Offres de stages postdoctoraux à l'Institut Pasteur

22/01/2013 - A funded postdoctoral position is available immediately in the Molecular Mycology unit directed by Francoise Dromer at the Institut Pasteur in Paris.

 16/01/2013 - Identification of novel regulators of senescence

 

Institut Pasteur Unit Oncogenesis and Nuclear Organization, INSERM U993

(Director : Anne DEJEAN ; Principal Investigator : Oliver BISCHOF)

Institut Pasteur, Paris.

Applications are invited to apply for a postdoctoral research position within the Oncogenesis and Nuclear Organization Unit at the Pasteur Institut in Paris. We are looking for a talented and motivated post-doctoral student interested in joining our team. The successful candidate will have the opportunity to work on one of several open projects involving the identification and characterization of the senescence phenotype with an emphasis on chromatin structurefunction and non-coding RNAs.

Candidates should be highly motivated, committed and have clear career goals.

Experience in senescence and cancer research would be an asset but are not crucial. A strong background in molecular and cellular biology techniques with a special emphasis on chromatin structure-function analysis and noncoding RNAs are essential. Moreoever, the candidate should have had significant exposure to the bioinformatic analysis of NGS data and should be familiar with culturing of primary cells, retroviral and lentiviral infections and sh/siRNA-mediated silencing. All applicants should have a Ph.D. degree in life sciences or equivalent experience and minimum one first-author publication published or in press in peerreviewed international journals. Institut Pasteur provides an excellent research infrastructure including many core facilities and support structures. For informal enquiries regarding the posititon, please, contact Dr. Oliver BISCHOF at obischof@pasteur.fr or +33140613307.

Please, send CV, letter of motivation and name/address of 3 referees to Dr. Oliver BISCHOF (obischof@pasteur.fr). or by regular mail to D. Oliver BISCHOF Institut Pasteur, Unité d'Organisation Nucleaire et Oncogenese (ONO), INSERM U993, Bat. Andre Lwoff, 28, rue du Dr Roux, 75724 Paris cedex 15, France.

Closing date : March 2013

Interviews: April 2013.

Recent Publications :

1. A New Player in PML-mediated Cellular Senescence: TBX2 Gets into the Loop (2012). Martin N, Dejean A, Bischof O. Med Sci (Paris). 2012 Mar;28(3):248-50.

2. Benhamed, M., Herbig, U. Dejean, A. and Bischof, O. (2012). Senescence is an Endogenous Trigger for MicroRNA-Mediated Transcriptional Gene Silencing in Human Cells. Nat. Cell Biol. 26;14(3):266-75. doi: 10.1038/ncb2443.

3. Nadine Martin, Moussa Benhamed, Karim Nacerddine, Maud Demarque, Maarten van Lohuizen, Anne Dejean and Oliver Bischof. (2011). Physical and Functional Interaction between PML and TBX2 in Cellular Senescence EMBO J. 14;31(1):95-109.

4. Martin, N., Schwamborn, K., Schreiber, V., Werner, A., Guillier, C., Zhang, X.D., Bischof, O., Seeler, J.S., Dejean, A. (2009). PARP-1 transcriptional activity is regulated by sumoylation upon heat shock. EMBO J. 22:3534-48.

5. Bischof, O., Pineau, P., and Dejean A. (2009). A Re-View of Cellular Senescence : Friend or Foe in Tumor Suppression. Médicine et Sciences, 25 :153-60.

6. Carter, S., Bischof, O., Dejean, A. and Vousden, K. (2007). C-terminal modifications regulate MDM2 dissociation and nuclear export of p53. Nat. Cell Biol., 9: 428-435.

7. Bischof, O. and Dejean, A. (2007). SUMO is Growing Senescent. Cell Cycle 6, 677-681.

8. Kumar, P.*, Bischof, O.*, Purbey, P.K., Notani, D., Urlaub, H., Dejean, A. and Galande, S. (2007).
Functional Interaction between PML and SATB1 Regulates Chromatin Loop Architecture and Transcription of the MHC class I Locus. (* Co-first authors). Nat. Cell Biol., 9:45-56.

9. Seeler J.S., Bischof O., Nacerddine K. and Dejean A. (2007). SUMO, the three Rs and Cancer.Acute Promyelocytic Leukemia: Mechanisms and Targets. Curr. Top. Microbiol. Immunol., 313:49-71.

10. Bischof, O., Schwamborn, K., Martin, N., Werner, A., Sustmann, C., Grosschedl, R. and Dejean, A. (2006). The E3 SUMO Ligase PIASy is a Novel Regulator of Cellular Senescence and Apoptosis. Mol Cell 22, 783-794.

18/12/2012 - 2-years post-doctoral position in Molecular and Cellular Virology

Description

A 2-years post-doctoral position is available at Institut Pasteur, in the group « Arboviral virulence factors and toxins », which is part of the Structural Virology unit. The position is financed in the context of a collaborative program between Institut Pasteur and Institut Mérieux.

