Advanced Immunology
| Directors : Dr Andrès Alcover Lymphocyte Cell Biology Unit, Institut Pasteur Pr Antonio Freitas Lymphocyte Population Biology Unit, Institut Pasteur Head of Studies : Dr François Huetz Antiviral Cell Immunology Unit, Institut Pasteur |
Candidates must have good knowledge of Biochemistry, Molecular Biology and Genetics as well as a first year Master level, or equivalent in Immunology.
This course will be taught in French and in English.
The lecture program focuses on advanced studies of the main themes in cellular and molecular Immunology: molecular basis of antigen recognition by B and T cells, development of the immune system, repertoire selection and auto-immunity, intracellular communication, immune response, experimental immunotherapy and innate immunity.
The practical course involves the characterization of two functions of the AID protein (Activation Induced Cytidine Deaminase) in B cells: isotypic switch and somatic hypermutation. The role of AID in the switch will be studied in B cells cultured in the presence of LPS and IL4, by FACS analysis and ELISA. The role of AID in affinity maturation will be studied in cell lines transfected with a reporter GFP vector. Mutated cells will be detected by fluorescence and sequence analysis of BCR genes.
One week of group seminars supervised by two senior scientists.
* Full-time, Saturdays included, November 3, to December 20, 2008 and January 5, to 9, 2009 (Written examination on January 5, 2009 – oral examination if necessary, on January 9, 2009)
* Number of students: 24
* Closing-date for applications: June 18, 2008
Diplomas:
The Institut Pasteur Diploma will be awarded to students having successfully completed the final examination,
This course is run in agreement with the Université Pierre et Marie Curie-Paris 6 and Denis Diderot-Paris 7 and a University Diploma may also be granted,
Credits for Masters (second year) from Universités René Descartes-Paris5, Pierre et Marie Curie-Paris 6 and Denis Diderot-Paris 7, for students registered at this universities (masters),
Doctoral School Modules.
Members of the Teaching Committee:
J.-F. Bach* (Université R. Descartes-Paris 5), P.-A. Cazenave* (institut Pasteur/ Université P. et M. Curie-Paris 6), E. Coeffier-Vicart (Institut Pasteur), A. Freitas (Institut Pasteur), C. Fridman (Université R. Descartes-Paris 5), F. Huetz (Institut Pasteur), O. Ozier-Kalogeropoulos (Institut Pasteur/ Université P. et M. Curie-Paris 6), I. Saint Girons (Institut Pasteur), M. Seman* (Université D. Diderot-Paris 7).
* University Representatives
Lecturers:
L. Abel, G. Anderson, B. Autran, N. van Baren, G. Bismuth, C. Boitard, P. Bousso, S. Coddold, F. Colucci, R. Cornall, A. Cumano, M. Daha, M. Daeron, J. di Santo, A. Fischer, R. Forster, T. de France, A. Freitas, P. Golstein, A. Hayday, M. Heikenwaelder, L. Klein, G. Kraal, R. Kroczek, A. Lanzavecchia, C. Leclerc, F. Lemonnier, J. Louis, R. Mac Donald, L. du Pasquier, F. Radtke, J.-M. Reichhart, W. Reith, B. Rocha, H.-R. Rodewald, B. Stockinger, J.-M. Thiercy et J.-C. Weill.