Publications 2005-2009

1. Vichier-Guerre, S.; Jestin, J. L. Characterisation of a DNA polymerase highly mutated along the template binding interface. Molecular Biotechnology sous presse.

2. Vidalain, P. O.; Tangy, F.; Jacob, Y.; Lucas-Hourani, M.; Munier-Lehmann, H.; Lupan, A. A mammalian cell-based screening assay to identify inhibitors of alphaviruses WO 2009/068998, 04/06/2009, 2009. 

3. Auvynet, C.; Topalis, D.; Caillat, C.; Munier-Lehmann, H.; Seclaman, E.; Balzarini, J.; Agrofoglio, L. A.; Kaminski, P. A.; Meyer, P.; Deville-Bonne, D.; El Amri, C. Phosphorylation of dGMP analogs by vaccinia virus TMP kinase and human GMP kinase. Biochem Biophys Res Commun 2009, 388, 6-11.

 4. Vidalain, P. O.; Tangy, F.; Munier-Lehmann, H.; Lupan, A.; Lucas-Hourani, M.; Jacob, Y. A cell-based screening assay to identify molecules that stimulate IFN-alpha/beta target genes. US 12/190.048 08/12/2008, 2008.

5. Topalis, D.; Alvarez, K.; Barral, K.; Munier-Lehmann, H.; Schneider, B.; Veron, M.; Guerreiro, C.; Mulard, L.; El-Amri, C.; Canard, B.; Deville-Bonne, D. Acyclic Phosphonate Nucleotides and Human Adenylate Kinases: Impact of a Borano Group on α-P Position. Nucleosides, Nucleotides and Nucleic Acids 2008, 27, 319 - 331.

6. Malliavin, T. E.; Munier-Lehmann, H.; Stoven, V. Virtual Screening of the Guanylate Monophosphate Kinase (GMPK) Family: Investigating the Rules of Ligand Specificity Letters in Drug Design & Discovery 2008, 5, 319-326.

7. Kaminski, P. A.; Dugué, L.; Pochet, S. Expanding substrate specificity of nucleoside 2’-deoxyribosyltransferase. Nucleic Acids Symp Ser (Oxf) 2008, 495-6.

8. Kaminski, P. A.; Dang, C. V. Methods for identifying anti-tumor and/or anti-angiogenesis drugs with Rcl deoxynucleoside 5’ -monophosphate N-glycosidase as the target 2008.

9. Kaminski, P. A.; Dacher, P.; Dugué, L.; Pochet, S. In vivo reshaping the catalytic site of nucleoside 2’-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution. J Biol Chem 2008, 283, 20053-9.

10. Gasse, C.; Douguet, D.; Huteau, V.; Marchal, G.; Munier-Lehmann, H.; Pochet, S. Substituted benzyl-pyrimidines targeting thymidine monophosphate kinase of Mycobacterium tuberculosis: Synthesis and in vitro anti-mycobacterial activity. Bioorg Med Chem 2008, 16, 6075-85.

11. Familiar, O.; Munier-Lehmann, H.; Negri, A.; Gago, F.; Douguet, D.; Rigouts, L.; Hernandez, A. I.; Camarasa, M. J.; Perez-Perez, M. J. Exploring acyclic nucleoside analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase. ChemMedChem 2008, 3, 1083-93.

12. Caillat, C.; Topalis, D.; Agrofoglio, L. A.; Pochet, S.; Balzarini, J.; Deville-Bonne, D.; Meyer, P. Crystal structure of poxvirus thymidylate kinase: An unexpected dimerization has implications for antiviral therapy. Proceedings of the National Academy of Sciences of the United States of America 2008, 105, 16900-16905.

13. Binesse, J.; Delsert, C.; Saulnier, D.; Champomier-Verges, M. C.; Zagorec, M.; Munier-Lehmann, H.; Mazel, D.; Le Roux, F. Metalloprotease vsm is the major determinant of toxicity for extracellular products of Vibrio splendidus. Appl Environ Microbiol 2008, 74, 7108-17.

