Equipment
2D Electrophoresis
- Protean IEF Cell (BioRad) for the first dimension
- HPE FlatTop Tower (Serva Electrophoresis) for the second dimension
- Typhoon 9400 (GE HealthCare) for fluorescent gels.
Excision Digestion
- Screen Picker (Proteomics Consult). Spot cutting on fluorescent gels (DIGE, LavaPurple, etc)
- Digestion and Spotting Protein digestion and sample spotting before mass spectrometry analysis.
Mass Spectrometers
- LTQ Orbitrap Velos with a ETD module (Thermo Fisher) equipped with a nano-HPLC Ultimate 3000 (Dionex)
- 4800 MALDI TOF/TOF (AB-MDS-SCIEX) with a nano-HPLC Ultimate 3000 (Dionex) and a spotting robot.
- Qstar XL (AB-MDS-SCIEX) equipped with a nano-HPLC Ultimate (Dionex)