Biophysics of Macromolecules and their interactions

Biophysique des macromolécules et de leurs interactions

Kinetic and thermodynamic properties of interactions
Relevant technologies at the PFBMI

Surface Plasmon Resonance (SPR)  

Analyse of molecular interaction in real time : 
association (kon) and dissociation (koff) kinetic constants
Biacore X100

Fluorescence spectroscopy

Analysis of tertiary conformation, of thermal and chemical stability and of molecular interactions: 

intensity, anisotropy and fluorescence life-time.


Fluorescence  « stopped-flow  » 

Kinetic of interactions and enzyme activity

Fluo2 stopped-flow260
The PFBMI can advise you or direct you on your choices and give you information on:

* Experiments requirements and constraints
* Lenght if experiments
* Estimations and calculations of physico-chemical parameters
* Methodological links and softwares to be used