Publications de 1997 à 2005

Publications 2008

Regulation of glutamate metabolism by protein kinases in mycobacteria.

O’Hare HM, Durán R, Cerveñansky C, Bellinzoni M, Wehenkel AM, Pritsch O, Obal G, Baumgartner J, Vialaret J, Johnsson K, Alzari PM.

Mol Microbiol. 2008 Dec;70(6):1408-23.

Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD.

Krehenbrink M, Chami M, Guilvout I, Alzari PM, Pécorari F, Pugsley AP.

J Mol Biol. 2008 Nov 28;383(5):1058-68.

Rising standards for tuberculosis drug development.

Balganesh TS, Alzari PM, Cole ST.

Trends Pharmacol Sci. 2008 Nov;29(11):576-81.

Structural insights into sialic acid enzymology.

Buschiazzo A, Alzari PM.

Curr Opin Chem Biol. 2008 Oct;12(5):565-72.


Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis.

Damager I, Buchini S, Amaya MF, Buschiazzo A, Alzari PM, Frasch AC, Watts A, Withers SG.

Biochemistry. 2008 Mar 18;47(11):3507-12.

Crystal structure of a monoclonal antibody directed against an antigenic determinant common to Ogawa and Inaba serotypes of Vibrio cholerae O1.

Ahmed F, André-Leroux G, Haouz A, Boutonnier A, Delepierre M, Qadri F, Nato F, Fournier JM, Alzari PM.

Proteins. 2008 Jan 1;70(1):284-8.


Mycobacterial Ser/Thr protein kinases and phosphatases: physiological roles and therapeutic potential.

Wehenkel A, Bellinzoni M, Graña M, Duran R, Villarino A, Fernandez P, Andre-Leroux G, England P, Takiff H, Cerveñansky C, Cole ST, Alzari PM.

Biochim Biophys Acta. 2008 Jan;1784(1):193-202.

Publications 2007

Structural and binding studies of the three-metal center in two mycobacterial PPM Ser/Thr protein phosphatases.

Wehenkel A, Bellinzoni M, Schaeffer F, Villarino A, Alzari PM.

J Mol Biol. 2007 Dec 7;374(4):890-8.

Towards new tuberculosis drugs.

Cole ST, Alzari PM.

Biochem Soc Trans. 2007 Nov;35(Pt 5):1321-4.


Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD.

Mouratou B, Schaeffer F, Guilvout I, Tello-Manigne D, Pugsley AP, Alzari PM, Pecorari F.

Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17983-8.


The crystal structure of Trypanosoma cruzi arginine kinase.

Fernandez P, Haouz A, Pereira CA, Aguilar C, Alzari PM.

Proteins. 2007 Oct 1;69(1):209-12.

The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria.

Graña M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM.

Protein Sci. 2007 Sep;16(9):1896-904.

Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis.

Stadthagen G, Sambou T, Guerin M, Barilone N, Boudou F, Korduláková J, Charles P, Alzari PM, Lemassu A, Daffé M, Puzo G, Gicquel B, Rivière M, Jackson M.

J Biol Chem. 2007 Sep 14;282(37):27270-6.

Insights into the catalytic mechanism of PPM Ser/Thr phosphatases from the atomic resolution structures of a mycobacterial enzyme.

Bellinzoni M, Wehenkel A, Shepard W, Alzari PM.

Structure. 2007 Jul;15(7):863-72.


Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives -- a screening based on affinity for the UMP-CMP binding site.

Topalis D, Kumamoto H, Amaya Velasco MF, Dugué L, Haouz A, Alexandre JA, Gallois-Montbrun S, Alzari PM, Pochet S, Agrofoglio LA, Deville-Bonne D.

FEBS J. 2007 Jul;274(14):3704-14.


Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria.

Guerin ME, Kordulakova J, Schaeffer F, Svetlikova Z, Buschiazzo A, Giganti D, Gicquel B, Mikusova K, Jackson M, Alzari PM.

J Biol Chem. 2007 Jul 13;282(28):20705-14.

Benzoic acid and pyridine derivatives as inhibitors of Trypanosoma cruzi trans-sialidase.

