2nd International Meeting & Workshop on Advanced Light Microscopy

May 29-31, 2002
Institut Pasteur, Paris, France
Amphithéâtre Duclaux



 
Abstract submission deadline extended to April 11th 2002

 


  Ulf Nehrbass, Institut Pasteur, Paris (France)
Jean-Christophe Olivo-Marin, Institut Pasteur, Paris (France)
Spencer Shorte, Institut Pasteur, Paris (France)


 


  Applied Precision, LLC, USA
BitPlane AG, Switzerland / SGI, France
Evotec Technologies, Germany
Hamamatsu Photonics, France
Leica Microsystems Heidelberg GmbH, Germany
Olympus Optical Co. (Europa) GmbH, Germany
Till Photonics GmbH, Germany
Zeiss, France


 


  Wednesday 29th May 2002
Chair: Ulf Nehrbass


8:30 - 9:15 Registration

9:15 - 9:30 Alain Gouyette
Associate Director General, Institut Pasteur, Paris Welcome Address

9:30 - 10:10 John Sedat, Bridget Hanser, Lin Shao, Mats Gustafsson and David Agard
Department of Biochemistry and Biophysics, University of California, San Francisco, USA
Frontiers in Wide-Field Microscopy: Phase Retrieval, Aberration Control, and Structured Illumination High-Resolution Technology

10:10 - 10:50 Jason R. Swedlow, Melpomeni Platani, Angus I. Lamond, Ilya Goldberg*, Peter Sorger*
Division of Gene Regulation and Expression MSI/WTB Complex, University of Dundee, Scotland
*Dept of Biology, Massachusetts Institute of Technology, Cambridge, USA
I. An analysis of cajal body dynamics in living cells
II. An open image informatics solution for biological microscopy


10:50 - 11:20 Coffee Break

11:20 - 11:40 Peter J. Verveer1, Fawaz G. Haj2, Anthony Squire1, Benjamin G. Neel2, and Philippe I. H. Bastiaens1
1European Molecular Biology Laboratory, Heidelberg, Germany
2Harvard Institutes of Medicine, Boston USA
Imaging Sites of Receptor Dephosphorylation by PTP1B on the Surface of the Endoplasmic Reticulum

11:40 - 12:00 J.-B. Sibarita (1), H. Magnin (2), J.R. De Mey (3)
CNRS, Institut Curie, Research Section
Real time deconvolution applied to ultra-fast 4d microscopy: soon a reality ?

12:00 - 12:20 Ginette Ploger, Birgit Staehr, Graciela Humbert-Lan, and Donna J. Arndt-Jovin
Dept. of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany
Nuclear Organization in Drosophila. Dynamic changes during Development


12:20 - 12.40 Pascal Vallotton, Aaron Ponti, Clare Waterman-Storer, Gaudenz Danuser
Laboratory for Biomechanics, Department for Mechanical and Process Engineering Swiss Federal Institute of Technology, ETHZ, Schlieren, Switzerland
Polymerisation/depolymerisation, and retrograde flow in fluorescence speckle microscopy (FSM)

12:40 - 13:00 Christophe Zimmer (*), Elisabeth Labruyère (%), Vannary Meas-Yedid (*), Estelle Glory (*), Nancy Guillén (%), Jean-Christophe Olivo-Marin (*)
(*) Unité d'Analyse d'Images Quantitative, Institut Pasteur, Paris
(%) Unité de Biologie Cellulaire du Parasitisme, Institut Pasteur, Paris
Segmentation and tracking of migrating cells in videomicroscopy using adapted active contours

13:00 - 14:00 Lunch & Poster Session

14:00 - 18:00 Workshops


18:00 - 18:40 George H. Patterson & Jennifer Lippincott-Schwartz
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA
A new photoactivatable GFP with greatly enhanced optical contrast for selective photolabeling of proteins and cells in vivo

18:40 - 19:20 C. Cremer (1)*, B. Albrecht (1), G. Kreth (1), V. Failla (1), Schweitzer (1), A. Cavallo (1), H. Eipel (1), J. von Hase (1), J. Rauch (1), N. Kepper (1), J. Finsterle(1), S. Stein (1), M. Hausmann (1), L. Trakthenbrot (2), Solovei (3), L. Schermelleh (3), M. Cremer (3), T. Cremer (3)
(1)Kirchhoff-Institut für Physik, Universität Heidelberg
(2) Institut for Hematology, The Chaim Sheba Medical Centre, Tel Hashomer, Israel
(3) Institut für Anthropologie und Humangenetik, Ludwig Maximilian Universität, München, Germany
Nanotopology of Nuclear Genome Structure

