Under the patronage and sponsorship of

ANRS and OAR

8ème Colloque annuel sur la dynamique
et l'evolution du VIH

8th Annual International Discussion Meeting on HIV Dynamics and Evolution

27-29 Avril, 2001 / April 27-29, 2001

Paris, France

Le congrès commencera le matin du 27 Avril, 2001 et se terminera vers 17heures le 29 Avril. Un repas sera offert sur place tous les jours et un dîner sera organisé le premier soir accompagné d'un séminaire sur un thème d'actualité. Ceux qui ont participé les années précédentes savent que le nombre de participants à ce colloque est restreint à 130 personnes environ afin d'assurer des discussions animées sur l'évolution du virus et les approches dynamiques pour l'étude de la pathogenèse et la vaccinologie.

Les résumés doivent être envoyés par courrier électronique et par la poste à M. Peeters or MC Müller-Trutwin

The abstract should be submitted both electronically AND by correspondence. Send the abstract to M. Peeters or MC Müller-Trutwin :

Les résumés doivent inclure: titre, auteurs et adresses, objectifs, matériel et méthodes, résultats et conclusions et ne doivent pas dépasser les 400 mots.

The abstract should include : Title, authors and affiliations, a summary including background, objectives, materials and methods, results, and conclusions. Abstracts should not exceed 400 words.

Posters can be 150cm high and 90cm large.

1. Evolution and global variation of HIV and HIV related viruses
   chairmen: B.Hahn -M. Peeters
2. HIV evolution and transmission
   chairmen: M. Kalish - G. Scarlatti
3. HIV evolution and drug resistance
   chairmen: M. Wainberg -A.Leigh-Brown

4. Dynamics of non-pathogenic SIV infections in non-human primates.
   chairmen: M.Müller-Trutwin - M. Feinberg
5. Lessons from other viruses
   chairmen: A.Neumann - J.M.Pawlotsky
6. . Dynamic interactions between HIV and the immune system
   chairmen: B. Autran - A.McLean

7. Modeling of immunological parameters
   chairmen: B.Korber - G. Learn
8. Mathematical modeling of HIV evolution
   chairmen: J.Coffin - A. Vandamme
9. HIV dynamics in viral reservoirs
   chairmen: - D.Richman - A.Perelson

Final program

9.00-9.15   Kazatchkine M
ANRS, Paris, France
Opening Remarks

Session 1 : Evolution and global variation of HIV related viruses
Co-Chairs: B.Hahn, University of Alabama at Birmingham, USA
M.Peeters, IRD, Montpellier, France

9.15   Sharp P, University of Nottingham, UK
Cross-species transmission and evolution of HIV-1 and HIV-2   

9.40   Abela B, Projet PRESICA, Cameroon/IRD, Montpellier, France
Exposure of humans to SIVs in central Africa    

10.00   Tutin C, CIRMF, Gabon / University of Sterling, UK
The "wild card": What field studies can contribute to understanding the natural history and evolution of primate lentiviruses.

10.20-10.30 Discussion

10.30-11.00 BREAK

11.00   Roques P, CIRMF, Gabon
Wild Mandrillus sphinx are carriers of two types of lentiviruses   

11.20   Hirsch V, NIAID, NIH, Rockville, USA
Molecular Characterization of Simian Immunodeficiency Virus from Redcapped Mangabeys and Drills (SIVrcm and SIVdrl)
.
11.40   Clewley J, Central Public Health Laboratory, London, UK
Novel pol sequences from the Mona monkey, Cercopithecus mona, and de Brazza's monkey, C. neglectus

11.55   Saragosti S, INSERM U552/IMEA, Paris, France
Molecular characterization of primate lentiviruses from Cercopithecus wolfi and Cercopithecus ascanius.

12.10-12.20 Discussion

12.30-14.00 LUNCH

Session 2 : HIV evolution and transmission
Co-chairs: Kalish M, Centers for Disease Control and Prevention, Atlanta, USA
Scarlatti G, San Raffaelle Scientific Institute, Milan, Italy

14.00   Mullins J, University of Washington, Seattle,USA
Dual infection and Competitive Selection for Human Immunodeficiency Virus Type-1 Chimeras in vivo

14.20   Margolis L, NIH, Bethesda, USA
Evolution of co-receptor tropism and HIV pathogenesis in human lymphoid tissue.

14.40   Tscherning-Casper C, Karolinska Institute, Stockholm, Sweden
Evolution of co-receptor usage of HIV-1 isolates of different genetic subtypes in pregnant women and their infected children

15.00   König R, Paul Ehrlich Institut, Langen, Germany
Change of co-receptor usage of SIVagm3mc and consequences for its infection profile in vivo