Neres J, Bonnet P, Edwards PN, Kotian PL, Buschiazzo A, Alzari PM, Bryce RA, Douglas KT.

Bioorg Med Chem. 2007 Mar 1;15(5):2106-19.

The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria.

Shepard W, Haouz A, Graña M, Buschiazzo A, Betton JM, Cole ST, Alzari PM.

J Bacteriol. 2007 Mar;189(5):1899-904.

Insights into the inter-ring plasticity of caseinolytic proteases from the X-ray structure of Mycobacterium tuberculosis ClpP1.

Ingvarsson H, Maté MJ, Högbom M, Portnoï D, Benaroudj N, Alzari PM, Ortiz-Lombardía M, Unge T.

Acta Crystallogr D Biol Crystallogr. 2007 Feb;63(Pt 2):249-59.

Towards the molecular understanding of glycogen elongation by amylosucrase.

Albenne C, Skov LK, Tran V, Gajhede M, Monsan P, Remaud-Siméon M, André-Leroux G.

Proteins. 2007 Jan 1;66(1):118-26.


Publications 2006

Peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons.

Pehar M, Vargas MR, Robinson KM, Cassina P, England P, Beckman JS, Alzari PM, Barbeito L.

Free Radic Biol Med. 2006 Dec 1;41(11):1632-44.



Allosteric activation of the protein kinase PDK1 with low molecular weight compounds.

Engel M, Hindie V, Lopez-Garcia LA, Stroba A, Schaeffer F, Adrian I, Imig J, Idrissova L, Nastainczyk W, Zeuzem S, Alzari PM, Hartmann RW, Piiper A, Biondi RM.

EMBO J. 2006 Nov 29;25(23):5469-80.


Structure of armadillo ACBP: a new member of the acyl-CoA-binding protein family.

Costabel MD, Ermácora MR, Santomé JA, Alzari PM, Guérin DM.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):958-61.


Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens.

Fogg MJ, Alzari PM, Bahar M, Bertini I, Betton JM, Burmeister WP, Cambillau C, Canard B, Corrondo MA, Coll M, Daenke S, Dym O, Egloff MP, Enguita FJ, Geerlof A, Haouz A, Jones TA, Ma Q, Manicka SN, Migliardi M, Nordlund P, Owens RJ, Peleg Y, Schneider G, Schnell R, Stuart DI, Tarbouriech N, Unge T, Wilkinson AJ, Wilmanns M, Wilson KS, Zimhony O, Grimes JM.

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1196-207.



SPINE bioinformatics and data-management aspects of high-throughput structural biology.

Albeck S, Alzari PM, Andreini C, Banci L, Berry IM, Bertini I, Cambillau C, Canard B, Carter L, Cohen SX, Diprose JM, Dym O, Esnouf RM, Felder C, Ferron F, Guillemot F, Hamer R, Ben Jelloul M, Laskowski RA, Laurent T, Longhi S, Lopez R, Luchinat C, Malet H, Mochel T, Morris RJ, Moulinier L, Oinn T, Pajon A, Peleg Y, Perrakis A, Poch O, Prilusky J, Rachedi A, Ripp R, Rosato A, Silman I, Stuart DI, Sussman JL, Thierry JC, Thompson JD, Thornton JM, Unger T, Vaughan B, Vranken W, Watson JD, Whamond G, Henrick K.

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1184-95.


Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

Alzari PM, Berglund H, Berrow NS, Blagova E, Busso D, Cambillau C, Campanacci V, Christodoulou E, Eiler S, Fogg MJ, Folkers G, Geerlof A, Hart D, Haouz A, Herman MD, Macieira S, Nordlund P, Perrakis A, Quevillon-Cheruel S, Tarandeau F, van Tilbeurgh H, Unger T, Luna-Vargas MP, Velarde M, Willmanns M, Owens RJ.

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1103-13.



The Ser/Thr protein kinase PknB is essential for sustaining mycobacterial growth.

Fernandez P, Saint-Joanis B, Barilone N, Jackson M, Gicquel B, Cole ST, Alzari PM.