The aim of the project is to further define the contribution of the dengue virus (DENV) nonstructural protein NS1 to viral pathogenesis. NS1 is a multifunctional protein that localizes to replication factories inside infected cells and is secreted in the extracellular fluid as a soluble hexameric particle (NS1^hex). We recently reported that secreted NS1^hex is an atypical high density lipoprotein that could possibly interfere with the vascular system during the course of infection (Gutsche et al., PNAS 2011). We also found that DENV NS1^hex associates to host proteins during its circulation in the extracellular fluid. We propose to further characterize the nature of these complexes by different approaches (in vitro reconstitution, immunoassays, mass spectrometry, electron microscopy). The role of these complexes will be studied in relevant target cells such as hepatocytes and their prognostic value will be assessed in human patients, in collaboration with the Institut Pasteur International Network.

Qualification

The applicant should have a PhD with at least 3 years of post-doctoral experience in the field of virology/infectious diseases, and a strong background in biochemistry, molecular and cellular biology. A specific expertise on membrane proteins would be particularly appreciated. Good written and oral communication skills in english are required. The position is expected to start February 1st, 2013 at the latest.

Salary will depend on experience and past achievements.

Application

Applications should include a CV, a summary of previous research activities (2-3 pages) and 2 references (just include name and contact information), to be addressed by e-mail before January 10th, 2013 to

Marie Flamand (marie.flamand@pasteur.fr)

14/12/2012 - CRBIP (Biological Resources Center of Institut Pasteur) is looking for a project manager .

CRBIP is in charge of the valorisation of biological resources of the Institut Pasteur which are available in the collections. CRBIP is involved in national and international projects in order to facilitate access to biological resources of high quality and help in the creation of BRC networks. CRBIP is a member of the team management for these different projects.

Activities :

-     operational management of national and European projects 
-     business plan creation and follow-up of WP regarding MIRRI project and different CRBIP’s projects 
-     participation to national and international coordination
-     writing scientific reports of projects
-     follow-up of financial reports of projects
-     communications (newspapers relations and public relations) bounded to projects : news, information, proposal on communication possibilities
-     participation to national and European meetings 

Skills :

-          Very good knowledge in English (read, written, spoken)
-          Fair knowledge of pack office
-          Experience in project management
-          Good level in writing processes and scientific information
-          Team working
-          Diplomatic behaviour and listening ability
-          Initiatives capacity, reactivity and  adaptability are demanded

Job descriptions :

 -     Post doc
-      Full time
-      Managed by CRBIP’s head office.
-      5 years experience in the same position
-      Position opened in January 2013 for 2 years.
 

03/12/2012 - Postdoc position on transcriptomics of mosquito-virus interactions at Institut Pasteur, Paris

The goal of the project is to use massively parallel transcriptome sequencing (RNA-seq) to identify and characterize genes involved in the vector competence of field-derived Aedes aegypti mosquitoes for low-passage dengue virus isolates. It will consist of identification of candidates genes by genome-wide transcriptomic analysis followed by functional validation using gene-silencing experiments in vivo.

The project relies on a tight collaboration between the research groups of Dr. Louis Lambrechts (Insects & Infectious Diseases) and Dr. Carla Saleh (Viruses & RNA interference). The successful candidate will interact with both teams and be in charge of (1) statistical analysis of read counts data; (2) quantitative RT-PCR validation of gene expression data; (3) vector competence and gene silencing assays in vivo. The position requires an independent, highly motivated, creative, and scientifically curious individual. We are looking for a team player with excellent communication skills and an international outlook.

Applicants must have demonstrated expertise in the handling and statistical analysis of genome-wide transcriptomic data, and a strong background in molecular biology. Additional experience in virology and/or entomology is desirable but not essential. Particularly encouraged are applicants with prior experience in Illumina RNA-seq. Net salary will be ~24k€/year including health benefits. Candidates must hold a PhD in a relevant scientific field. Knowledge of the French language is not required. There is no age or nationality restriction.

Formal applications should include: a cover letter (in English) indicating research interests, your CV including a summary of previous research, and two letters of reference. Applications and informal enquiries must be submitted by email to:louis.lambrechts@pasteur.fr and carla.saleh@pasteur.fr

Information on the current activities of the labs can be found at:

http://www.salehlab.eu/ and http://louis.lambrechts.free.fr/

15/10/2012 - Microbes and Host Barriers Group, Institut Pasteur, Inserm, Paris, France

A funded postdoctoral position is available in the "Microbes and Host Barriers group" directed by Marc Lecuit at the Institut Pasteur in Paris.

The project focuses on Chikungunya virus (CHIKV), a recently re-emerged arbovirus transmitted to human by mosquito bite that belongs to the Alphavirus genus. It was responsible in 2006 for a massive outbreak, first in India and the islands of the Indian Ocean including the island of La Réunion, causing more than one million of cases of CHIKV infection. CHIKV is now regarded as the arbovirus most likely to spread globally, given the wide distribution of its mosquito vector. CHIKV induces a relatively mild disease in human, characterized by fever, arthralgia, myalgia and rash. Chronic and relapsing arthralgia is observed in a significant proportion of patients. Cases of severe CHIKV infection with central nervous system involvement have also been described, particularly in neonates born to viremic mothers. Neither vaccine nor therapy against CHIKV is commercially available.