15.20-15.30 Discussion

15.30-16.00 BREAK

session 3 : HIV evolution and drug resistance
co-chairs: Wainberg M, McGill University, Montreal, Canada
Leigh-Brown A, Univ of Edinburgh, Scotland/Univ of California, USA

16.00   Perrin L, Hôpital Cantonal Universitaire, Geneva, Switzerland
Transmission of HIV drug resistant variants.

16.20   Little S University of California, San Diego, USA
Prevalence and implications of reduced antiretroviral drug susceptibility among HIV infected subjects in North America

16.40   De Ronde T , AMC, Amsterdam, The Netherlands
Transmission, fitness, and losing control of HIV-1.

17.00   Martinez-Picado J, University Hospital "Germans Trias i Pujol", Badalona, Spain
Selection of drug resistant HIV-1 mutants in response to repeated structured treatment interruptions.

17.20   Grant R, University of California, San Fransisco, USA
Large Fitness Differences Between Protease Inhibitor Susceptible and Resistant HIV-1 in Vivo.

17.35   Frenkel L , University of Washington, Seattle, USA
Viral Evolution and Population Dynamics in Children on HAART with Plasma HIV-1 RNA levels <50 copies/ml.

17.50-18.00 Discussion

19.00-22.00 BANQUET DINER (Institut Pasteur) and SPECIAL LECTURE:   
Dormont D., CEA, France
Cross-species transmission and health risks for humans: the prion case

Saturday, 28 April 2001

Session 4 : Dynamics of non-pathogenic SIV infections in non-human primates
Co-chairs: Feinberg M, Emory University School of Medecine, Atlanta, USA
Müller-Trutwin M C, Institut Pasteur, France

9.00   Verschoor E, TNO, Rijswijk, The Netherlands
Routes of transmission of SIVcpz and kinetics of infection in the presence and absence of concurrent HIV-1 infection in chimpanzees

9.20   Onanga R , CIRMF,Gabon
SIVmnd-1 In Mandrillus sphinx: A New Model for Non -Pathogenic SIV Infection

9.30    Kornfeld C, Institut Pasteur, Paris, France
Early high level replication of SIVagm in AGMs is associated with transient activation of peripheral and lymph node T cells

9.40   Feinberg M , Emory University School of Medecine, Atlanta, USA
Ignorance is Bliss: How sooty mangabeys remain healthy despite SIV infection

10.00   Estaquier J , INSERM, Paris, France
T cell turnover and apoptosis in SIV-infected macaques.

10.15-10.30 Discussion

10.30-11.00 BREAK

session 5 : lessons from other viruses
Co-Chairs: Neuman A , Bar-Ilan University , Ramat-Gan, Israel)
Pawlotsky JM, Hopital Henri Mondor, Paris, France)

11.00    Osterhaus A, Erasmus University, Rotterdam, The Netherlands
Animal models of viral infection

11.20   Bray M , Fort Detrick, Frederick , USA
Dynamics of Ebola virus infection in experimental animals

11.40   Rice C, The Rockefeller University , New York, USA)
Advances in hepatitis C: Chimpanzee studies and replication in cell culture.

12.00   Dahari    D , Bar-Ilan University , Ramat-Gan, Israel
Modeling Primary HCV Infection in Chimpanzees

12.20-12.30 Discussion

12.30-14.00 LUNCH

session 6 : Dynamic interactions between HIV and the immune system
Co-chairs: Autran B , CH Pitie Salpetriere, Paris, France)
McLean A, Oxford University, UK

14.00   Shaw G University of Alabama at Birmingham, USA
Role of sequential epitope change, viral recombination, and reactivation of latent proviral exression in HIV-1 persistence and CTL escape

14.20   De Boer R, University of Utrecht, The Netherlands
Estimating the average cellular turn-over from BrdU experiments

14.40   Frost S, University of California, SanDiego , USA
Viral and immune dynamics during structured therapy interruptions of HIV-1

15.00   Oxenius A, John Radcliffe Hospital Oxford UK
Structured treatment interruption: Impact on HIV-specific immunity

15.20-15.30 Discussion

15.30-16.00 BREAK

16.00    Staprans S , Emory University, Atlanta, USA
CD4 and CD8 T cell dynamics during primary SIV infection and their relationship to virus replication.

16.20   Korthals H, CH Pitié-Salpétrière, Paris, France)
Adjuvant scIL2 increases thymic production in patients with advanced HIV infection under antiretroviral therapy.