14. Van Daele, I.; Munier-Lehmann, H.; Froeyen, M.; Balzarini, J.; Calenbergh, S. V. Rational Design of 5’-Thiourea-Substituted alpha-Thymidine Analogues as Thymidine Monophosphate Kinase Inhibitors Capable of Inhibiting Mycobacterial Growth. J Med Chem 2007, 50, 5281-5292.

15. Topalis, D.; Kumamoto, H.; Velasco, M. F. A.; Dugué, L.; Haouz, A.; Alexandre, J. A. C.; Gallois-Montbrun, S.; Alzari, P. M.; Pochet, S.; Agrofoglio, L. A.; Deville-Bonne, D. Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives - a screening based on affinity for the UMP-CMP binding site. Febs Journal 2007, 274, 3704-3714.

16. Topalis, D.; Kumamoto, H.; Alexandre, J.; Dugué, L.; Pochet, S.; Berteina-Raboin, S.; Agrofoglio, L.; Deville-Bonne, D. Looking for new pyrimidine acyclic nucleotide analogues designed for phosphorylation by human ump-cmp kinase. Nucleosides, Nucleotides & Nucleic Acids 2007, 26, 1369-1373.

17. Tischer, W.; Ihlenfeldt, H.-G.; Barzu, O.; Sakamoto, H.; Pistotnik, E.; Marliere, P.; Pochet, S. Enzymatic synthesis of deoxyribonucleosides. US 07229797, Jun 12 2007, 2007.

18. Poncet-Montange, G.; Assairi, L.; Arold, S.; Pochet, S.; Labesse, G. NAD Kinases Use Substrate-assisted Catalysis for Specific Recognition of NAD. J. Biol. Chem. 2007, 282, 33925-33934.

19. Ofiteru, A.; Bucurenci, N.; Alexov, E.; Bertrand, T.; Briozzo, P.; Munier-Lehmann, H.; Gilles, A. M. Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase. Febs J 2007, 274, 3363-733.

20. Moriou, C.; Thomas, M.; Adeline, M. T.; Martin, M. T.; Chiaroni, A.; Pochet, S.; Fourrey, J. L.; Favre, A.; Clivio, P. Crystal structure and photochemical behavior in solution of the 3’-N-Sulfamate analogue of thymidylyl(3’-5’)thymidine. Journal of Organic Chemistry 2007, 72, 43-50.

21. Ghiorghi, Y. K.; Zeller, K. I.; Dang, C. V.; Kaminski, P. A. The C-MYC target gene Rcl (C6orf108) encodes a novel enzyme : Deoxynucleoside 5’-monophosphate N-glycosidase. J Biol Chem 2007, 282, 8150-8156.

22. Gasse, C.; Huteau, V.; Douguet, D.; Munier-Lehmann, H.; Pochet, S. A New Family of Inhibitors of Mycobacterium Tuberculosis Thymidine Monophosphate Kinase. Nucleosides, Nucleotides and Nucleic Acids 2007, 26, 1057 - 1061.

23. Alexandre, J. A. C.; Roy, B.; Topalis, D.; Pochet, S.; Perigaud, C.; Deville-Bonne, D. Enantioselectivity of human AMP, dTMP and UMP-CMP kinases. Nucleic Acids Research 2007, 35, 4895-4904.

24. Vichier-Guerre, S.; Ferris, S.; Auberger, N.; Mahiddine, K.; Jestin, J. L. A population of thermostable reverse transcriptases evolved from Thermus aquaticus DNA polymerase I by phage display. Ang Chem Int Ed Engl 2006, 45, 6133-7.

25. Van Daele, I.; Munier-Lehmann, H.; Hendrickx, P. M.; Marchal, G.; Chavarot, P.; Froeyen, M.; Qing, L.; Martins, J. C.; Van Calenbergh, S. Synthesis and Biological Evaluation of Bicyclic Nucleosides as Inhibitors of M. tuberculosis Thymidylate Kinase. ChemMedChem 2006, 1, 1081-90.

