Tuberculosis : from molecular diagnosis and drug susceptibility testing to treatment
Pr Brigitte Gicquel
Mycobacterial Genetics Unit
Head of Studies :
Dr Sara Eyangoh
Centre Pasteur (Yaounde - Cameroon)
This course is intended for medical doctors, veterinarians, pharmacists and research scientists wishing to acquire an up to date knowledge on tuberculosis and in the practice of molecular methods for diagnosis drug susceptibility testing and epidemiology.
The language for the lectures and the practical training worshop will be English. For people who might need it, help in French will be provided during the practical training workshop.
This full-time course is composed of lectures given by international scientists and a practical training workshop.
Lectures will cover the global epidemiology of tuberculosis (TB); molecular epidemiology and evolution of Mycobacterium tuberculosis family strains; host-pathogen interactions; immune responses during tuberculosis infection and disease; HIV co-infection; mechanisms of antibiotic resistance, multidrug-resistance (MDR), and extensive drug resistance (XDR); concepts and applications of rapid diagnosis and drug susceptibility tests (DST) in different settings; evaluation of the different methods using predictive values according to TB prevalence; anti-TB treatments including MDR-TB and XDR-TB; diagnosis of TB infection; BCG vaccination, and new vaccine candidates.
The practical training workshop will provide training for the different commercial tests for rapid diagnosis and DST using nucleic acid-based technologies: GenoType® MTBDRplus and MTBDRsl assay (HAIN Lifescience GmbH, Germany), and the GeneXpert tests (Cepheid, Sunnyvale, CA, USA). Antibiotic gene targets will be sequenced to identify antibiotic resistances (sequencing platforms and free softwares for DNA sequences analysis will be used). Several molecular epidemiology techniques will be compared: spoligotyping, MIRU/VNTR typing, and single nucleotide polymorphisms (SNPs) genotyping. We will work with bacterial inactivated cultures but also directly with inactivated clinical samples, without previous culturing.
Full-time, except Saturdays and Sundays, from June 17th, to 28th, 2013 (examinations included)
Number of students: 20
Closing-date for applications: Fébruary 15, 2013 (online registration)
The Institut Pasteur Diploma will be awarded to students having successfully completed the final examination,
This course is run in agreement with the Université Paris-Diderot and a University Diploma may be delivered by this university ; validation of the course is pending.,
ECTS, exclusively for students registered in the Master’s training in Public Health - Pasteur/Cnam of Public Health
Members of the Teaching Committee:
R. Brosch (Institut Pasteur), E. Coeffier (Institut Pasteur), S. Eyangoh (Yaounde Cameroon), A. Fontanet (Institut Pasteur), B. Gicquel (Institut Pasteur), J.-L. Herrmann (Hôpital Saint-Louis, Paris), C. Pierre-Audigier (Institut Pasteur), A. Trebucq (UICTMR Paris), C. Parsot (Institut Pasteur).
Christopher Dye, Arnaud Trebucq, Sebastien Gagneux, Philippe Supply, Olivier Neyrolles, Steffen Stenger, Catherine Pierre-Audigier, Brigitte Gicquel, Roland Brosch, Sara Eyangoh, Amine Namouchi, Muriel Vray, Françoise Mascart, Marie-Line Bonnet, Vincent Jarlier, Laurent Abel, Thierry Wirth.