Rapid tests for diagnosis of leptospirosis: current tools and emerging technologies.

Picardeau M, Bertherat E, Jancloes M, Skouloudis AN, Durski K, Hartskeerl RA.

Diagn Microbiol Infect Dis. 2013 Oct 1.


Structural and Functional Characterization of an Orphan ATP-Binding Cassette ATPase Involved in Manganese Utilization and Tolerance in Leptospira spp.

Benaroudj N, Saul F, Bellalou J, Miras I, Weber P, Bondet V, Murray GL, Adler B, Ristow P, Louvel H, Haouz A, Picardeau M.

J Bacteriol. 2013 Dec;195(24):5583-91.


Sensitivity and Specificity of a New Vertical Flow Rapid Diagnostic Test for the Serodiagnosis of Human Leptospirosis.

Goarant C, Bourhy P, D'Ortenzio E, Dartevelle S, Mauron C, Soupé-Gilbert ME, Bruyère-Ostells L, Gourinat AC, Picardeau M, Nato F, Chanteau S.

PLoS Negl Trop Dis. 2013 Jun 27;7(6):e2289.


Leptospirosis in the western Indian Ocean islands: what is known so far?

Desvars A, Michault A, Bourhy P.

Vet Res. 2013 Sep 9;44(1):80.


Outbreak of leptospirosis among canyoning participants, Martinique, 2011.

Hochedez P, Escher M, Decoussy H, Pasgrimaud L, Martinez R, Rosine J, Théodose R, Bourhy P, Picardeau M, Olive C, Ledrans M, Cabie A.

Euro Surveill. 2013 May 2;18(18):20472.


Precipitation of iron on the surface of Leptospira interrogans is associated with mutation of the stress response metalloprotease HtpX.

Henry R, Lo M, Khoo C, Zhang H, Boysen RI, Picardeau M, Murray GL, Bulach DM, Adler B.

Appl Environ Microbiol. 2013 Aug;79(15):4653-60.


Leptospiral outer membrane protein LipL41 is not essential for acute leptospirosis but requires a small chaperone protein, lep, for stable expression.

King AM, Bartpho T, Sermswan RW, Bulach DM, Eshghi A, Picardeau M, Adler B, Murray GL.

Infect Immun. 2013 Aug;81(8):2768-76.


Serovar diversity of pathogenic Leptospira circulating in the French West Indies.

Bourhy P, Herrmann Storck C, Theodose R, Olive C, Nicolas M, Hochedez P, Lamaury I, Zinini F, Brémont S, Landier A, Cassadou S, Rosine J, Picardeau M.

PLoS Negl Trop Dis. 2013;7(3):e2114.


Evaluation of an in-house ELISA using the intermediate species Leptospira fainei for diagnosis of leptospirosis.

Bourhy P, Vray M, Picardeau M.

J Med Microbiol. 2013 Jun;62(Pt 6):822-7.


Diagnosis and epidemiology of leptospirosis.

Picardeau M.

Med Mal Infect. 2013 Jan;43(1):1-9.


Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa.

Stewart PE, Carroll JA, Dorward DW, Stone HH, Sarkar A, Picardeau M, Rosa PA.

BMC Microbiol. 2012 Dec 13;12:290. doi: 10.1186/1471-2180-12-290.


Chemotactic behavior of pathogenic and nonpathogenic Leptospira species.

Lambert A, Takahashi N, Charon NW, Picardeau M.

Appl Environ Microbiol. 2012 Dec;78(23):8467-9.


Leptospira interrogans catalase is required for resistance to H2O2 and for virulence.

Eshghi A, Lourdault K, Murray GL, Bartpho T, Sermswan RW, Picardeau M, Adler B, Snarr B, Zuerner RL, Cameron CE.

Infect Immun. 2012 Nov;80(11):3892-9.


Similarities in Leptospira serogroup and species distribution in animals and humans in the Indian ocean island of Mayotte.

Desvars A, Naze F, Vourc'h G, Cardinale E, Picardeau M, Michault A, Bourhy P.

Am J Trop Med Hyg. 2012 Jul;87(1):134-40.


FlaA proteins in Leptospira interrogans are essential for motility and virulence but are not required for formation of the flagellum sheath.

Lambert A, Picardeau M, Haake DA, Sermswan RW, Srikram A, Adler B, Murray GA.

Infect Immun. 2012 Jun;80(6):2019-25.