J Bacteriol. 2006 Nov;188(22):7778-84.



Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.

Schujman GE, Guerin M, Buschiazzo A, Schaeffer F, Llarrull LI, Reh G, Vila AJ, Alzari PM, de Mendoza D.

EMBO J. 2006 Sep 6;25(17):4074-83.



Continuous fluorimetric assay for high-throughput screening of inhibitors of trans-sialidase from Trypanosoma cruzi.

Neres J, Buschiazzo A, Alzari PM, Walsh L, Douglas KT.

Anal Biochem. 2006 Oct 15;357(2):302-4.



The structure of PknB in complex with mitoxantrone, an ATP-competitive inhibitor, suggests a mode of protein kinase regulation in mycobacteria.

Wehenkel A, Fernandez P, Bellinzoni M, Catherinot V, Barilone N, Labesse G, Jackson M, Alzari PM.

FEBS Lett. 2006 May 29;580(13):3018-22.




The crystal structure of Mycobacterium tuberculosis adenylate kinase in complex with two molecules of ADP and Mg2+ supports an associative mechanism for phosphoryl transfer.

Bellinzoni M, Haouz A, Graña M, Munier-Lehmann H, Shepard W, Alzari PM.

Protein Sci. 2006 Jun;15(6):1489-93.

Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis.

Guerin ME, Buschiazzo A, Korduláková J, Jackson M, Alzari PM.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt 5):518-20.

Crystal structure, catalytic mechanism, and mitogenic properties of Trypanosoma cruzi proline racemase.

Buschiazzo A, Goytia M, Schaeffer F, Degrave W, Shepard W, Grégoire C, Chamond N, Cosson A, Berneman A, Coatnoan N, Alzari PM, Minoprio P.

Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1705-10.

Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase.

Watts AG, Oppezzo P, Withers SG, Alzari PM, Buschiazzo A.

J Biol Chem. 2006 Feb 17;281(7):4149-55.



Publications 2005

dentification of the critical residues involved in peptidoglycan detection by Nod1.

Girardin SE, Jéhanno M, Mengin-Lecreulx D, Sansonetti PJ, Alzari PM, Philpott DJ.

J Biol Chem. 2005 Nov 18;280(46):38648-56.

Calorimetric and crystallographic analysis of the oligomeric structure of Escherichia coli GMP kinase.

Hible G, Renault L, Schaeffer F, Christova P, Zoe Radulescu A, Evrin C, Gilles AM, Cherfils J.

J Mol Biol. 2005 Oct 7;352(5):1044-59. 

Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases.

Durán R, Villarino A, Bellinzoni M, Wehenkel A, Fernandez P, Boitel B, Cole ST, Alzari PM, Cerveñansky C.

Biochem Biophys Res Commun. 2005 Aug 5;333(3):858-67.

Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions.

Villarino A, Duran R, Wehenkel A, Fernandez P, England P, Brodin P, Cole ST, Zimny-Arndt U, Jungblut PR, Cerveñansky C, Alzari PM.

J Mol Biol. 2005 Jul 29;350(5):953-63.

Structure and mechanism of the alkyl hydroperoxidase AhpC, a key element of the Mycobacterium tuberculosis defense system against oxidative stress.

Guimarães BG, Souchon H, Honoré N, Saint-Joanis B, Brosch R, Shepard W, Cole ST, Alzari PM.

J Biol Chem. 2005 Jul 8;280(27):25735-42.

Action pattern of Fusarium moniliforme endopolygalacturonase towards pectin fragments: Comprehension and prediction.

André-Leroux G, Tessier D, Bonnin E.

Biochim Biophys Acta. 2005 May 20;1749(1):53-64.

Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis.

Guerin ME, Buschiazzo A, Korduláková J, Jackson M, Alzari PM.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt 5):518-20.


A sialidase mutant displaying trans-sialidase activity.

Paris G, Ratier L, Amaya MF, Nguyen T, Alzari PM, Frasch AC.

J Mol Biol. 2005 Jan 28;345(4):923-34.

I Microbiology. TB--a new target, a new drug.

