Biophysics of Macromolecules and their interactions

Biophysique des macromolécules et de leurs interactions

Secondary and tertiary structure of macromolecules 
Relevant technologies at the PFBMI

Circular dichroism (CD)

Measurement of secondary and tertiary structures.
Abalyse of conformational changes
Kinetic of folding
Thermal and chemical stability of folding


Fluorescence spectroscopy

Analysis of tertiary conformation, of thermal and chemical stability and of molecular interactions: 

intensity, anisotropy and fluorescence life-time.


Infrared spectroscopy (FTIR) 

Secondary structures of macromolecules, specially proteins with high content in  ß sheath
Spectroscopie infrarouge FTIR300
The PFBMI can advise you or direct you on your choices and give you information on:

* Experiments requirements and constraints
* Lenght if experiments
* Estimations and calculations of physico-chemical parameters
* Methodological links and softwares to be used