Multiple Roles of RNAs

Directors : Pr Yannick Andéol Université Pierre et Marie Curie-Paris 6 Dr Micheline Fromont-Racine Macromolecular Interaction Unit, Institut Pasteur

This course will be taught in French. However, the protocols book will be written in English
and some presentations will be in English.

This course is devoted to the exploration of the various roles that RNAs molecules are able to play into the gene expression and to the analysis of their regulations. Several lecture will be focused on the multiple roles of RNAs.
The objectives of the practical course are to study some steps of mRNA regulation such as export from the nucleus or stability. To this aim, the quantification of cytoplasmic and nuclear mRNAs in different mutant conditions will be performed at a genomic scale.
Practical course will consist of:
1) purification of ribonucleoproteic complexes;
2) extraction of associated RNAs;
3) hybridization onto micro arrays;
4) analysis of data after scanning;
5)validation of the results by classical approaches such as qRT-PCR.


* Full-time, except Saturdays, from February 16, to 27, 2009 (examinations included)
* Number of students: 20
* Closing-date for applications: October 15, 2008

Diplomas:

The Institut Pasteur Diploma will be awarded to students having successfully completed the final examination,

Credits for Masters (second year) from Université Pierre et Marie Curie-Paris 6, for students registered at this university (masters),

Members of the Teaching Committee:
Y. Andeol (Université P. et M. Curie-Paris 6), C. Branlant (Université Nancy), E. Coeffier-Vicart (Institut Pasteur), M.-C. Daugeron* (Université Paris-Sud 11), M. Fromont-Racine (Institut Pasteur), M.-C. Maurel* (Université P. et M. Curie-Paris 6), D. Morello (Université P. Sabatier, Toulouse), O. Ozier-Kalogeropoulos (Institut Pasteur/ Université P. et M. Curie-Paris 6), P. Régnier* (Université D. Diderot-Paris 7) I. Saint-Girons (Institut Pasteur), C. Torchet (Université P. et M. Curie-Paris 6)

* University Representatives

Lecturers: