Sixth International Symposium on

Positive Strand RNA Viruses

May 28-June 2, 2001

INSTITUT PASTEUR, Paris, France

M. Brahic, Institut Pasteur, Paris, France M. Brinton, Georgia State University, Atlanta, GA, USA V. Deubel, Centre de Recherche Mérieux-Pasteur, Lyon, France A.L. Haenni, Institut Jacques Monod, Paris, France H. Laude, Institut National de la recherche Agronomique, Jouy en Josas, France

P. Ahlquist, University of Wisconsin, Madison, WI, USA A. Borman, Institut Pasteur, Paris, France J. Dubuisson, Institut de Biologie, Lille, France F.A. Ennis, University of Massachusetts, Worcester, MA, USA D.E. Griffin, Johns Hopkins University School of Public Health, Baltimore, MD, USA F.X. Heinz, University of Vienna, Vienna, Austria K. Richards, Institut de Biologie Moléculaire des Plantes, Strasbourg, France W. Spaan, Leiden University Medical School, Leiden, The Netherlands

Monday May 28, 2001 10h00 – 20h00 Registration 13h30 – 17h00 OPEN SESSION - DENGUE AND JE VACCINES RESEARCH (WHO) 13h15 WHO research programme on dengue prevention and control Yuri PERVIKOV - Marie Paule KIENY 13h30 Current status in development and evaluation of live attenuated dengue vaccine (Mahidol University-Aventis Pasteur project) Jean-François SALUZZO 14h00 Dengue Vaccine Development in the US Department of Defense David VAUGHN 14h30 Development of chimeric dengue and JE vaccines Farshad GUIRAKHOO 15h00 Coffee break 15h30 Immunopathology of DHF and concerns for vaccine development Françis A. ENNIS 16h00 Design of field efficacy trials of dengue vaccines to prevent DHF M. MAMMEN 16h30 Development and trials of JE vaccines in Japan, China and USA Ichiro KURANE 17h00 End of the session 18h00 KEYNOTE PRESENTATION, POSITIVE STRAND RNA VIRUSES SYMPOSIUM IMMUNE MEMORY: REMEMBERING OUR VIRUSES (Rafi AHMED, Emory University, Atlanta, USA) 19h00 Welcome cocktail Tuesday May 29, 2001 Morning 8h45 FEMS Presentation – Richard BRAUN PLENARY SESSION I - VIRUS-HOST INTERACTIONS Chairpersons : Françis A. ENNIS, Michel BRAHIC 9h00 Dynamics of immune effector mechanisms controlling neurotropic MHV infection Cornelia BERGMANN, Keck School of Medecine, Los Angeles, USA 9h30 Cellular immune responses to dengue viruses : T Lymphocyte activation in DHF Ichiro KURANE, National Institute of Infectious Diseases, Tokyo, Japan 10h00 Switching the species tropism : an effective way to manipulate the feline corona virus genome Peter J.M. ROTTIER, Veterinary Faculty, Utrech, The Netherlands 10h30 Coffee Break 11h00 Post-transcriptional gene silencing has key role in the symptom attenuation and evolution of defective interfering RNAs in tombusvirus infected plants Jozsef BURGYAN, Agricultural Biotechnology Center, Gödöllo, Hungary 11h30 The role of PTB and brain PTB in translation regulation and picornavirus disease pathogenesis Evgeny PILIPENKO, University of Chicago, Chicago, USA 11h45 The leader protein of Theiler's virus inhibits immediate-early type-1 interferon production Thomas MICHIELS, University of Louvain, Brussels , Belgium 12h15 The search for the flavivirus resistance (FLV) gene in mice Andrey PERELYGIN, Georgia State University, Atlanta, USA 12h30 Lunch Afternoon 14h00 POSTER SESSION I PLENARY SESSION II -VIRUS ENTRY AND ASSEMBLY (1) Chairpersons : Franz X. HEINZ, Willy J.M. SPAAN 16h00 Crystal structure of the fusion glycoprotein E1 of Semliki forest virus: implications for the assembly of the virion Félix REY, CNRS, Gif sur Yvette, France 16h30 Structure of Dengue virus Richard KUHN, Perdue University, West Lafayette, USA 17h00 Norwalk virus binds to H type 1 histo-blood group antigen present on gastro-duodenal epithelial cells of "secretor" phenotype individuals Jacques LE PENDU, Institut de Biologie, Nantes, France 17h15 Coffee break 17h45 Pleomorphic and symmetrical organization of viral capsids in virus assembly and entry Holland R. CHENG, Karolinska Institute, Huddinge, Sweden 18h15 Receptor specificity determinants for the major and minor groups of human rhinovirus Jordi BELLA, School of Biological Sciences, Manchester, UK 18h45 Snapshots of the poliovirus