We first developed an animal model to study the pathophysiology of infection, and its cell and tissue tropisms, and the resulting host responses. We now pursue our studies on CHIKV by focusing on the cell biology of this poorly studied Alphavirus.

The objective of the project is to identify the cellular genes involved in CHIKV replication. Genome-wide loss-of-function screens using small interfering RNA (siRNA) libraries and automation technologies have been conducted to discover host factors participating in the lifecycle of several viruses. We have followed a similar functional genome-wide RNAi screen approach to identify the cellular genes required for CHIKV entry and replication as well as those involved in the inhibition or the promotion of viral replication in human cells, in collaboration with Thomas Meyer (Max Planck Institute, Berlin, Germany). The project will consist in investigating in detail the role of genes identified by the whole genome screen. Once their role confirmed by independent experiments with individual siRNAs, their contribution to the infection process will be deciphered: their role in CHIKV infection will be analyzed by biochemical and molecular approaches to reveal their molecular mechanisms of actions. Interactions between cellular and viral gene products implicated in CHIKV replication will be characterized and analyzed using high-resolution imaging. The impact of the identified host gene products on CHIKV infection will be assessed in vivo in mouse models.

This study of the cell biology of this recently re-emerged virus will not only contribute to a better understanding of the basic biology of this virus, but will also lead to the identification of novel targets for the development of new antiviral drugs.

Candidates profile:

PhD, virology, cell biology.
Skills in molecular biology and biochemistry. Experience in in vitro culture and microscopy wouldbe appreciated.
Applicants should ideally possess a strong background in molecular biology, biochemistry and virology, be highly motivated and show an ability to work within a team. Our group enjoys numerous collaborations with researchers on campus and beyond. The Institut Pasteur campus hosts expert teams in cell biology, virology, molecular biology and microbial pathogenesis, as well as cutting-edge technical platforms, all of which will be essential for the success of the project.

Please send your application letter and a copy of your CV to marc.lecuit@pasteur.fr or therese.couderc@pasteur.fr

26/09/2012 - Membrane Traffic and Cell Division Laboratory

Institut PASTEUR, Paris, FRANCE

A funded postdoctoral position is currently available in the "Membrane Traffic and Cell Division laboratory" of Dr. Echard at the Institut Pasteur in Paris. This position has no nationality restriction and the working language is English.

Our research focuses on the role of membrane trafficking in lipid and cytoskeleton remodeling at each step of animal cell division, under normal and pathological conditions.  We are particularly interested in cytokinesis, the final step of cell division leading to the physical separation of the daughter cells. Using cells, high-content RNAi-based screens, state-of-the-art live-cell imaging and advanced microscopy techniques, we are exploring the novel and promising interfac between cytokinesis and membrane traffic at the cell and tissue level. Our recent work revealed unexpected connections between the human Lowe syndrome, endocytosis, cytoskeleton and cell division.

We are seeking a highly motivated candidate for joining our dynamic and international team with:
- a PhD and 0-3 years of relevant postdoctoral experience
- a strong track-record of publications in peer-reviewed journals
- validated experience in cell biology, RNAi-based screens, mouse models, lipid biology and/or cell imaging.

Please send your application as a single PDF document containing a cover letter, a concise summary of previous research, a Curriculum Vitae, a publication list and contact details for at least 2 references to arnaud.echard@pasteur.fr

For further information, please contact arnaud.echard@pasteur.fr and visit our website: http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-departments/cell-biology-andinfection/

Selected Recent Publications:

An ARF6/Rab35 GTPase Cascade for Endocytic Recycling and Successful Cytokinesis.
Chesneau L, Dambournet D, Machicoane M, Kouranti I, Fukuda M, Goud B, Echard A.
Current Biology: 22, 147-53 (2012)

Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis.
Dambournet D., Machicoane M., Chesneau L., Rocancourt M., Formstecher E., Salomon R., Goud B. and Echard A.
Nature Cell Biology: 13, 981-8 (2011)

Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex.
Miserey-Lenkei S, Chalancon G, Bardin S, Formstecher E, Goud B, Echard A.
Nature Cell Biology: 12, 645-54 (2010)

11/09/2012 Postdoctoral position in Virology Pasteur Institute

Description:
A 48 month postdoctoral position is available starting December 2012 to join a newly established research program within the Department of Virology of the Pasteur Institute, Paris.

Project outline:
Very early upon virus infection of mammalian cells, many anti-viral genes, collectively called as "viral stress-inducible genes' are activated directly by pathogen-associated molecular patterns (PAMPs) without the need of Interferon (IFN) signaling. The project aims at understanding the molecular mechanisms and the dynamics of this IFN-independent pathway in the context of infection by medically relevant viruses. A better understanding of this arm of the innate immune response should shed some light on the control of virus replication by the host cell, the cell tropism of the virus and the development of disease.

Key words:
dynamics of virus-host interaction, innate immunity, viral stress-inducible genes.

