Dynamics of Host-Pathogen Interactions

Unit : Dynamics of Host-Pathogen Interactions

Unit leader: Jost Enninga, Ph.D.

Our unit “Dynamique des interactions hôte-pathogène” or “Dynamics of host pathogen-interactions” was created in January 2008. We are developing novel approaches to investigate the interactions between pathogens and their hosts in single cells in real time.

In particular, we are interested in deciphering the molecular and cellular basis  of how a number of bacterial pathogens, such as Shigella or Salmonella enter host cells during the infection process. To achieve this, we film these invasion events using innovative fluorescence microscopy. More specifically, we focus on the establishment of novel assays that allow us to monitor pathogen invasion; simultaneously tracking a large number of parameters involved in the invasion processes. In doing this we are able to correlate the pathogen behavior, its intracellular localization and the host responses it induces in single cells in real time.

We are convinced that deciphering the molecular and cellular details of the invasion strategies used by the bacterial pathogens will identify novel drug targets and will provide new insight into fundamental cell biological processes.

We have obtained generous funding for our research by the following agencies and institutions during recent years and for the coming years: the Institut Pasteur, the Agence Nationale pour la Recherche (ANR), the European Union (ERC program and Marie Curie program), the Fondation Schlumberger pour l'Education et la Recherche, the Fundação para a Ciência e Tecnologia (FCT), the French Foreign Ministry, EMBO and HFSPO. We would like to thank them for their support.

Our unit

Jost ENNINGA                           - Principal investigator
Allon WEINER                            - Postdoc
Juliane LIPPMANN                     - Postdoc
Jennifer FREDLUND                  - Postdoc
Nora MELLOUK                          - Postdoc
José VIEIRA dos SANTOS         - Ph D student
Camille REY                               - Ph D student
Virginie STEVENIN                     - Ph D student
Patricia LATOUR-LAMBERT      - Engineer
Laurence LANGLAIS                  - Assistant
Laurent AUDRY                          - Technician, webmaster

Selected recent publications

1. Mellouk N, Weiner A, Aulner N, Schmitt C, Elabaum M, Shorte SL, Danckaert A, Enninga J, Shigella subverts the host recycling compartmeent to rupture its vacuaole. Cell Host Microbe 2014 Oct 16(4)517-530

2. Keller C, Mellouk N, Danckaert A, Simeone R, Brosch R, Enninga J, Bobard A., Single cell measurements of vacuolar rupture caused by intracellular pathogens . J Vis Exp. 2013 Jun 12;(76):e50116. doi: 10.3791/50116.

3.Simeone R. (*), Bobard A. (*), Lippmann J., Bitter W., Majlessi L., Brosch R. (*) and Enninga J. (*), Phagosomal translocation of Mycobacterium tuberculosis results in toxicity and host cell death, PLoS Pathogens, 2012 Feb;8(2):e1002507.

If you like to obtain more information on what we are doing, follow the link :

If you like to contact us,

Email:         laurence.langlais@pasteur.fr

Our address is :

Groupe “Dynamique des interactions hôte-pathogène”
Institut Pasteur
25, rue du Dr. Roux
75724 Paris