Multiple Roles of RNAs
Dr Marie-Christine Maurel
Université Pierre et Marie Curie
Dr Gwenaël Badis-Bréard,
Macromolecular Interaction Genetics,
Some years ago, the RNA world was divided in two categories: the protein coding RNA (mRNAs) and the non-coding RNAs such as tRNAs, snRNAs, snoRNAs and rRNAs. Several important concepts and discoveries such as splicing, catalytic RNA have pointed out the major role of RNA in gene expression. Recently, the discovery of novel classes of RNA like siRNAs, miRNAs and RNA interference (RNAi) shed light on novel major roles of RNA. It revealed a crucial role for RNA in many regulations of gene expression such as transcriptional and post-transcriptional controls. More recently, deep-sequencing and high-resolution microarrays revealed the existence of a highly complex population of non-coding transcripts. It is now clear that a plethora of stable and unstable RNAs, generated by a pervasive transcription, exists. Many questions remain open about the regulation and function and molecular mechanisms involving these non-coding RNAs.
Lectures are focused on the multiple roles of RNAs and specific technologies related to RNA. This supplements the " RNA Symposium ", the last day of the course, which takes place at the University Pierre et Marie Curie (Place Jussieu, Paris 5ème).
The aim of the practical course is to quantify nuclear RNA populations in mutant conditions, such as degradation mutant, in comparison with a wild type strain using qRT-PCR and Northern Blot combined to the use of microarrays. Here, we will use a strain deleted for RRP6, coding a nuclear 3’-5’ exoribonuclease component of the nuclear exosome, which is specifically involved in nuclear RNA degradation. The quantification of nuclear RNAs in mutant condition in comparison with wild type condition will be performed. This exploratory methodology could identify nuclear coding and non-coding RNAs and reveal novel specificities for some genes.
2012-2013 program can be downloaded to get more details about the course organization.
Certain lectures and laboratory works can change every year, however, aim and technics are maintained from year to year.
A good knowledge in genetic, molecular biology and biochemistry (level Master 2) is a prerequisite for admission to this course by the course commitee.
Full-time, except Saturdays, from february 17th, to february 28th, 2014
Number of students: 20
Closing-date for applications: September 1, 2013 (online registration)
The Institut Pasteur Diploma will be awarded to students having successfully completed the final examination,
Credits for Masters (second year) from Université Pierre et Marie Curie, for students registered at this university (masters),
Doctoral School Modules.
Members of the Course Commitee:
Y. Andéol (Université P. et M. Curie), G. Badis-Bréard (Institut Pasteur), C. Branlant (Université Nancy), M.-C. Daugeron* (Université Paris-Sud 11), M. Fromont-Racine (Institut Pasteur), M-C. Maurel (Université P. et M. Curie) ,C. Parsot (Institut Pasteur), C. Torchet (Université P. et M. Curie), A. Zider (Université Paris-Diderot P7)
* Representative of Universities