Candidate requirements:
Candidates must hold a PhD degree in Biological Sciences since less than 2 years. They must have experience in the domain of mammalian virology, innate immunity and cell biology. Experience in live cell microscopy will be advantageous. Candidates should have strong communication and organization skills and willing to working independently. French language is not required.

To apply:
Applicants should send a cover letter and a C.V (including a list of publications as well as names and contact information for up to 3 academic references)to Nolwenn Jouvenet (nolwennjouvenet@gmail.com).

• Description

• Qualification

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09/02/2012 - Postdoctoral position in the Laboratory of Trypanosoma Infectious
ProcessesKey words

Two postdoctoral positions are available in the Innate Immunity Unit (http://www.pasteur.fr/recherche/UPDATE) in the Immunology Department at the Institut Pasteur starting January 2012 utilizing 'humanized' mice harboring human lympho-hematopoietic systems for projets relating to preclinical studies of human immunology. HIS mouse models provide an opportunity to decipher the molecular mechanisms controlling human lymphocyte development and function. Progress in this area has been rapid (Manz and Di Santo, Nat Immunol 2009) and research and clinical applications for HIS mice are attracting industrial partners at an unprecendented rate. Three-year positions are funded through the ANR (program RPIB, project 'Im_HIS').

The Innate Immunity Unit has a long-standing interest in the development of xenograft mouse models for the analysis of immune responses to pathogens and cancer. Thanks to a previous award from the Bill and Melinda Gates Foundation (Grand Challenges in Global Health), a series of innovative immunodeficient recipient strain for HIS mouse generation were developed (Huntington et al, JEM 2009; Huntington et al PNAS 2011; Legrand et al PNAS 2011). The successful applicant will use these new HIS models in order to model and study the pathogenesis of human infectious diseases, using state-of-art instruments and in vivo approaches available in the lab and across the institute.

QUALIFICATIONS: Interested candidates must be completing or should have obtained a Ph.D. or M.D. degree with background in Immunology, Molecular Biology, Cell Biology, Physiology or a related discipline. The applicant should have record of delivering high-quality research using immunology, molecular biology, and/or animal models. Experience in transcriptional analysis, multi-parameter flow cytometry and/or mouse models are highly desirable.

SALARY/BENEFITS: These positions are fully supported by the ANR and successful candidates will be offered competitive stipend/salary and benefits commensurate with experience and accomplishments.

TO APPLY: E-mail your CV and at least 3 references to James Di Santo (james.di-santo@pasteur.fr) by November 15, 2011.

Prof. Simon Wain-Hobson

Molecular Retrovirology Unit

Institut Pasteur, Paris

A 3 year post-doc doctoral position is available. The project is financed in the context of a Medicen-funded project TelVac and involves two companies, and three basic research labs. The post-doctoral work will be carried out in a research lab at Institut Pasteur.

Hypermutation follows from extensive deamination of cytidine residues in DNA and was initially thought to be confined to viral genomes (Vartanian et al. Science 320, 230 (2008); Vartanian et al. PLoS Pathogens 6, e1000928 (2010)). More recently we have shown that mitochondrial can be dited by several APOBEC3 enzymes, and more importantly, nuclear DNA can be hypermutated (Suspène et al. 108, 4858 (2011)). And of course, somatic mutation brings us to cancer. This ties in well with the large number of GC->AT mutations that characterise cancer genomes. This is now the major research interest of the lab.

The project seeks to define the APOBEC3 transcriptome as well as those of related genes and interactors in lung cancer and peritumoral tissue. Considerable tissue culture work on the promoter and interactors will be performed to consolidate and explore the in vivo findings. A good knowledge of molecular and cellular biology is useful.

As part of a research program that involves several laboratories of the Ile-de-France Region, a post-doctoral position is available for a two-year period in the research group of Dr. Nicolas Escriou (Viral Genomics and Vaccination Unit, Institut Pasteur, Paris, France). The program aims at studying the mechanisms of adaptation of pandemic A/H1N1v (2009) influenza virus to humans. Notably, the program will explore the contribution of innate immunity responses and identify viral determinants involved in this process.

The post-doctoral fellow will be responsible for the development and implementation of the guinea pig animal model, and will contribute to the development of the viral reverse genetics system. He/She will perform transcriptome analyses in infected cells and animals. This program involves experiments in BSL2 and BSL3+ research facilities.

The research interests of the Viral Genomics and Vaccination unit, headed by Dr Frédéric Tangy at the Institut Pasteur in Paris, are focused on the study of functional virus-host interactions, virulence determinants and their role in pathogenesis, as well as the development of innovative therapeutic and prophylactic strategies based on measles virus and targeting notably AIDS and respiratory viruses. The unit is part of CNRS URA 3015.