Cole ST, Alzari PM.

Science. 2005 Jan 14;307(5707):214-5.

Normal mode analysis suggests a quaternary twist model for the nicotinic receptor gating mechanism.

Taly A, Delarue M, Grutter T, Nilges M, Le Novère N, Corringer PJ, Changeux JP.

Biophys J. 2005 Jun;88(6):3954-65.

Refinement of docked protein-ligand and protein-DNA structures using low frequency normal mode amplitude optimization.

Lindahl E, Delarue M.

Nucleic Acids Res. 2005 Aug 8;33(14):4496-506.

Publications 2004

Alzari PM. First structural glimpse at a bacterial Ser/Thr protein phosphatase. Structure (Camb). 2004 Nov;12(11):1923-4.

Buschiazzo A, Ugalde JE, Guerin ME, Shepard W, Ugalde RA, Alzari PM. Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation. EMBO J. 2004 Aug 18;23(16):3196-205. 

Freire T, Fernandez C, Chalar C, Maizels RM, Alzari P, Osinaga E, Robello C. Characterization of a UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase with an unusual lectin domain from the platyhelminth parasite Echinococcus granulosus. Biochem J. 2004 Sep 1;382(Pt 2):501-10.

Amaya MF, Watts AG, Damager I, Wehenkel A, Nguyen T, Buschiazzo A, Paris G, Frasch AC, Withers SG, Alzari PM. Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Structure (Camb). 2004 May;12(5):775-84.

Assari L, Bertrand T, Ferdinand J, Slavova-Azmanova N, Christensen M, Briozzo P, Schaeffer F, Craescu CT, Neuhard J, Barzu O, Gilles AM. Deciphering the function of an ORF: Salmonella enterica DeoM protein is a new mutarotase specific for deoxyribose. Protein Sci 2004; 13:1295-1303.

Delarue M, Dumas P. Enforcing large amplitude and collective movements in the refinement of macromolecular models with low resolution data. Proc Natl Acad Sci USA 2004; 101:6957-6961.

C. Albenne, L. Skov, O. Mirza,M. Gajhede, G. Feller, S. D’Amico, G. André, G. Potocki-Véronèse, B. van der Veen, P. Monsan and M. Remaud-Simeon Molecular Basis of the Amylose-like Polymer Formation Catalyzed by Neisseria polysaccharea Amylosucrase. J. Biol. Chem,Vol 279, pp726-734 (2004).

K. Bak-Jensen, G. André, T. Gottschalk, G. Paës, V. Tran and B. Svensson Roles of Tyrosine 105 and Threonine 212 at Outermost Subsites -6 and +4 in Substrate Specificity, Oligosaccharide Cleavage Pattern, and Multiple Binding Modes of Barley α-Amylase 1 J. Biol. Chem., Vol 279, pp10093-10102 (2004).

G. André and V. Tran Putative Implication of α-amylase Loop 7 in the Mechanism of Substrate Binding and Reaction Products Release. Biopolymers, Vol 75, pp 95-108 (2004).

A. Yiannikouris, G. André, A. Buléon, G. Jeminet, I. Canet, J. François, G. Bertin and JP. Jouany  Comprehensive Conformational Study of key Interactions Involved in Zearalenone Complexation in a -D Glucans. Biomacromolecules, Vol5, No6, pp 2176-2185 (2004).

Publications 2003

Ribard C, Rochet M, Labedan B, Daignan-Fornier B, Alzari P, Scazzocchio C, Oestreicher N. Sub-families of alpha/beta barrel enzymes: a new adenine deaminase family. J Mol Biol. 2003 Dec 12;334(5):1117-31.

Boitel B, Ortiz-Lombardia M, Duran R, Pompeo F, Cole ST, Cervenansky C, Alzari PM. PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis. Mol Microbiol. 2003 Sep;49(6):1493-508.

Watts AG, Damager I, Amaya ML, Buschiazzo A, Alzari P, Frasch AC, Withers SG. Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile. J Am Chem Soc. 2003 Jun 25;125(25):7532-3. No abstract available.

