Jean-Claude MANUGUERRA
Laboratory for urgent response to biological threats [CIBU]
Institut Pasteur 25-28 rue du docteur Roux, 75015 Paris
 
Research area of the Unit
 
The Laboratory for urgent response to biological threats (CIBU) was created in 2002, under the impulse of the Ministry of Health and Institut Pasteur (IP) to respond to "specialised biological urgencies". These urgencies are dealt with in the event of epidemics, accidents or bioterrorist attacks using biological weapons which can endanger Public Health. CIBU being in a research institute, its core missions in Public Health are rooted in research activities, which are focused on two main areas: 1/ Theme 1: Detection and identification of pathogens using massively parallel technologies and 2/ Theme 2: Survival of viruses outside their hosts. The choice of research subjects is directly linked to our Public Health involvement.
Since its creation, CIBU has been directly involved a number of major health crises, all of them from natural events
 
Contribution to the programme
-          In field outbreak investigation
-          In site sample preparation and transport
-          Infectious risk management and sample handling
-          Wide spectrum screening for major known pathogens (virus/bacteria culture, detection by molecular biology tools, serology)
-          Serosurveys
 
References over the past 5 years
 
1.         Berthet N, Dickinson P, Filliol I, Reinhardt AK, Batejat C, Vallaeys T, et al. Massively parallel pathogen identification using high-density microarrays. Microb Biotechnol. 2008;1(1):79-86.
2.         Berthet N, Leclercq I, Dublineau A, Shigematsu S, Burguiere AM, Filippone C, Gessain A, Manuguerra J.C. High-density resequencing DNA microarrays in public health emergencies. Nat Biotechnol. 2010 Jan;28(1):25-7.
3.         Berthet N, Nakouné EW, E., Selekon BB, A.M. Manuguerra, J.C., Gessain A, Mirdad K. Maculopapular lesions in two young Pygmies in the Central African Republic. The Lancet. 2011;378(9799):1354.
4.         Berthet N, Reinhardt AK, Leclercq I, van Ooyen S, Batejat C, Dickinson P, et al. Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR. BMC Mol Biol. 2008;9:77.
5.         Dublineau A, Batejat C, Pinon A, Burguiere AM, Leclercq I, Manuguerra JC. Persistence of the 2009 pandemic influenza A (H1N1) virus in water and on non-porous surface. PLoS One. 2011;6(11):e28043.
6.         Gouilh MA, Puechmaille SJ, Gonzalez JP, Teeling E, Kittayapong P, Manuguerra JC. SARS-Coronavirus ancestor's foot-prints in South-East Asian bat colonies and the refuge theory. Infect Genet Evol. 2011 Jul 8.
7.         Leclercq I, Berthet N, Batejat C, Rousseaux C, Dickinson P, Old IG, et al. Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm. BMC Genomics. 2010;11:586.