Dans le cadre d’un Programme collaboratif financé par la région Ile de France réunissant plusieurs équipes franciliennes, un poste de chercheur post-doctoral (géré par le CNRS) est ouvert pour 24 mois dans l’équipe du Dr. Nicolas Escriou (Unité de Génomique Virale et Vaccination, Institut Pasteur, Paris, France). Le programme consiste en l’étude de mécanismes d’adaptation du virus grippal pandémique A/H1N1v (2009) à l’homme, et notamment en l’évaluation du rôle de la réponse immunitaire innée et l’identification des déterminants viraux associés.

Le chercheur post-doctoral aura la responsabilité de la mise au point et de la mise en oeuvre du modèle animal cobaye nécessaire au programme et il contribuera au développement des systèmes de génétique inverse virale ainsi qu’à la réalisation des analyses transcriptomiques chez l’animal et dans la cellule infectée. Ce programme implique la mise en oeuvre d’un ensemble de technologies variées en milieu confiné de niveaux 2 et 3+.

Les activités de l'unité de Génomique Virale et Vaccination, dirigée par le Dr Frédéric Tangy à l’Institut Pasteur à Paris, sont centrées sur l’étude des interactions fonctionnelles virus / hôte, l'analyse des déterminants de virulence et des processus pathologiques associés, ainsi que le développement de nouvelles stratégies thérapeutiques et prophylactiques reposant sur le vecteur rougeole, notamment pour le virus du SIDA et les virus respiratoires. L’unité fait également partie de l’URA 3015 du CNRS.

A two year position funded by the French National Research Agency (ANR) is available in the Unit Cytokines & Inflammation at Institut Pasteur Paris, starting september 2011. The project is to study the role of intracellular TLR9 trafficking and signaling in an inflammation model. Studies will focus on the analysis of the inflammatory responses in TLR9, Asparagine endopeptidase (AEP) and Cathepsin L (CatL)deficient mice in the course of infection by Leishmania major. The role of these proteases in endosomal TLR stimulation has been previously shown. Their role in the trafficking of TLR and their relevance for the onset of innate and adaptive immune responses in L. major infection will be now investigated.

TLR9-deficient mice (TLR9^-/-) are more susceptible to infection with L. major than wild type (WT) C57BL/6 mice. TLR9^-/-mice resolve their lesions and control parasites growth with a much lower efficiency than WT. Preliminary data suggest that, in vitro, DCs lacking AEP activity infected by L. major are unable to respond, due to the impaired processing of TLR9. TLR9 processing is impaired in macrophages isolated from Catl deficient mice but not in macrophages lacking AEP. We will compare the susceptibility of AEP^-/-, TLR9-/-and CatL^-/-mice following inoculation by L. major promastigotes.

In order to establish the impact of TLR9 signaling in DCs and macrophages, we will investigate the development of cutaneous lesion and parasites burden after infection, until the healing of the lesions. We will analyse the recruitment of DCs, macrophages, neutrophils and T lymphocytes as well as proinflammatory cytokines and chemokines expression at the site of infection and in the draining lymph nodes. The orientation of the T cell response by measuring the expression of IFN-γ and IL-4 in T lymphocytes during the course of infection in TLR9, AEP, and CatL deficient mice will be also compared. Purified vertebrate DNA is unable to stimulate TLR9 but it has already been shown that the DNA from some protozoan parasites activate macrophages. Purified L. major DNA also induces the activation of DCs but the mechanism of such activation remains unsolved. Our plans are to determine the origin of the specific TLR9 dependent activation caused by L. major DNA in DCs and in macrophages.

This project is part of an ANR grant investigated by B. Manoury (Necker hospital U1013, coordinator of the project) N. Doyen and L. Touqui, (from the Department of Infection and Epidemiology of Insitut Pasteur, unit Cytokines & Inflammation and unit Innate host defense and Inflammation U 874), respectively.

This project will give important new insights in the field of leishmania research. Annual incidence of cutaneous leishmaniasis is estimated at 1-1.5 million cases worldwide. Overall prevalence of the disease is 12 million people and the population at risk is 350 million people.

The Institut Pasteur, located in the heart of Paris, provides an international and dynamic research environment with state-of-the art imaging and animal colonies facilities.

The applicant should have obtained his/her PhD less than 2 years ago.
The candidate should have strong experience in cell biology, molecular biology , cellular imaging and conceptual
training in immunology and parasitology. The working language is English; strong written and oral
communication skills are required.
 

Funding

CNRS postdoc salary scale (2 years) .

Contact

Please send full CV including research interests and 2 or 3 references with contact information to:
Dr Noëlle DOYEN
Group E3 In Unit of Cytokines and Inflammation
28 rue du Docteur Roux
75724 PARIS Cedex 15
http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-departments/infection-and-epidemiology/units-and-groups/cytokines-e-inflammation/index
email: noelle.doyen@pasteur.fr

Description

A postdoctoral position is available in the Innate Immunity Unit  in the Immunology Department at the Institut Pasteur starting January 2011 to study the molecular mechanisms involved in development and function of innate lymphoid cells (ILCs). ILCs are an emerging family of hematopoietic effector cells that play important roles in immunity and tissue remodeling during fetal and adult life (Spits and Di Santo, Nature Immunology 2010, in press). This three-year position is funded through the ANR (program Blanc, project ’Gut_ILC’).
The Innate Immunity Unit is interested in understanding the molecular events that generate functionally diverse ILC subsets and the roles for these innate effectors in tissue homeostasis, and immune responses against infectious pathogens and cancer. The laboratory has a long-standing interest in natural killer (NK) cell development and function (Di Santo, JP. Annu Rev Immunol 2006) and more recently has discovered novel populations of innate IL-22-producing cells that intervene at mucosal surfaces (Satoh-Takayama et al, Immunity 2008; Satoh-Takayama et al, JEM 2010). The successful applicant is expected to study the molecular mechanisms of ILC development and the roles for ILC subsets in animal models of diseases, using state-of-art instruments and in vivo approaches available in the lab and across the institute. 

