Research / Viral Toxins

Viral toxins - Marie Flamand

Biological study of secreted dengue virus nonstructural protein NS1
Flavivirus NS1 is involved in the intracellular stages of virus replication. It also circulates in the extracellular medium although its related function remains unknown. We have developed a dengue diagnostic kit based on the NS1 antigen capture by ELISA that has become a reference in the field since its commercialization by BioRad in 2006. We are currently pursuing the functional characterization of the secreted form of dengue virus NS1 in an attempt to specify its contribution to fatal hemorrhagic fevers.



Balakrishnan T, Bela-Ong DB, Toh YX, Flamand M, Devi S, Koh MB, Hibberd ML, Ooi EE, Low JG, Leo YS, Gu F, Fink K. Dengue virus activates polyreactive, natural IgG B cells after primary and secondary infection. PLoS One. 2011;6(12):e29430.

Chuansumrit A, Chaiyaratana W, Tangnararatchakit K, Yoksan S, Flamand M, Sakuntabhai A. Dengue nonstructural protein 1 antigen in the urine as a rapid and convenient diagnostic test during the febrile stage in patients with dengue infection. Diagn Microbiol Infect Dis. 2011 Dec;71(4):467-9.

Gutsche I, Coulibaly F, Voss JE, Salmon J, d'Alayer J, Ermonval M, Larquet E, Charneau P, Krey T, Mégret F, Guittet E, Rey FA, Flamand M. Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein. Proc Natl Acad Sci U S A. 2011 May 10;108(19):8003-8.


Ermonval M., Baudry A., Baychelier F., Pradines E., Pietri M., Oda K., Schneider B., Mouillet-Richard S., Launay J.M., Kellermann O.The cellular prion protein interacts with the tissue non-specific alkaline phosphatase in membrane microdomains of bioaminergic neuronal cells. PLoS One. 4(8):e6497.

Ermonval, M., Petit, D., Le Duc, A., Kellermann, O. & Gallet, P. F. Glycosylation-related genes are variably expressed depending on the differentiation state of a bioaminergic neuronal cell line: implication for the cellular prion protein. Glycoconj J. 26(4):477-489.


Schul W., Liu W., Xu H.Y., Flamand M., Vasudevan S.G. A dengue Fever viremia model in mice shows reduction in viral replication and suppression of the inflammatory response after treatment with antiviral drugs. J Infect Dis. 195(5):665-74.


Dussart P., Labeau B., Lagathu G., Louis P., Nunes M.R., Rodrigues S.G., Storck-Herrmann C., Cesaire R., Morvan J., Flamand M., Baril L. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 13(11):1185-9.

Alcon-LePoder S., Sivard P., Drouet M.-T., Talarmin A., Rice C., Flamand M. Secretion of flaviviral non-structural protein NS1: from diagnosis to pathogenesis. In, « New Treatment Strategies for Dengue and Other Flaviviral Diseases » Novartis Foundation Eds., Wiley pp 233-250.