Surface Plasmon Resonance

Contact: Sylviane HOOS

SPR microfluidic biosensors allow the label-free analysis of molecular interactions in real time. For that purpose, one of the interaction partners is immobilized, covalently or not, on a sensorchip, and the other one is brought in contact with the chip using a continuous buffer flow.
This technique is especially suited for the determination of association (kon) and dissociation (koff) rates between biologicals macromolecules.
The PFBMI has three SPR biosensors, allowing to go from training and experimental set-up to medium throughput structure-function studies.

The Biacore X100 (GE Healthcare) is principally designed to drive new users toward the conception and realization of interactions experiments, by the way of user friendly wizards for assay design and software support for the analysis of interaction sensorgrams.

The Biacore 2000 (GE Healthcare) allows to following the interactions of a molecule with 4 independent immobilized partners. Series of experiments with a high degree of reproducibility can be performed using a totally automatable software.

The ProteOn XPR36 (Biorad) uses a specific core fluidic system to enable the simultaneous analysis of the interaction between up to six ligands with up to six analytes in a 36 spots array. With itsa flexible and versatile software for experiment design, the ProteOn allows the completion of more experiments in less time, opening the way toward medium-throughput studies involving several hundred of compounds.