19:30 Cocktail

Thursday, 30th May 2002
Chair: Jean-Christophe Olivo-Marin


8:30 - 9:10 I.T. Young, R. van den Doel, L.J. van Vliet, G. van Dedem, R. Moerman, A. Bossche, V. Iordanov
Delft University of Technology
Microarrays & Microscopes: A marriage made in silicon

9:10 - 9:50 Ramiro Massol, Aster Leguesse-Miller, Jan Cerny, Marianne Boes, Hidde Plough, Tom Kirchhausen
Department of Cell Biology and The Center for Blood Research, Harvard Medical School, USA
Membrane traffic using time-resolved confocal and deconvolution microscopy

9:50 - 10:30 Zvi Kam
Molecular Biology of the Cell, Weizmann Institute of Science, Rehovot, Israel
Microscoping in post-genomic cell biology

10:30 - 11:00 Coffee Break

11:00 - 11:40 Mark A. McNiven
Mayo Clinic and Foundation, Rochester, USA
The Large GTPase Dynamin as a Mediator of Dynamic Actin-Membrane Interactions

11:40 - 12:00 Sylvie Coscoy1, François Waharte1, Claire Brown1, Evelyne Coudrier2, Monique Arpin2, Paul Mangeat3, Daniel Louvard2 and François Amblard1
CNRS-UMR-168(1) and UMR-144(2), Institut Curie, Paris (3) France
CNRS-UMR-5539, Université de Montpellier, Montpellier, France
Microscopic dynamics of an intracellular morphogen : the ezrin-actin skeleton studied by two-photon frap

12:00 - 12:20 Kota Miura, Jens Rietdorf & Rainer Pepperkok
Cell Biology and Biophysics Programme, EMBL Heidelberg, Germany
A Gradient Method for the Quantitative Analysis of Cell and Organelle Motility

12:20 - 12:40 Anne Débarre, Rodolphe Jaffiol, Carine Julien, Daniele Nutarelli, P. Tchénio
Laboratoire Aimé Cotton, Orsay, France
A nanometric probe of the environment : the single molecule

12:40 - 13:00 Beznoussenko G. V., A.A. Mironov, Jr., Luini A. and A.A. Mironov
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud. S. Maria Imbaro (Chieti), Italy
Analysis of intracellular transport with video-light-electron microscopy

13:00 - 14:00 Lunch & Poster Session

14:00 - 18:00 Workshops


18:00 - 18:40 Tom Jovin
Dept. of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
New Approaches To Fret Microscopy: emFret and pcFret

18:40 - 19:20 Pascale Cossart
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France
Actin rearrangements during internalization of Listeria monocytogenes

19:30 Departure for the Gala dinner

Friday 31st May 2002
Chair: Spencer Shorte

8:30 - 9:10 G.J. Brakenhoff
Univ. of Amsterdam, Institute for Molecular Cell Biology Kruislaan, Amsterdam, The Netherlands
Sectioned imaging in microscopy

9:10 - 9:50 Thierry Laroche, Florence Hediger, Frank R. Neumann, Griet van Houwe, Angela Taddei and Susan M. Gasser
University of Geneva, Department of Molecular Biology, Geneva, Switzerland
3D and 4D imaging of budding yeast chromosomes: yKu and Sir proteins anchor telomeres in a redundant fashion

9:50 - 10:30 Serge Charpak
EPI 0002 Inserm, ESPCI, Paris, France
Two photon imaging of neuronal activity and vascular flow in the rat olfactory bulb

10:30 - 11:00 Coffee Break

11:00 - 11:40 Karine Monier, Astrid E. Visser, Samantha G. Zeitlin and Kevin F. Sullivan
Department of Cell Biology, The Scripps Research Institute, La Jolla, USA
Assembly and modification of centromeric chromatin in the cell cycle

11:40 - 12:00 Olivier Gadal1#, Vincent Galy 1,4#, Micheline Fromont-Racine 2, Katja Strasser3, Ed Hurt 3 Alain Jacquier 2 and Ulf Nehrbass 1*
1 Unité de Biologie Cellulaire du Noyau, CNRS URA 1773, France
2 Unité de Génétique des Interaction Macromoléculaire, Paris, France
3 BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, Heidelberg, Germany
A Perinuclear Subdomain is Required for Nuclear Retention of Unspliced mRNA