Guerin ME, Buschiazzo A, Ugalde JE, Ugalde RA, Alzari PM. Preliminary crystallographic studies of glycogen synthase from Agrobacterium tumefaciens. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):526-8. Epub 2003 Feb 21.

Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM. The crystal structure of a plant lectin in complex with the Tn antigen. FEBS Lett. 2003 Feb 11;536(1-3):106-10.

Ortiz-Lombardia M, Pompeo F, Boitel B, Alzari PM. Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis. J Biol Chem. 2003 Apr 11;278(15):13094-100. Epub 2003 Jan 27.

Amaya MF, Buschiazzo A, Nguyen T, Alzari PM. The high resolution structures of free and inhibitor-bound Trypanosoma rangeli sialidase and its comparison with T. cruzi trans-sialidase. J Mol Biol. 2003 Jan 24;325(4):773-84.

Haouz A, Munier-Lehmann H, van Callenbergh S, Froeyen M, Herdewijn P, Delarue M. Enzymatic and structural studies of inhibitors designed against thymidylate kinase from M. tuberculosis: insight into the phosphoryl transfer mechanism. J Biol Chem 2003; 278:4963-71.

Fioravanti E, Haouz A, Ursby T, Munier-Lehmann H, Delarue M, Bourgeois D. Crystal structure of several intermediates along the reaction pathway of M. tuberculosis thymidylate kinase.
J Mol Biol 2003; 327:1077-1092.

Publications 2002

Buschiazzo A, Amaya MF, Cremona ML, Frasch AC, Alzari PM. The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis. Mol Cell. 2002 Oct;10(4):757-68.

Mo F, Mathiesen RH, Alzari PM, Lescar J, Rasmussen B. Physical estimation of triplet phases from two new proteins. Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1780-6. Epub 2002 Sep 28.

Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS. Advantages of high-resolution phasing: MAD to atomic resolution. Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1433-41. Epub 2002 Aug 23.

Guimaraes BG, Souchon H, Lytle BL, David Wu JH, Alzari PM. The crystal structure and catalytic mechanism of cellobiohydrolase CelS, the major enzymatic component of the Clostridium thermocellum Cellulosome. J Mol Biol. 2002 Jul 12;320(3):587-96.

Montagna G, Cremona ML, Paris G, Amaya MF, Buschiazzo A, Alzari PM, Frasch AC. The trans-sialidase from the african trypanosome Trypanosoma brucei. Eur J Biochem. 2002 Jun;269(12):2941-50.

Smith SP, Beguin P, Alzari PM, Gehring K. 1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module. J Biomol NMR. 2002 May;23(1):73-4. No abstract available.

Carpena X, Guarne A, Ferrer JC, Alzari PM, Fita I, Loewen PC. Crystallization and preliminary X-ray analysis of the hydroperoxidase I C-terminal domain from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):853-5. Epub 2002 Apr 26.

Mate MJ, Ortiz-Lombardia M, Boitel B, Haouz A, Tello D, Susin SA, Penninger J, Kroemer G, Alzari PM. The crystal structure of the mouse apoptosis-inducing factor AIF. Nat Struct Biol. 2002 Jun;9(6):442-6.

Chernyak A, Kondo S, Wade TK, Meeks MD, Alzari PM, Fournier JM, Taylor RK, Kovac P, Wade WF. Induction of protective immunity by synthetic Vibrio cholerae hexasaccharide derived from V. cholerae O1 Ogawa lipopolysaccharide bound to a protein carrier. J Infect Dis. 2002 Apr 1;185(7):950-62. Epub 2002 Mar 19.

Mowat CG, Leys D, McLean KJ, Rivers SL, Richmond A, Munro AW, Ortiz Lombardia M, Alzari PM, Reid GA, Chapman SK, Walkinshaw MD. Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis. Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):704-5. Epub 2002 Mar 22.

Guerin DM, Lascombe MB, Costabel M, Souchon H, Lamzin V, Beguin P, Alzari PM. Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate. J Mol Biol. 2002 Mar 8;316(5):1061-9.

