZ-VALENCIA JD, ALMARAZ-BARRERA MD, JAY D, HERNANDEZ-CUEVAS NA, GARCIA E, GONZALEZ-DE LA ROSA CH, ARIAS-ROMERO LE, HERNANDEZ-RIVAS R, ROJO-DOMINGUEZ A, GUILLEN N, VARGAS M. Novel structural and functional findings of the ehFLN protein from Entamoeba histolytica. Cell Motil Cytoskeleton. 64:880-96.

CLARK CG, ALSMARK UC, TAZREITER M, SAITO-NAKANO Y, ALI V, MARION S, WEBER C, MUKHERJEE C, BRUCHHAUS I, TANNICH E, LEIPPE M, SICHERITZ-PONTEN T, FOSTER PG, SAMUELSON J, NOEL CJ, HIRT RP, EMBLEY TM, GILCHRIST CA, MANN BJ, SINGH U, ACKERS JP, BHATTACHARYA S, BHATTACHARYA A, LOHIA A, GUILLEN N, DUCHENE M, NOZAKI T, HALL N. Structure and content of the Entamoeba histolytica genome. Adv Parasitol. 65:51-190.

 

 

2006


MARION S, GUILLEN N. Genomic and proteomic approaches highlight phagocytosis of living and apoptotic human cells by the parasite Entamoeba histolytica. Int J Parasitol. 36 : 131–139. 

BLAZQUEZ S, ZIMMER C, GUIGON G, OLIVO-MARIN JC, GUILLEN N, LABRUYERE E. Human tumor necrosis factor is a chemoattractant for the parasite Entamoeba histolytica. Infect Immun. 74:1407-11. 

LABRUYERE E, GUILLEN N.  Host Tissue Invasion by Entamoeba histolytica Is Powered by Motility and Phagocytosis. Arch Med Res. 37:252-7. 

WEBER C, GUIGON G, BOUCHIER C, FRANGEUL L, MOREIRA S, SISMEIRO O, GOUYETTE C, MIRELMAN D, COPPEE JY and GUILLEN N. Stress by heat shock induces massive down regulation of genes and allows differential allelic expression of the Gal/GalNAc lectin in Entamoeba histolytica Euk. Cell, 5: 871–875.

 

 

 2005


TAVARES, P., RIGOTHIER, M-C., KHUN, H., ROUX, P., HUERRE, M. GUILLEN N. The role of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance. Infect Immun. 73:1771-1778. 

AGUILAR-ROJAS A. ALMARAZ-BARRERA M-J. ROBLES-FLORES M. HERNANDEZ-RIVAS R. GUILLEN N. MAROUN R. VARGAS M.  Inhibition of phatogenic mechanisms of Entamoeba histolytica by overexpression of EhGEF1: a novel Rho/Rac guanine nucleotide exchange factor. Exp. Parsitol. 109:150-62. 

SEIGNEUR M. MOUNIER J. PREVOST M-C. GUILLEN N.  A lysine- and glutamic acid-rich protein, KERP1, from Entamoeba histolytica binds to human enterocytes. Cell Microb 7 : 569–579 

LOFTUS B, ANDERSON I, DAVIES R, ALSMARK UC, SAMUELSON J, AMEDEO P, RONCAGLIA P, BERRIMAN M, HIRT RP, MANN BJ, NOZAKI T, SUH B, POP M, DUCHENE M, ACKERS J, TANNICH E, LEIPPE M, HOFER M, BRUCHHAUS I, WILLHOEFT U, BHATTACHARYA A, CHILLINGWORTH T, CHURCHER C, HANCE Z, HARRIS B, HARRIS D, JAGELS K, MOULE S, MUNGALL K, ORMOND D, SQUARES R, WHITEHEAD S, QUAIL MA, RABBINOWITSCH E, NORBERTCZAK H, PRICE C, WANG Z, GUILLEN N, GILCHRIST C, STROUP SE, BHATTACHARYA S, LOHIA A, FOSTER PG, SICHERITZ-PONTEN T, WEBER C, SINGH U, MUKHERJEE C, EL-SAYED NM, PETRI WA JR, CLARK CG, EMBLEY TM, BARRELL B, FRASER CM, HALL N. The genome of the protist parasite Entamoeba histolytica. Nature. 433:865-8. 

