Research / Scientific departments / Developmental Biology & Stem Cells / Units and groups / Mouse genetics engineering center (CIGM)
Mouse Genetics Engineering Center (CIGM)
| Francina Langa Vives francina@pasteur.fr |
CIGM:
Officially created in June 2003, the Centre d’Ingénierie Génétique Murine (CIGM) is a novel technological facility at Institut Pasteur. The CIGM is open to the Pasteur scientific community (and also to external customers) interested in new transgenic, knock-out or knock-in murine models.
The CIGM aims to generate genetically-engineered mice either by:
The CIGM aims to generate genetically-engineered mice either by:
Classical transgenesis techniques, consisting of microinjection of the gene of interest (ADN fragment, BAC or YAC) into the pronuclei of fertilized eggs.
The service is available in hybrid (B6SJLF1, B6CBAF1) and pure (C57BL6/N, FVB/N) genetic backgrounds.
Targeted mutation by homologous recombination in ES cells and germline transmission.
Chimeras crossing for the obtention of heterozygous mice for the desired mutation.
The CIGM also provides advice for optimal design of both types of transgenic DNA constructions.