Miras I, Schaeffer F, Beguin P, Alzari PM. Mapping by site-directed mutagenesis of the region responsible for cohesin-dockerin interaction on the surface of the seventh cohesin domain of Clostridium thermocellum CipA. Biochemistry. 2002 Feb 19;41(7):2115-9.

Schaeffer F, Matuschek M, Guglielmi G, Miras I, Alzari PM, Beguin P. Duplicated dockerin subdomains of Clostridium thermocellum endoglucanase CelD bind to a cohesin domain of the scaffolding protein CipA with distinct thermodynamic parameters and a negative cooperativity. Biochemistry. 2002 Feb 19;41(7):2106-14.

Ursby T, Weik M, Fioravanti E, Delarue M, Goeldner M, Bourgeois D. Cryo-photoloysis of caged compounds: a technique for trapping intermediates in protein crystals. Acta Crystallogr D 2002; 58:607-614.

Manallack DT, Pitt WR, Herdewijn P, Balzarini de Clercq JE, Sanderson MR, Soh M, Wien F, Munier-Lehmann H, Haouz A, Delarue M. Database searching for thymidine and thymidylate kinase inhibitors using three-dimensional structure-based methods. Enzyme Inh Med Chem 2002; 17:167-174.

Saveanu C, Miron S, Borza T, Craescu CT, Labesse G, Gagyi C, Popescu A, Schaeffer F, Namane A, Laurent-Winter C, Barzu O, Gilles AM. Structural and nucleotide-binding properties of YajQ and YnaF, two Escherichia coli proteins of unknown function. Protein Sci 2002; 11:2551-2560.

Navaza, J., Alzari, PM. Automated molecular replacement. In Protein Structure: Determination, Analysis, and Applications for Drug Discovery, edited by D. Chasman.Dekker (2002).

Delarue M, Sanejouand YH. Simplified normal mode analysis of the open-closed transition in DNA-dependent polymerases. J Mol Biol 2002; 320:1011-1024.

Delarue M, Boulé JB, Lescar J, Expert-Bezançon N, Jourdan N, Rougeon F, Papanicolaou C. Crystal structures at 2.35 Angstrom resolution of a template independent DNA polymerase: murine terminal desoxynucleotidyltransferase. EMBO J 2002; 21:427-439.

Navaza J, Alzari PM. Molecular replacement and high throughput structure determination. Zeits Kristallogr 2002; 217:715-721.

Publications 2001

Paris, G., Cremona, M.L., Amaya, M.F., Buschiazzo, A., Giambiagi, S., Frasch, A.C.C., Alzari, P.M. Probing molecular function of trypanosomal sialidases : single point mutations can change substrate specificity and increase hydrolytic activity. Glycobiology,11 : 305-311

Pitcovsky, T.A., Mucci, J., Alvarez, P., Leguizamon, M.S., Burrone, O., Alzari, P.M., Frasch, A.C.C., Campetella, O. The epitope mapping of the trans-sialidase from Trypanosoma cruzi reveals the presence of several cross-reactive determinants. Infection & Immunity, 69 : 1869-1875

Li de la Sierra I, Munier-Lehmann H, Gilles AM, Barzu O, Delarue M. Crystal structure of Mycobacterium tuberculosis TMP kinase complexed with TMP at 1.95 Å resolution. J Mol Biol 2001; 311:87-10.

Publications 2000

Schaeffer, F. Energies and forces of biomacromolecular interactions: quantitative aspects. Microcalorimetry studies of biomolecular interactions. Cahier du C.N.R.S. Physique et Chimie du Vivant 2, 47-56

Pritsch, O., Magnac, C., Dumas, G., Bouvet, J.-P., Alzari, P.M., Dighiero, G. Can isotype switch modulate antigen binding affinity and influence clonal selection? European Journal of Immunology, 30 : 3387-3395

Oppezzo, P., Osinaga, E., Tello, D., Bay, S., Cantacuzène, D., Irigoin, F., Ferreira, A., Cayota, A., Alzari, P.M., Pritsch, O. Production and functional characterization of two mouse/human chimeric antibodies with specificity for the tumor-associated Tn antigen. Hybridoma, 19 : 229-239