cell entry pathway James HOGLE, Harvard Medical School, Boston, USA 19h15 End of the session Wednesday May 30, 2001 Morning PLENARY SESSION II -VIRUS ENTRY AND ASSEMBLY (2) Chairpersons : Ken RICHARDS, Edwin WESTAWAY 9h00 Alphavirus membrane fusion Margaret KIELIAN, Albert Einstein College of Medicine, New-York, USA 9h30 The machinery for flavivirus fusion with host cell membranes Franz X. HEINZ, University of Vienna, Vienna, Austria 10h00 Pivotal role of the transmembrane domains of hepatitis C virus envelope proteins in the biogenesis of a functional heterodimer Jean DUBUISSON, Institut de Biologie de Lille, Lille, France 10h30 Coffee break 11h00 Cooperation of an RNA packaging signal and a viral envelope protein in coronavirus RNA packaging Shinji MAKINO, Univesity of Texas, Galveston, USA 11h15 Coupling between replication and packaging of flavivirus RNA Alexander KHROMYKH, Sir Albert Sarzewski Virus Research Centre, Brisbane, Australia 11h45 3D structural determinants that control the cleavage of the prM translocation signal in dengue virus François PENIN, IBCP-CNRS, Lyon, France 12h15 Lunch Afternoon SHORT TALKS SESSION Chairpersons : Yi-Ling LIN, Katy KEAN 14h00 A reverse genetic system for coronaviruses Volker THIEL, University of Wuerzburg, Wuerzburg, Germany 14h15 Biological consequences of TGEV gene duplication and rearrangement Ralf BARIC, University of North Carolina, Chapel Hill, USA 14h30 Potyviral infection and its relationship with the translation initiation factor EIF4E Simon LEONARD, Institut Armand Frappier, Quebec, Canada 14h45 Interactions between piconaviruses and the cytoskeleton : functions of 2A proteinase Ernst KUECHLER, Institute of Medical Biochemistry, Vienna, Austria 15h00 The intracellular expression of the dengue M ectodomain leads to the rapid induction of apoptosis Philippe DESPRES, Institut Pasteur, Paris, France 15h15 Analysis of Bax-induced cell death and N-mediated hypersensitive response to TMV Christophe LACOMME, Scottish Crop Research Institute, Dundee, Scotland 15h30 Coffee break 16h00 The TMEVD5 locus controls the appearance of clinical signs in mice persistently infected with Theiler's virus Jean-François BUREAU, Institut Pasteur, Paris, France 16h15 Improvement of a plant virus based vector through DNA shuffling Rachel L. TOTH, Scottish Crop Research Institute, Dundee, Scotland 16h30 Vaccination against RSV infection using replicon-based vaccines Margaret CHEN, Swedish Institute for Infectious Disease Control, Solna, Sweden 16h45 - 18h45 POSTER SESSION I Evening : Gala Dinner. Guinguette Thursday May 31, 2001 Morning PLENARY SESSION III - GENE EXPRESSION AND REPLICATION (1) Chairpersons : Vadim AGOL, Anne Lise HAENNI 9h00 Cre-dependent uridylylation of Entero- and Rhinovirus VPgs : a mechanism designed for genome-specific initiation of RNA replication Eckard WIMMER, State University of New York Stony Brook, New-York, USA 9h30 Isolation of enzymatically-active replication complexes from feline calcivirus-infected cells Kim Y. GREEN, National Institutes of Health, Bethesda, USA 10h00 Cis and trans-acting factors in pestivirus RNA replication Sven-Erik BEHRENS, Institut für Virologie, Giessen, Allemagne 10h30 Coffee break 11h00 Corona- and arterivirus superfamily 1 helicases have 5'- to -3' duplex-unwinding activities that depend on the associated zinc finger structure John ZIEBUHR, University of Wurzburg, Wurzburg, Germany 11h15 A co-axial stack-like RNA-RNA interaction regulates transcription of a bipartite plant virus Steven LOMMEL, North Carolina State Univ., Raleigh, USA 11h45 Bromovirus and nodavirus RNA replication Paul AHLQUIST, University of Wisconsin, Madison, USA 12h15 Lunch Afternoon 14h00 POSTER SESSION II PLENARY SESSION III - GENE EXPRESSION AND REPLICATION (2) Chairpersons : Andrew BORMAN, Hubert LAUDE 16h00 Engineering coronavirus genomes as biosafe vectors Luis ENJUANES, CNB, CSIC, Madrid, Spain 16h30 Crucifer-infecting tobamovirus RNA contains an internal ribosome entry site elements Joseph ATABEKOV, Dept. of Virology, Moscow State University, Moscow, Russia 17h00 Mutation