QUALIFICATIONS

Interested candidates must be completing or should have obtained a Ph.D. or M.D. degree with background in Immunology, Molecular Biology, Cell Biology, Physiology or a related discipline. The applicant should have record of delivering high-quality research using immunology, molecular biology, and/or animal models. Experience in transcriptional analysis, multi-parameter flow cytometry and/or mouse models are highly desirable but not required. 

SALARY/BENEFITS

These positions are fully supported by the ANR and successful candidates will be offered competitive stipend/salary and benefits commensurate with experience and accomplishments. 

TO APPLY 

 E-mail your CV and at least 3 references to James Di Santo (disanto@pasteur.fr) by December 15, 2010.

Description

The project will investigate the role in vivo of chemokine immobilisation on proteoglycans, using the essential chemokine CXCL12 as the paradigm of haptotactic proteins. In particular, the project will explore the role of chemokine/proteoglycan interactions in homeostasis of bone-marrow homing of haematopoietic cells, lymphocyte trafficking and pathological situations associated with tissue regeneration, where CXCL12 plays a hierarchic role of paramount importance. To this purpose an animal model where the chemokine CXCL12 has been selectively disabled to bind on glycosaminoglycans while maintaining intact the agonist capacity, has been developed. The animal model is already available and relies in a number of preliminary results probing the existence of an original phenotype. Furthermore, the project will assess the role played by CXCL12/HS interactions in the physiopathology of tissue repair, and analyze the therapeutic potential of CXCL12 isoforms differing by their capacity to interact with heparan-sulphate proteoglycans.

The project will be conducted under the supervision of Fernando Arenzana-Seisdedos, head of the INSERM U819 unit and Viral Pathogenesis laboratory at the Institut Pasteur in Paris. The project is supported by a 2010-grant from the Agence National pour la Recherche 2010 (ANR-BLANC/Chemrepair).

Experience and skills of the candidate.

The applicant is expected to be highly motivated and enthusiastic and to work independently. The candidate will be in charge, as a priority, of the immunological aspects of the program and subsequently, of the exploration of the regenerative capacities of the muscle in the mutant animals. A strong background in immunology and confirmed expertise animal models are requested. Experience in cellular biology and microscopy imaging techniques will be appreciated and positively evaluated. Gross Salary: 2300 euros/month. Starting from October 2010.

Contact

Applications should be addressed to :
Dr. Fernando Arenzana-Seisdedos
INSERM U818, Institut Pasteur
28, rue Dr. Roux,
Paris 75724 cedex 15.

e-mail address: farenzan@pasteur.fr
telephone: 33 145688263
fax: 33 145688941

Description

 L’étude des mécanismes qui régissent l’entrée du Virus de l’Immunodéficience Humaine de type 1 (VIH-1) dans les cellules cibles est une activité récurrente de notre laboratoire. Lors de cette étape, la sous-unité de surface de la glycoprotéine d’enveloppe du VIH-1, ou gp120, interagit avec le récepteur CD4 puis selon le tropisme viral, avec le corécepteur CCR5 (virus R5) ou CXCR4 (virus X4 ou R5/X4). Les virus R5 sont préférentiellement transmis et prévalent pendant la phase chronique, asymptomatique de l’infection, alors que ceux qui utilisent CXCR4 émergent après quelques années chez la moitié des individus infectés, ce qui est concomitant à l’apparition des signes cliniques du SIDA. Les corécepteurs appartiennent à la famille des récepteurs couplés aux protéines G et sont les récepteurs de chimiokines capables d’activer les protéines G et de mobiliser diverses signalisations intracellulaires. La liaison des chimiokines sur les corécepteurs inhibe aussi l’entrée virale parce qu’elle occlue stériquement celle de la gp120 et parce qu’elle conduit à l’endocytose du corécepteur. Des données de notre laboratoire et de la littérature suggèrent cependant que les virus R5 échappent à l’inhibition par les chimiokines de CCR5 (R5-CCKs) au cours de l’infection. L’objectif principal de ce projet sera d’identifier les mécanismes moléculaires qui sous-tendent le développement de la résistance des virus R5 à l’action des chimiokines au cours de l’infection.
A cette fin, les gp120 d’isolats primaires issus d’un suivi longitudinal sur plusieurs années seront clonées et séquencées. Nous évaluerons l’hypothèse que des variations de la composition nucléotidique du gène env des virus R5 au cours de l’infection VIH sont associées à des modifications de l’efficacité de certaines étapes de l’entrée virale qui pourraient rendre compte de la résistance de ces virus aux R5-CCKs. Pour cela, nous mettrons notamment en œuvre des expériences de pharmacologie et de virologie moléculaires pour la caractérisation des propriétés de liaison aux récepteurs des enveloppes purifiées et radioactives et l’étude de la capacité de différents types de R5-CCKs à inhiber la liaison au corécepteur et l’infection. En outre, l’utilisation de récepteurs CCR5 mutants nous permettra d’évaluer l’hypothèse que l’acquisition de la résistance aux chimiokines est liée à l’utilisation de domaines différents du corécepteur. Nous évaluerons si ce processus s’accompagne aussi de modifications des propriétés fonctionnelles des gp120 . 