Villeneuve, S., Souchon, H., Riottot, M.M., Mazie, J.C., Kovacs, P., Fournier, J.M., Alzari, P.M. The X-ray structure of an anti-carbohydrate antibody directed against Vibrio cholerae in complex with antigen. Proceedings of the National Academy of Sciences USA, 97 : 8433-8438

Lascombe, M.-B., Grégoire, C., Tavares, G.A., Poncet, P., Rosinski-Chupin, I., Rabillon, J., Goubran-Botros, H., Mazié, J.-C., David, B., Alzari, P.M. Crystal structure of the major horse allergen Equ c 1, a dimeric lipocalin with restricted IgE-reactive epitopes. Journal of Biological Chemistry, 275 : 21572-21577

Eckenberg, R., Rose, T., Moreau, J.-L., Weil, R., Gesbert, F., Dubois, S., Tello, D., Bossus, M., Gras, H., Tartar, A., Bertoglio, J., Chouaib, S., Goldberg, M., Jacques, Y., Alzari, P.M., Thèze, J. The first alpha-helix of interleukin IL-2 folds as a homotetramer, acts as an agonist of the IL-2 receptor beta-chain and induces lymphokine-activated killer cells. Journal of Experimental Medicine 191 : 529-540

Osinaga, E., Bay, S., Tello, D., Babino, A., Pritsch, O., Assemat, K., Cantacuzène, D., Nakada, H., Alzari, P.M. Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface plasmon resonance spectroscopy. FEBS Letters 469 : 24-28

Buschiazzo, A., Tavares, G.A., Campetella, O., Spinelli, S., Cremona, M.L., Paris, G., Amaya, M.F., Frasch, A.C.C., Alzari, P.M. Stuctural basis of sialyltransferase activity in Trypanosomal sialidases. EMBO Journal 19 : 16-24

Medeiros, A., Bianchi, S., Calvete, J.J., Balter, H., Bay, S., Robles, A., Cantacuzène, D., Nimtz, M., Alzari, P.M., Osinaga, E. Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds. European Journal of Biochemistry 267 : 1434-1440

Publications 1999

Barale, J.C., Blisnick, T., Fujioka, H., Alzari, P.M., Aikawa, M., Braun-Breton, C., Langsley, G. Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite-surface-protein 1-42 maturase. Proceedings of the National Academy of Sciences USA 96 : 6445-6450

Susin, S.A., Lorenzo, H.K., Zamzami, N., Marzo, I., Snow, B.E., Brothers, G.M., Mangion, J., Jacotot, E., Costantini, P., Loeffler, M., Larochette, N., Goodlett, D.R., Aebersold, R., Siderovski, D.P., Penninger, J.M., Kroemer, G. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397 : 441-446

Goldberg, M.E., Schaeffer, F., Guillou, Y., Djavadi-Ohaniance, L. Pseudo-native motifs in the noncovalent heme-apocytochrome C complex : evidence from antibody binding studies by enzyme-linked immunosorbent assay and microcalorimetry. Journal of Biological Chemistry 274 : 16052-16061

Marina, A., Alzari, P.M., Bravo, J., Uriarte, M., Barcelona, B., Fita, I., Rubio, V. Structure of carbamate kinase, the key enzyme for fermentative ATP production from arginine Protein Science 8 : 934-940

Saint-Joanis, B., Souchon, H., Wilming, M., Johnsson, K., Alzari, P.M., Cole, S.T. Use of site-directed mutagenesis to probe structure, function and isoniazid-activation by catalase-peroxidase, KatG, from Mycobacterium tuberculosis Biochemical Journal 338 : 753-760

Grégoire, C., Tavares, G.A., Lorenzo, H.K., Dandeu, J.P., David, B., Alzari, P.M. Crystallization and preliminary crystallographic analysis of the major horse allergen Equ c 1. Acta Crystallographica D55 : 880-882

Maté, M.J., Zamocky, M., Nykyri, L.M., Herzog, C., Alzari, P.M., Betzel, C., Koller, F., Fita, I. Structure of catalase A from Saccharomyces cerevisiae. Journal of Molecular Biology 286 : 135-149