MARION S, GUILLEN N, BACRI J-C, WILHELM C. Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm. Eur Biophys J. 34:262-72 

MARION S.  LAURENT C. GUILLEN N.  Signalization and cytoskeleton Activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach. Cell Microbiol. 7:1504-18. 

DUFOUR A. SHIHIN,V. TAJBAKHSH, S. GUILLE, N.,OLIVO-MARIN J-C. ZIMMER C. Segmenting and tracking fluorescent cells in dynamic 3-D microscopy with coupled active surfaces. IEEE Trans. Image Process. 14:1396-1410. 

DIAZ-VALENCIA JD. ALMARAZ-BARRERA MDE J. ARIAS-ROMERO LE. HERNANDEZ-RIVAS R. ROJO-DOMINGUEZ A. GUILLEN N. VARGAS M. The ABP-120 C-end region from Entamoeba histolytica interacts with sulfatide, a new lipid target.
Biochem Biophys Res Commun. 33 :1527-36.

 

  

2004


MARION S, TAVARES P, ARHETS P, GUILLEN N. Signal transduction through the Gal-GalNAc lectin of Entamoeba histolytica involves a spectrin- like protein. Mol. Biochem Parasitol. 135:31-38 

MARION S, WILHEM C, BACRI J-C, GUILLEN N. Overexpression of myosin IB in living Entamoeba histolytica enhances cytoplasm viscosity and reduces phagocytosis. J. Cell Sci. 117:3271-79 

SAHOO N, LABRUYERE E, BHATTACHARYA S, SEN P, GUILLEN N, BHATTACHARYA A.  Calcium binding protein 1 of the protozoan parasite Entamoeba histolytica interacts with actin and is involved in dynamics of cytoskeleton.
J. Cell Sci. 117:3625-34 

COUDRIER E, AMBLARD F, ZIMMER C, ROUX P, OLIVO-MARIN J-C, RIGOTHIER M-C, GUILLEN N.  Myosin II and the Gal-GalNAc lectin play a crucial role in tissue invasion by Entamoeba histolytica. Cell Microbiol. 7:19-27.


VAYSSIE L,VARGAS M, WEBER C, GUILLEN, N.  Double stranded RNA mediate homology dependant gene silencing of g-tubulin in the human parasite Entamoeba histolytica. Mol. Biochem. Parasitol. 138: 21-2860

 

 

2003


LABRUYÈRE E, ZIMMER C, GALY V, OLIVO-MARIN J-C, GUILLEN N. EhPAK, a member of the p21-activated kinase family is involved in the control of Entamoeba histolytica migration and phagocytosis. J. Cell Sci. 116/61-71 

CHAVEZ-RIOS R, ARIAS-ROMERO LE, DE JESUS ALMARAZ-BARRERA M, HERNANDEZ-RIVAS R, GUILLEN N, VARGAS M.  L10 Ribosomal Protein from Entamoeba histolytica Share Structural and Functional Homologies with QM/Jif-1 Transcription Factor. Mol. Biochem. Parasitol. 127/151-60.

 

2002


RIGOTHIER M-C, KHUN H, TAVARES P, CARDONA A, HUERRE M, GUILLEN N. The fate of Entamoeba histolytica during establishment of amoebic liver abscess analyzed by quantitative radio-imaging and histology. Infect. Immun. 70, 3208-3215. 

ZIMMER C, LABRUYÈRE E, MEAS-YEDID V, GLORY E, GUILLEN N, OLIVO- MARIN J-C. Segmentation and Tracking of Migrating Cells in Videomicroscopy With Parametric Active Contours: A Tool for Cell-Based Drug Testing. IEEE Transactions on Medical Imaging, 21/1212-1221.