26. Kamiya, H.; Cadena-Amaro, C.; Dugué, L.; Yakushiji, H.; Minakawa, N.; Matsuda, A.; Pochet, S.; Nakabeppu, Y.; Harashima, H. Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein. Journal of Biochemistry 2006, 140, 843-849.

27. Hible, G.; Christova, P.; Renault, L.; Seclaman, E.; Thompson, A.; Girard, E.; Munier-Lehmann, H.; Cherfils, J. Unique GMP-binding site in Mycobacterium tuberculosis guanosine monophosphate kinase. Proteins 2006, 62, 489-500.

28. Freire, T.; Bay, S.; Vichier-Guerre, S.; Lo-Man, R.; Leclerc, C. Carbohydrate antigens: synthesis aspects and immunological applications in cancer. Mini Rev Med Chem 2006, 6, 1357-1373.

29. Frachon, E.; Bondet, V.; Munier-Lehmann, H.; Bellalou, J. Multiple microfermentor battery: a versatile tool for use with automated parallel cultures of microorganisms producing recombinant proteins and for optimization of cultivation protocols. Appl Environ Microbiol 2006, 72, 5225-31.

30. Bellinzoni, M.; Haouz, A.; Grana, M.; Munier-Lehmann, H.; Shepard, W.; Alzari, P. M. The crystal structure of Mycobacterium tuberculosis adenylate kinase in complex with two molecules of ADP and Mg2+ supports an associative mechanism for phosphoryl transfer. Protein Sci 2006, 15, 1489-93.

31. Volmer, R.; Bajramovic, J. J.; Schneider, U.; Ufano, S.; Pochet, S.; Gonzalez-Dunia, D. Mechanism of the antiviral action of 1-beta-D-arabinofuranosylcytosine on Borna disease virus. Journal of Virology 2005, 79, 4514-4518.

32. Van Daele, I.; Munier-Lehmann, H.; Van Calenbergh, S. Exploring high affinity inhibitors of M. tuberculosis thymidylate kinase. In M. Hocek, E., Ed. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague: 2005; Vol. 7, pp 313-314.

33. Topalis, D.; Collinet, B.; Gasse, C.; Dugué, L.; Balzarini, J.; Pochet, S.; Deville-Bonne, D. Substrate specificity of vaccinia virus thymidylate kinase. Febs Journal 2005, 272, 6254-6265.

34. Jestin, J. L.; Vichier-Guerre, S. How to broaden enzyme substrate specificity: Strategies, implications and applications. Research In Microbiology 2005, 156, 961-966.

35. Fioravanti, E.; Adam, V.; Munier-Lehmann, H.; Bourgeois, D. The crystal structure of Mycobacterium tuberculosis thymidylate kinase in complex with 3’-azido-deoxythymidine-monophosphate (AZTMP) suggests a mechanism for competitive inhibition. Biochemistry 2005, 44, 130-7.

36. Douguet, D.; Munier-Lehmann, H.; Labesse, G.; Pochet, S. LEA3D : a computer-aided ligand design for structure-based drug design. J. Med. Chem. 2005, 48, 2457-68.

37. Deville-Bonne, D.; Dugué, L.; Gallois-Montbrun, S.; Veron, M.; Pochet, S. Human UMP-CMP kinase specificity for natural and antiviral analogs using competition fluorescence experiments. Antiviral Research 2005, 65, A76-A76. 38. Cadena-Amaro, C.; Pochet, S. Efficient incorporation of 1-(2-deoxy-beta-D-ribofuranosyl)-2-oxo-imidazole-4-carboxamide into DNA via a suitable convertible phosphoramidite derivative. Tetrahedron 2005, 61, 5081-5087.

39. Cadena-Amaro, C.; Delepierre, M.; Pochet, S. Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine. Bioorganic & Medicinal Chemistry Letters 2005, 15, 1069-1073.