Dynamics of host-pathogen interactions Unit
Institut Pasteur 25-28 rue du docteur Roux, 75015 Paris
Research area of the Unit
The research group of Dr. ENNINGA « Dynamics of host-pathogen interactions » establishes novel tools that yield information on the molecular details of bacterial pathogen infection in the context of single living cells with unprecedented spatiotemporal resolution. A focus is put on the following aspects:
1)      the pathogen behavior during host cell entry.
2)      intracellular localization of the invading pathogens
3)      induced host responses within the host cells.
To do these correlative studies, Dr. ENNINGA has implemented a novel methodology that allows the tracking of vacuolar escape of invasive pathogens in living cells in real time. Vacuolar escape is a major step of the invasion process of a number of pathogens, including Listeria, Shigella, and Rickettsia; their change of intracellular localization from the vacuolar to the cytoplasmic compartment requires an adaptation of the host strategy to clear these intracellular pathogens, and the pathogens have developed means to counterbalance these effects.
Contribution to the programme
For the IBEID research program the group of Dr. ENNINGA will provide its tools to study the dynamics of pathogen entry into host cells at the single cell level. The technical tools that will be provided allow the investigation of viruses, bacteria, or parasites during host cell invasion with unprecdented spatiotemporal resolution. The equipment for the study has been put in place in Dr. ENNINGA’s research group, and will be supplemented by the purchase of additional cutting edge microscopes. Furthermore, the group of Dr. ENNINGA has experience in studies that involve high-content/high-throughput approaches. Together with other participating research groups of this research program, for example Dr. Arenzana, Dr. Olivo-Marin, and Dr. Shorte, an infrastructure will be established with the aim to provide an optimal surrounding for studies that involve high-content/high-throughput technologies on the genome-wide level.
References over the past 5 years
  1. Ehsani S, Santos JC, Rodrigues CD, Henriques R, Audry L, Zimmer C, Sansonetti P, Tran Van Nhieu G, Enninga J. Hierarchies of host factor dynamics at the entry site of Shigella flexneri during host cell invasion. Infect Immun. 2012 Jul;80(7):2548-57.
  2. Simeone R., Bobard A., Lippmann J., Bitter W., Majlessi L., Brosch R. and Enninga J., Phagosomal translocation of Mycobacterium tuberculosis results in toxicity and host cell death, PLoS Pathogens, 2012 Feb;8(2):e1002507.
  3. Cebrian I, Visentin G., Blanchard N., Bobard A., Moita C., Enninga J., Moita LF, Amigorena S., Savina A. Sec22b controls the recruitment of endoplasmic reticulum to phagosomes in dendritic cells Cell, 2011 Dec 9;147(6):1355-68.
  4. Ray K., Bobard A., Danckaert A., Clair C., Ehsani S., Tang C., Sansonetti P., Tran Van Nhieu G., Enninga J. Tracking the dynamic interplay between bacterial and host factors during pathogen-induced vacuolar rupture in real time by fluorescence microscopy. Cellular Microbiology 2010. Apr 1;12(4):545-56.
  5. Paz I., Sachse M., Dupont N., Cederfur C., Enninga J., Leffler H., Poirier F., Prevost MC., Lafont F., Sansonetti P. Galectin-3, a potential novel marker for vacuole lysis. Cellular Microbiology 2010. Apr 1;12(4):530-44.
  6. Simpson N., Audry L., and Enninga J. Tracking the secretion of fluorescently-labeled type III effectors from single bacteria in real time. Methods in Mol Biology, 2010. 2010;619:241-56.
  7. Ehsani S., Rodrigues D.C., Enninga J. Turning on the spotlight - Using light to monitor and characterize bacterial effector secretion and translocation. 2009. Curr Op in Microbiol. 2009 Feb;12(1):24-30.