16.35-16.45 Discussion

16.45-17.30 Round table : Dynamics of host immune cells
B. Autran (France), N.Israel (France), A.McLean (UK),
F. Miedema (The Netherlands), B. Roche (France)

POSTER SESSION

COCKTAIL

Sunday, 29 April 2001

session 7 : HIV variation, immune recognition and escape
Co-chairs: B.Korber , Santa Fe Institute/Los Alamos National Institute, USA
G. Learn , University of Washington, Seattle, USA

9.00   Wain-Hobson S, Institut Pasteur, Paris, France
Dynamic escape of SIV from immune responses

9.20   Goulder P , John Radcliffe Hospital, Oxford UK
Transmission of CTL escape mutations in HIV infection

9.40   Watkins D, Wisconsin Regional Primate Research Center, Madison USA
SIV Escape Variants Selected By CTL

10.00   Korber B, Santa Fe Institute/Los Alamos National Institute, USA
HIV variation and CTL processing and neutralizing antibody escape

10.15   Wyatt R, Dana-Farber cancer Institute,Boston, USA
HIV-1 gp120 "Core" Glycoproteins Deficient in T Cell Helper Epitopes

10.30-11.0 BREAK

session 8 : Mathematical modeling of HIV evolution
Co-chairs: Coffin J, NCI, Frederick, USA
Vandamme A, University of Leuven, Leuven, Belgium

11.00   Yang Z , University College London, UK
Detecting adaptive evolution in viral genes

11.20   Wakely John, Harvard University, Cambridge, USA
A Metapopulation Coalescent Model for HIV Sequence Data

11.40   Rouzine I , Tufts University , Boston, USA
Evolution of many linked loci in the presence of selection: Muller's ratchet as a solitary wave in haplotype space

11.55   Lemey P , Universisty of Leuven, Belgium
Maximum likelihood analysis of clock-like evolution in HIV-1 group M
incorporating non-contemporaneous sequences.

12.10   Karina Yusim, Los Alamos National Laboratory, USA
Using HIV-1 Sequences to Infer Historical Features of the AIDS Epidemic in the Democratic Republic of Congo.

12.25-12.40 Discussion

12.40-14.00 LUNCH

session 9 : HIV dynamics in viral reservoirs
Co-chairs: Richman D, University of California San Diego, USA
Perelson A, Los Alamos National Laboratory, USA

14.00   Persaud    D , Johns Hopkins University School of Medicine, Baltimore , USA
HIV-1 in latent cellular reservoirs and plasma of infected individuals with less than 50 copies/ml of plasma HIV-1 RNA during combination antiretroviral therapy exhibits a static, archival pattern of viral persistence.

14.20   Wong Joe , University of California San Diego, USA
Distinguishing slow intrinsic decay rate and replenishment of latently infected CD4 cells in patients with long term suppression of viremia.

14.40   Strain M, University of California San Diego, USA
Stochastic interaction with replication sanctuaries decreases clearance of the latent reservoir

15.00-15.15 Discussion

15.15-16.00 Round Table "viral reservoirs "
D.Richman (USA) - M. Wainberg(Canada)
F.Clavel (France) -S.Wolinsky (USA) - J.Coffin(USA) -

16.00 COFFEE

Le congrès commencera le matin du 27 Avril, 2001 et se terminera vers 17heures le 29 Avril. Un repas sera offert sur place tous les jours et un dîner sera organisé le premier soir accompagné d'un séminaire sur un thème d'actualité.

The meeting will begin on Friday morning, April 27, 2001 and conclude on Sunday early afternoon, April 29, 2001. A lunch will be provided daily to all paid registrants. There will be a diner on Friday evening.

Reservez votre hotel auprès de Monique Saltel (voir aussi formulaire d'enregistrement). For making your reservations, join:

Ceux qui ont participé les années précédentes savent que le nombre de participants à ce colloque est restreint à 130 personnes environ afin d'assurer des discussions animées. Ce chiffre a été atteint et dès aujourd'hui les inscriptions seront prioritaires pour les personnes dont l'abstract a été accepté pour présentation (poster ou oral), les autres nouvelles inscriptions seront mises sur une liste d'attente. La réponse sera envoyée avant le 6 Avril.

The number of participants has reached 130 persons. Those of you who have attended previous meetings in this series know that the number of participants is restricted in order to garantee lively discussions. From now on registrations are in priority for individuals with an abstract accepted as poster or oral presentation, for all other new inscriptions a waiting list will be established and a final response about acceptance will be send before April 6.

• By train: take the airport shuttle to the railway station "Gare TGV-RER" and then RER B to Denfert-Rochereau (each 15 min), then taxi or metro line 6.
• By bus: take the Air France buses "Les cars Air France" to Montparnasse (each 30 min, about 50 min trip, 70FF)

• By train: take Orlyval (each 5 min) to Antony and then RER B to Denfert-Rochereau (each 10 min), then taxi or metro line 6.
• By bus: take the Air France buses "Les cars Air France" to Montparnasse (each 12 min, about 30 min trip, 45FF)