of the NS2Aa site of yellow fever virus reveals a role of NS2A in production of infectious particles Beate KUEMMERER, The Rockefeller University, New-York, USA 17h15 Coffee break 17h45 Luteovirus translation W. Allen MILLER, Plant Pathology Dept., Iowa State University, Ames, USA 18h15 Translational control by delayed RNA folding; identification of the kinetic trap Jan VAN DUIN, Leiden Institute of Chemistry, Leiden, The Nederlands 18h45 Nidovirus RNA synthesis dissected: nucleotides, membranes, amino acids, and a bit of zinc Eric J. SNIJDER, Dep of Virology, Leiden University Medical Center, Leiden, The Nederlands 19h15 End of the session Friday June 1st, 2001 Morning PLENARY SESSION IV - VIRUS EVOLUTION AND GENOMICS Chairpersons : Jean-Yves SGRO, Radu CRAINIC 9h00 Evolution of foot-and-mouth disease virus Esteban DOMINGO, Centro de Biologia Molecular « Severo Ochoa », Madrid, Spain 9h30 Adaptation to new hosts and Venezuelan equine encephalitis virus emergence Scott C. WEAVER, University of Texas Medical Branch, Galveston, USA 10h00 Ancient proteins of remarkable plasticity secure prosperity of +RNA viruses Alexander GORBALENYA, SAIC/NCI-Frederick, Frederick, USA 10h30 Coffee break 11h00 A viable chimera of plant and animal viruses Valerian DOLJA, Oregon State University, Corvallis, USA 11h30 A cellular J-domain protein modulates polyprotein processing and cytopathogenicity of an animal virus Norbert TAUTZ, Institut für Virologie, Giessen,Germany 12h00 A replicon based cell culture system for the hepatitis C virus Volker LOHMANN, Institut für Virologie, Mainz, Germany 12h30 Lunch Afternoon 14h00 POSTER SESSION II PLENARY SESSION V - STRATEGIES TO CONTROL VIRAL INFECTIONS (Sponsored by Eli Lilly and Company) Chairpersons : Vincent DEUBEL, Carol BLAIR 16h00 Cowpea mosaic virus as a versatile system for the expression of foreign peptides and proteins in legumes George P. LOMONOSSOFF, John Innes Centre, Norwich, UK 16h30 Novel inhibitors of hepatitis C RNA-dependent RNA polymerases Raffaele DE FRANCESCO, ICBM, Pomezia, Italy 17h00 Outbreak of poliomyelitis in the Dominican Republic and Haiti associated with circulating type 1 vaccine-derived poliovirus Cara BURNS, Center for Disease Control, Atlanta, USA 17h30 Coffee break 18h00 RNA capping as an antiviral target : structure of the dengue 2 New Guinea virus guanylyltransferase with bound ribavirin 5'-triphosphate Bruno CANARD, Université Aix Marseille, Marseille, France 18h15 Second generation alphavirus replicon vaccine vectors Silvia PERRI, Chiron Corporation, Emeryville, USA 18h45 Yellow fever vector live-virus vaccine against West Nile virus Juan ARROYO, Acambis Inc, Cambridge, USA 19h15 Recombinant hepatitis E virus-like particles as an oral vaccine Naokazu TAKEDA, National Institute of Infectious Diseases, Tokyo, Japan 19h30 End of the session Evening : Cruise on the Seine Saturday June 2nd, 2001 Morning PLENARY SESSION VI - HOST CELL RESPONSES TO VIRUS INFECTIONS Chairpersons : Margo BRINTON, Diane E. GRIFFIN 9h00 Membrane rearrangements, derangements and utilization during poliovirus infection Karla KIRKEGAARD, Stanford University School of Medicine, Stanford, USA 9h30 Cardiovirus protein 2A localizes to the nucleolus, stimulates rRNA synthesis, and modifies ribosomal subunits Ann PALMENBERG, University of Wisconsin, Madison, USA 10h00 Alphaviruses, apoptosis, and autophagy: new lessons about antiviral host defense Beth LEVINE, Columbia University, New-York, USA 10h30 Transcriptional regulation of MHC-I by flavivirus West-Nile is dependent upon NF-kB activation Alison M. KESSON, The Children’s Hospital at Westmead, Westmead, Australia 10h45 Coffee break 11h15 Poliovirus protein inhibits TNF-induced apoptosis by eliminating TNF receptor from the cell surface N. NEZNANOV, University of Illinois, Chicago, USA 11h45 IFN-inducible genes that regulate alphavirus virulence Herbert VIRGIN, Washington Univ. School of Medicine, St Louis, USA 12h15 Gene silencing as an antiviral defense system David BAULCOMBE, The Sainsbury Laboratory, Norwich, UK 12h45 Conclusions 13h00 End of the meeting