Susin, S.A., Lorenzo, H.K., Zamzami, N, Marzo, I, Brenner, C., Larochette, N., Prevost, M.C., Alzari, P.M., Kroemer, G. Mitochondrial release of caspases-2 and -9 during the apoptotic process. Journal of Experimental Medicine 189 : 381-393

Publications 1998

Mounier, C., Hackeng,T.M., Schaeffer, F., Faure, G., Bon, C. & Griffin, J.H. Inhibition of prothrombinase by human secretory phospholipase A2 involves binding to factor Xa Journal of Biological Chemistry 273 : 23764-23772

Tavares, G.A., Souchon, H., Guérin, D.M.A., Lascombe, M.-B., Alzari, P.M. Structural studies of the cellulosome, in Carbohydrases from Trichoderma reesei and other microorganisms, edited by M. Claeyssens, W. Nerinckx, K. Piens, pp. 174-181. Royal Society of Chemistry: Cambridge

Alzari, P.M. Immunoglobulin-type domains, in Encyclopedia of Immunology (second edition), edited by I.M.Roitt & P.J.Delves. Academic Press: London

Terenzi, H., Alzari, P.M., Zakin, M.M. Structural features involved in the formation of a complex between the monomeric or the dimeric form of Rev-erb beta DNA-binding domain and its DNA reactive sites. Biochemistry 37 : 11488-11495

Béguin, P., Alzari, P.M. The cellulosome of Clostridium thermocellum. Biochemical Society Transactions, 26 : 178-185

Demangel, C., Rouyre, S., Alzari, P.M., Nato, F., Longacre, S., Lafaye, P., Mazie, J.C. Phage-displayed mimotopes elicit monoclonal antibodies specific for a malaria vaccine candidate. Biological Chemistry 379 : 65-70

Publications 1997

Guillouard, I., Alzari, P.M., Saliou, B., Cole, S.T. The carboxy-terminal C2-like domain of the alpha-toxin from Clostridium perfringens mediates calcium-dependent membrane recognition. Molecular Microbiology 26 : 867-876

Babino, A., Pritsch, O., Oppezzo, P., Du Pasquier, R., Roseto, A., Osinaga, E., Alzari, P.M. Molecular cloning of a monoclonal anti-tumor antibody specific for the Tn antigen and expression of an active single-chain Fv fragment. Hybridoma 16 : 317-324

Tavares, G., Béguin, P., Alzari, P.M. The crystal structure of a type I cohesin domain at 1.7 Å resolution. Journal of Molecular Biology 273 : 701-713

Osinaga, E., Tello, D., Batthyani, C., Bianchet, M., Tavares, G., Durán, R., Cerveñansky, C., Camoin, L., Roseto, A., Alzari, P.M. Amino acid sequence and three-dimensional structure of the Tn-specific isolectin B4 from Vicia villosa. FEBS Letters 412 : 190-196

Eckenberg, R., Xu, D., Moreau, J.-L., Bossus, M., Mazié, J.-C., Tartar, A., Liu, X. Y., Alzari, P.M., Bertoglio, J., Thèze, J. Analysis of human IL-2/IL-2 receptor beta chain interactions: monoclonal antibody H2-8 and new IL-2 mutants define the critical role of alpha helix A of IL-2. Cytokine 9, 488-498

Lorenzo, H.K., Farber, D., Germain, V., Acuto, O., Alzari,P.M. The MBP-fusion protein restores the activity of the first phosphatase domain of CD45. FEBS Letters 411 : 231-235

Berthet, S., Nykyri, L.M., Bravo, J., Maté, M.J.,Berthet-Colominas, C., Alzari, P.M., Koller, F., Fita, I. Crystallization and preliminary structural analysis of catalase-A from Saccharomyces cerevisiae. Protein Science, 6, 481-483

Diaw,L., Magnac, C., Pritsch, O., Buckle, M., Alzari, P.M., Diguiero,G. Structural and affinity studies of IgM polyreactive natural antibodies. Journal of Immunology 158 : 968-976