IV - III - The Pasteur Culture Collection of Cyanobacteria (PCC)
It was fortunate that, with his move to the Institut Pasteur from Berkeley in 1971, Roger Y. Stanier brought with him the Berkeley Culture Collection, and encouraged his small team (Josette Deruelles, Rosmarie Rippka and John B. Waterbury) to isolate, purify and characterize many more strains of this group of photosynthetic prokaryotes, for which he (Stanier, 1974) proposed the name "cyanobacteria". A detailed description of about 170 cyanobacterial strains in axenic cultures was given by Rippka et al. (1979).
Since that time the number of axenic strains in the PCC has increased to more than 750, and now represents a culture collection of a large number of genera and species isolated from a wide variety of habitats. This achievement was only possible due to the skill and patience of many colleagues in the Unité de Physiologie Microbienne, since 2001 called Unité des Cyanobactéries, at the Institut Pasteur. In addition, many strains have been supplied, either as impure or axenic isolates, by investigators from other laboratories in all parts of the world. It is not possible in this introduction to thank all of these collaborators individually. However, their names are cited, and their contributions accredited under the heading "strain histories".
This temporary PCC database of the CRBIP website, summarizes the history of all cyanobacterial isolates deposited in the PCC prior to 1993 and takes into account some recent nomenclatural changes with respect to the PCC catalogue published in 1992 (Rippka & Herdman, 1992). More recent accessions not mentioned in this website but cited in the literature, are available upon special request.
The Pasteur Culture Collection of Cyanobacteria (PCC) is supported by the Institut Pasteur, which has provided the space and facilities for the PCC since 1971, and by the Centre National de la Recherche Scientifique (URA 2172).
The axenic strains of the PCC are maintained in the Unité des Cyanobactéries. The PCC is one of the specialized Culture Collections of the Institut Pasteur.
The PCC is a member of the World Federation for Culture Collections and is registered with the World Data Centre for Microorganisms under number WDCM481.
In compliance with the instructions of the ECCO (European Culture Collections’ Organization) the PCC ensures the following services:
• the maintenance of axenic cyanobacterial strains in accordance with established criteria of quality;
• supply of cyanobacterial strains without restrictions of access other than those imposed by legal and ethical requirements;
• continuous enlargement of the Culture Collection by addition of new strains;
• publication of a catalogue listing the available strains;
• information concerning the cultures ;
The PCC is aided in these tasks by the CNCM, which assures the administration, packing and dispatch of the strains.
Staff members of the PCC
(Research scientistand and Curator)
Bénédicte Bénédic (Secretary)
Thierry Laurent - Thérèse Coursin (technical staff)
Staff members of the CNCM
For information on the taxonomy of cyanobacteria, the CRBIP website is invited to consult the second edition of Bergey’s Manual of Systematic Bacteriology, Volume One: The Archaea and the Deeply Branching and Phototrophic Bacteria (2001). D.R. Boone, R.W. Castenholz and G.M. Garrity (Eds.). Springer, New-York.
Description and properties of cyanobacterial strains
The information given in the description of each strain is presented in the following order.
The strain history
Habitat, location and year of sampling
Coidentity and Culture Collections
Although some of the culture collections indicate the state of purity of their strains, this property being subject to change, this information is not provided on the CRBIP website.
Properties of the strains
Information concerning the strain properties (such as cell size, chromatic adaptation, toxin production, motility, salt tolerance, heterotrophic potential, nitrogen fixation, cyanophage sensitivity, mean DNA base composition and genome size) have not yet been entered in the PCC database of the CRBIP website. If they were known or applicable prior to 1991, they are summarized in the first catalogue of the PCC (Rippka & Herdman, 1992), in which data from the following major references, grouped by subject, were compiled.
Cell size, structural and developmental features: Stanier et al. (1971); Rippka et al. (1974, 1979); Waterbury & Stanier (1977; 1978); Rippka & Cohen-Bazire (1983); Guglielmi et al. (1984).
Pigments and complementary chromatic adaptation: Tandeau de Marsac (1977); Bryant (1982).
Mean DNA base composition and genome size: Stanier et al. (1971); Herdman et al. (1979a, 1979b); Stam (1980); Lachance (1981); Wilmotte & Stam (1984).
Salt requirements or tolerance: Stanier et al. (1971); Rippka et al. 1979; Waterbury & Stanier (1977; 1978); Reed et al. (1984); Mackay et al. (1984).
Host to cyanophages: Safferman & Morris, 1963; 1964; Rippka & Cohen-Bazire (1983) and references therein.
Type strains: Only Prochlorothrix hollandica strain PCC 9006T (Burger-Wiersma et al.,1986; 1989) and Prochlorococcus marinus subsp. pastoris strain PCC 9511T (Rippka et al., 2000) have validly been published under the Bacteriological Code of Nomenclature.
References strains: Strains previously (Rippka et al., 1979) designated as reference strains of genera are indicated at the end of the respective strain description. If subsequently another reference strain was chosen or nomenclatural changes proposed (Waterbury & Rippka, 1989; Rippka & Herdman, 1992; Herdman et al., 2001; Rippka et al., 2001a; Rippka et al., 2001b, Herdman et al. 2005), the more current assignments are also mentioned.
The following symbols are used in the catalogue data sheet
|Habitat, location and year of isolation|
|Corresponding numbers in other collections|
|Properties of the strain|
|Particular features of the strain|
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