Biophysique des macromolécules et de leurs interactions

Size and shape of macromolecules
Relevant technologies at the PFBMI

Dynamic light scattering (DLS) 

Quantification of diffusion rate and, by deduction of hydrodynamic radius (a value that depends on their mass and shape, notably)

Diffusion dynamique de la lumière DLS300

Static light scattering and viscometry 

Measure of mass and intrinsic viscometry of macromolecules

Oligomerisation state and stœchiometry of assemblies

photo Viscotek GPCmax-TDA550

Analytical Ultracentrifugation

Measurement of mass and shape of macromolecules in solution.

Oligomerisation state, stœchiometry, equilibrium constant of assemblies

The PFBMI can advise you or direct you on your choices and give you information on:

* Experiments requirements and constraints
* Lenght if experiments
* Estimations and calculations of physico-chemical parameters
* Methodological links and softwares to be used