12:00 - 12:20 T. Piolot §*, M. Tramier §, J.-C. Mével §, J. Coppey §, K. Kemnitz * M. Coppey-Moisan § and C. Durieux §
§ Institut Jacques Monod, UMR 7592, CNRS, Universités P 6/7, Paris, France
EuroPhoton GmbH, Berlin, Germany
Picosecond-FRET imaging in living cells: protein-protein and protein-DNA interactions

12:20 - 12:40 Régis Grailhe*, Hélène Laguitton-Pasquier, Stephen Couvignou, Marie-Pierre Fontaine-Aupart, Patrick Georges, Bernard Arrio, Jean-Pierre Changeux*, Fabienne Mérola
*Institut Pasteur, Laboratoire Récepteurs et Cognition, Paris, France
Laboratoire de Chimie Physique, Université Paris-Sud, Orsay, France
Protein interactions by means of Fluorescence Lifetime Imaging Microscopy using biphotonic excitation in living cells

12:40 - 13:00 Manuela Matos, Nawrocki-Raby Béatrice, Zahm Jean-Marie, Polette Myriam, Birembaut Philippe, Bonnet Noël
INSERM UMRS514, IFR53, CHU Maison-Blanche, Reims cedex, France
Videomicroscopy, cellular sociology and tumour Invasion

13:00 - 14:00 Lunch & Poster Session

14:00 - 18:00 Workshops


18:00 - 18:40 Andrés Alcover
Unité de Biologie des Interactions Cellulaires, CNRS URA 1960, Institut Pasteur, Paris, France
Membrane-cytoskeleton interactions during the formation of the "immunological synapse"

18:40 - 19:20 Andrew S. Belmont
Dept. of Cell and Structural Biology, University of Illinois, Urbana USA

Site web of ELMI : http://www.embl-heidelberg.de/ELMI


 


  Abstracts will be selected for either oral or poster presentation, at the discretion of the Organising Committee. The decision will be communicated by mail after April 15th, 2002.
Please send your abstract by e-mail (Word or RTF document) to idulieu@pasteur.fr before March 31st, 2002 mentioning: title, name of presenting author underlined, institution. These headlines plus the abstract of 300 words maximum must fit in an A4 frame (21 x 29,7 cm).

Posters: The maximum poster size must be of 0.90 m high on 1.20 m wide. Velcro (given on site) will be used to fix it to the board. Posters will be exposed throughout the Meeting.


 


 


  Please fill in the registration form and return it before March 31st, 2002 to the Institut Pasteur together with your payment in order to have advantage of registration fee proposed up to this date. Confirmation of registration and receipt of payment will be sent after April 15th, 2002.
Abstracts sent before March 31st, 2002 will allow delegates presenting abstract to register at the fee proposed up to this date.


 


  Registration fee covers cost for the following: admission to the scientific sessions, coffee breaks, lunches, welcome cocktail, gala dinner, abstract book and final program.

Fees (VAT 19,6% included):
Before March 31st, 2002
Delegates 330 Euros
Students: 200 Euros
Accompanying person (participation at the welcome cocktail and Gala dinner): 80 Euros

After March 31st, 2002
Delegates 400 Euros
Students: 250 Euros
Accompanying person (participation at the welcome cocktail and Gala dinner): 80 Euros
Institut Pasteur : see conditions


 


  Hotel rooms at special rates have been reserved from May 28th to June 1st in hotels mainly located next to the Pasteur Institute. Their capacities being limited, it is highly recommended to book a room as soon as possible. In order to make a reservation, please fill in the form and return it to CARLSON WAGONLIT EVENEMENTS (see contact details on the form).



 


  Wednesday 29 May: Welcome cocktail
Thursday 30 May: Gala diner


 


  A refund of the registration fee, less a service charge of 50 Euros, will be made in respect of a written notification of cancellation received no later than April 30th, 2002; thereafter no refunds will be made. A substitute participant is welcome at no extra charge before April 30th, 2002. Please note that all refunds will be issued after the Conference.


 


  Venue
Institut Pasteur
Grand Amphithéâtre
28 rue du Docteur Roux – 75015 Paris, France

Ground transportation: see « practical information »

Average temperatures in Paris in May-June: 20 °C

Dress: informal on all occasions


 


  Institut Pasteur
Centre d’Information Scientifique – Gestion
28 rue du Docteur Roux – 75724 Paris cedex 15, France
Fax: + 33 (0)1 40 61 34 05 - Email: colloque@pasteur.fr