EXPERIENCE PROFESSIONNELLE ET QUALIFICATIONS : 

Le candidat devra avoir une forte expérience dans les domaines de la biologie et de la virologie moléculaires. Des connaissances dans le domaine de la pharmacologie des récepteurs couplés aux protéines G seraient un plus. Le candidat devra être très motivé, enthousiaste, savoir travailler de manière autonome mais également être doué d’un fort esprit d’équipe et de qualités relationnelles. 

Contact

Un CV, incluant les domaines de recherche, les techniques expérimentales connues et une liste de publications, et les coordonnées de référents sont à envoyer à : Dr Bernard Lagane (lagane@pasteur.fr ) or Dr Fernando Arenzana-Seisdedos (farenzan@pasteur.fr ), 

Institut Pasteur
Unité de Pathogénie Virale
28 rue du docteur Roux
75724 Paris cedex 15

Description

A post-doctoral position is available to study a new model of viral evolution leading to the emergence of epidemic and pathogenic vaccine-derived poliovirus strains through the recombination between vaccine poliovirus strains and coxsackie viruses. The project (ANR) includes the analysis of enterovirus isolates collected in the field (molecular epidemiology), the rational design of strains using genetic engineering technics and the characterization of recombinant viruses. An animal model is available to assess strains pathogenicity. Collaboration with laboratories of the Pasteur Institute International Network and possible short term periods abroad are envisaged.

Recent publications on the subject:

- Jegouic et al.. (2009). Recombination between polioviruses and co-circulating coxsackie A viruses: role in the emergence of pathogenic vaccine-derived polioviruses. PLoS Pathog 5(5), e1000412.

- Rakoto-Andrianarivelo et al., (2007). Co-circulation and evolution of polioviruses and species C enteroviruses in a district of Madagascar. PLoS Pathog 3(12), e191.

- Riquet et al.. (2008). Impact of exogenous sequences on the characteristics of an epidemic type 2 recombinant vaccine-derived poliovirus. J Virol 82(17), 8927-32. 

Contact

To apply, send a full C.V., a brief description of previous works (2 pages max.), the names and addresses of two referees to: 

Francis DELPEYROUX, 
PhD WHO Collaborating Centre of Research on Enteroviruses & Viral Vaccines. 
Biology of Enteric Viruses, 
Inserm U994, 
Institut Pasteur, 
75 724 Paris-cedex 15 - France 
e-mail: francis.delpeyroux@pasteur.fr
Tel: (33) 1 40 61 33 22

Description

Contact

Please, send a letter of intent along with a complete CV including a list of publications and the contact details of three referees to:

Dr. Oliver Bischof
Institut Pasteur
Unité Nuclear Organization and Oncogenesis; INSERM U993
25-28, rue du Docteur Roux
75724 Paris Cedex 15
France

Tel. 33 / 1 40 61 33 07
Fax 33 / 1 45 68 89 43
obischof@pasteur.fr

Description

Three year post-doc position available at the Institut Pasteur, Paris (Molecular Retrovirology Unit) 

The research fellow will carry out research on the APOBEC3 cytidine deaminases and the replication of retroviruses and DNA viruses with particular attention to the cell biology of the deaminases.

Candidates should hold a recent PhD and have experience in molecular and cell biology. The three year position is available as of now. Fluency in English is essential, while a knowledge of French would be useful but is not required.

Contact

A CV and names and addresses of two referees should be submitted by email to Pr. Simon Wain-Hobson (simon.wain-hobson@pasteur.fr)

Description

Macrophages are major targets of human immunodeficiency virus type 1 (HIV-1) infection. We have previously shown that the induction of the CDK inhibitor p21Waf-1/Cip1 by FcγR-aggregation and other stimuli inhibits the reverse transcription and integration of HIV-1 and related primate lentiviruses in human macrophages (1-3). In continuation with these studies we will now investigate the molecular mechanism by which p21 inhibits retroviral replication.

The candidate is expected to have a strong background in molecular biology and virology. Candidates with practical experience in biochemical analysis of protein interactions and RNA biochemistry will receive strong consideration. The successful candidate will be required to work in the BSL3 facility with competent HIV-1. 

The position is expected to start August 2010, for a 2-years period.

