Anaerobic Bacteria and Toxins Unit
Clostridial binary toxins (C. perfringens iota, toxin C. botulinum C2 toxin) represent attractive tools to vehicle heterogeneous proteins into target cells, since the functional domains are located in two separate proteins. We have found that the binding component (Ib) of iota toxin form channels into lipid bilayer and that Ib when bound to target cell membrane, oligomerizes and is then distributed in detergent-resistant membrane microdomains permitting the translocation of the enzymatic component (Ia) through the endosomal membrane.
Large clostridial toxins such as LT from C. sordellii glucosylates various
Expert work on botulism includes: preparation and production of recombinant antigens of BoNTs, production of specific polyclonal and monoclonal antibodies, genotyping and sequencing of BoNT gene of C. botulinum strains.
The CNR of anaerobic bacteria and botulism is involved in identification of anaerobe strains isolated by hospital laboratories, as well as in diagnostic and survey of botulism in France . Taxonomic studies of certain groups of anaerobes, as well as genotyping and characterization of C. difficile emergent strains are in progress