Proteomics platform - Julia CHAMOT-ROOKE

Activities of the platform

Proteomic analyses are focused on microorganisms (bacteria, viruses, parasites, yeast) and their interactions with their host. Technologies available on the platform are two-dimensional electrophoresis, liquid chromatography, mass spectrometry and several tools dedicated to proteomic analysis. In recent years our equipment and methodologies have evolved to achieve analyses more accurate and more robust. The analyses carried out concern global proteomics, comparative or quantitative proteomics, identification of partners in protein complexes and identification of post translational modifications.

The three most significant publications :
1. Tafelmeyer P, Laurent C, Lenormand P, Rousselle JC, Marsollier L, Reysset G, Zhang R, Sickmann| A, Stinear TP, Namane A, Cole ST. (2008) Comprehensive Proteome Analysis of Mycobacterium ulcerans and Quantitative Comparison of Mycolactone Biosynthesis. Proteomics,8(15):3124-38

2. Morales MA, Watanabe R, Laurent C, Lenormand P, Rousselle JC, Namane A, Späth GF. (2008) Phosphoproteomic analysis of Leishmania donovani pro- and amastigote stages. Proteomics 8(2):350-63

3. Lebreton A, Rousselle JC, Lenormand P, Namane A, Jacquier A, Fromont-Racine M, Saveanu C. (2008) 60S ribosomal subunit assembly dynamics defined by semiquantitative mass spectrometry of purified complexes. Nucleic Acid Research, 36(15): 4988-99.