Research / Scientific departments / Structural Biology and Chemistry / Units and Groups / Biophysics of Macromolecules and their interactions / Technologies / Analytical Ultracentrifugation
Analytical Ultracentrifugation
Contact: Bertrand RAYNAL
PFBMI have two ultracentrifuges from Beckman-Coulter, an Optima XL-A and a ProteomeLab XL-I. The latter one is equipped with a double detection system, UV/visible and interference.
The hydrodynamic behaviour of a macromolecule and its sedimentation coefficient are linked to its volume, its shape, its density and its mass.
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Analytical ultracentrifugation allows a direct measurment of the sedimentation ceofficient orthe mass of a macromolecule in solution. When an oligomer or of a non covalent complex are considered, the number of chains associated within the native molecule or the stœchiometry of the complex can thus be determined.
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Informations can also be obtained on the conformation of the macromolecules, the equilibrium constant of the assemblies and on the homogeneity of samples. |