Candidates will join the research team “Factors of natural resistance to HIV-1 infection” of Dr. Gianfranco Pancino, in the Regulation of Retroviral Infections Unit (Director: Prof. Françoise Barré-Sinoussi, Nobel Prize in Medicine 2008)
http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-departments/virology/regulation-of-retroviral-infections/regulation-of-retroviral-infections.

Publications relative to the subject :
1. Perez-Bercoff D, et al. Fcγ receptor-mediated suppression of human immunodeficiency virus type 1 replication in primary human macrophages. J Virol. 2003 ;77:4081-94.
2. David, A, et al. The Engagement of Activating Fcγ Receptors Inhibits Primate Lentivirus Replication in Human Macrophages. J. Immunol., 2006, 177:6291-300.
3. Bergamaschi A, et al. The CDK inhibitor p21Cip1/WAF1 is induced by Fc{gamma}R activation and restricts the replication of HIV-1 and related primate lentiviruses in human macrophages. J Virol. 2009 Dec;83(23):12253-65. Epub 2009 Sep 16.
4. Bergamaschi A, et al. The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A-DDB1 DCAF1 ubiquitin ligase to overcome a postentry block in macrophage infection. J Virol. 2009 May;83(10):4854-60. Epub 2009 Mar 4.

Contact

Applications should include a CV, a letter of interest, and contact information of two referees, and submitted by e-mail to: Gianfranco Pancino

Description

Contact

Enquiries and applications (CV and references) may be made to

Sandrine Etienne-Manneville
Cell Polarity and Migration Group
sandrine.etienne-manneville@pasteur.fr
Tel : 01 40 61 39 05

Description

Contact

Un dossier de candidature sous la forme d’un CV, une liste de publication, un bref résumé de l’activité de recherche (4 pages max) ainsi que les coordonnées de deux personnes référentes doit être envoyés par Email à :

Dr Rémi Fronzes
Groupe de recherche G5 “Biologie structurale de la sécrétion bactérienne » Département de Biologie Structurale et Chimie.
Institut Pasteur,
25-28 rue du Docteur Roux
75724 Paris Cedex 15
France

Tel: 00 44 20 7631 6864

Email : r.fronzes@mail.cryst.bbk.ac.uk

Date limite : 1er décembre 2009.

Description

Applications are invited for a Post-Doctoral Research Assistant at the Oncogenesis and Molecular Virology Unit at Institut Pasteur, Paris, France.

The hepatitis B virus (HBV) is a small DNA virus that induces acute and chronic liver diseases, and represents a major risk factor for the development of hepatocellular carcinoma. An important step in the regulation of HBV replication is the transcription of pregenomic RNA from covalently closed circular DNA (cccDNA) organized in a chromatin-like structure.

The current project of the laboratory is to further understand how epigenetic modifications regulate transcription of this minichromosome at the different stages of viral infection. We will also investigate whether the transcriptional regulatory protein HBx is involved in such modifications through the recruitment of its cellular partners such as histone acetyltransferases (HATs).

Candidates should have a PhD and should have a proven laboratory track record in all aspects of virology, molecular biology and cell culture. The starting date will be decided in agreement with the selected candidate. The appointment will be financed by a grant from INCA.

Contact

Applications in the form of a CV, publication list and contact details of two referees, should be sent to :

Dr Christine Neuveut
Oncogenese et Virologie moleculaire / U 579 INSERM
Batiment Lwoff
Institut Pasteur
28 rue du Dr roux
75015 Paris
France

Tel. 33 / 1 45 68 88 51
Fax 33 / 1 45 68 89 43
email : christine.neuveut@pasteur.fr

Description

A postdoctoral position is available in the National Reference Centre for Borrelia, Mo-lecular Genetics of Bunyaviruses Unit, in Institut Pasteur, Paris, France.
This research opportunity is to identify, in the European tick-pathogen system (Ixodes ricinus- Borrelia burgdorferi sensu lato), protein interactions of benefit to the tick, the bacterium, or both. Briefly, this main objective will by addressed by complementary peptidomic, proteomic approaches and functional assays, plus the assessment of the effects on virulence and dissemination of European Borrelia strains in an animal model of Lyme borreliosis.

The position is supported by an Institut Pasteur funding guaranteed for at least two years as part of a “Transversal Research Program”. The candidate should have a Ph.D. in a relevant field (e.g. Molecular microbiology or entomology, Genetics, Bio-chemistry, Biology, etc.), and strong experience and documented ability in molecular biology and biochemistry. Applicants should submit by e-mail a letter of motivation and a CV, a summary of research activities (4 pages max) and two letters of professional references.

Contact

Dr Murielle Cornet
Centre National de Référence des Borrelia
Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur
25, rue du Dr Roux
75724 Paris Cedex 15, France
Tel 33 1 45 68 83 37
e mail : muriel.cornet@pasteur.fr

Or Valérie Choumet
Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur
25, rue du Dr Roux
75724 Paris Cedex 15, France
Tel 33 1 45 68 86 30
e mail : valerie.choumet@pasteur.fr