La leptospirose

Bourhy P, Hochedez P, Picardeau M

EMC-Maladies Infectieuses 2012 Feb; 9:1-12.


Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon.

Slamti L, Picardeau M.

Methods Mol Biol. 2012;859:169-76.


Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features.

Bourhy P, Collet L, Lernout T, Zinini F, Hartskeerl RA, van der Linden H, Thiberge JM, Diancourt L, Brisse S, Giry C, Pettinelli F, Picardeau M.

J Clin Microbiol. 2012 Feb;50(2):307-11.


Deciphering morphological determinants of the helix-shaped Leptospira.

Slamti L, de Pedro MA, Guichet E, Picardeau M.

J Bacteriol. 2011 Nov;193(22):6266-75.


Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions.

Lourdault K, Cerqueira GM, Wunder EA Jr, Picardeau M.

Infect Immun. 2011 Sep;79(9):3711-7.


Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.

Figueira CP, Croda J, Choy HA, Haake DA, Reis MG, Ko AI, Picardeau M.

BMC Microbiol. 2011 Jun 9;11:129.


Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences.

Bourhy P, Bremont S, Zinini F, Giry C, Picardeau M.

J Clin Microbiol. 2011 Jun;49(6):2154-60.


Outbreak of leptospirosis after a race in the tropical forest of Martinique.

Hochedez P, Rosine J, Théodose R, Abel S, Bourhy P, Picardeau M, Quénel P, Cabié A.

Am J Trop Med Hyg. 2011 Apr;84(4):621-6.


The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.

Choy HA, Kelley MM, Croda J, Matsunaga J, Babbitt JT, Ko AI, Picardeau M, Haake DA.

PLoS One. 2011 Feb 9;6(2):e16879.


Expanding the genetic toolbox for Leptospira species by generation of fluorescent bacteria.

Aviat F, Slamti L, Cerqueira GM, Lourdault K, Picardeau M.

Appl Environ Microbiol. 2010 Dec;76(24):8135-42.


Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean).

Bourhy P, Collet L, Clément S, Huerre M, Ave P, Giry C, Pettinelli F, Picardeau M.

PLoS Negl Trop Dis. 2010 Jun 22;4(6):e724.


Antibiotic resistance markers for genetic manipulations of Leptospira spp.

Poggi D, Oliveira de Giuseppe P, Picardeau M.

Appl Environ Microbiol. 2010 Jul;76(14):4882-5.


Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen.

Ko AI, Goarant C, Picardeau M.

Nat Rev Microbiol. 2009 Oct;7(10):736-47.


TLR4- and TLR2-mediated B cell responses control the clearance of the bacterial pathogen, Leptospira interrogans.

Chassin C, Picardeau M, Goujon JM, Bourhy P, Quellard N, Darche S, Badell E, d'Andon MF, Winter N, Lacroix-Lamandé S, Buzoni-Gatel D, Vandewalle A, Werts C.

J Immunol. 2009 Aug 15;183(4):2669-77


A century of Leptospira strain typing.

Cerqueira GM, Picardeau M.

Infect Genet Evol. 2009 Sep;9(5):760-8.


Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.

Cerqueira GM, McBride AJ, Picardeau M, Ribeiro SG, Moreira AN, Morel V, Reis MG, Ko AI, Dellagostin OA.

J Med Microbiol. 2009 Sep;58(Pt 9):1173-81.


Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis.

Lourdault K, Aviat F, Picardeau M.

J Med Microbiol. 2009 May;58(Pt 5):648-55.


Evolution and pathogenesis of Leptospira spp.: lessons learned from the genomes.

Xue F, Yan J, Picardeau M.

Microbes Infect. 2009 Mar;11(3):328-33.


[Alveolar haemorrhage in leptospirosis].

Cottin V, Picardeau M, Cordier JF.

Rev Prat. 2008 Nov 15;58(17):1869. French.


Genome-wide transposon mutagenesis in pathogenic Leptospira species.

Murray GL, Morel V, Cerqueira GM, Croda J, Srikram A, Henry R, Ko AI, Dellagostin OA, Bulach DM, Sermswan RW, Adler B, Picardeau M.

Infect Immun. 2009 Feb;77(2):810-6.


Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis.

Croda J, Figueira CP, Wunder EA Jr, Santos CS, Reis MG, Ko AI, Picardeau M.

Infect Immun. 2008 Dec;76(12):5826-33.


Genetic manipulation of Leptospira biflexa.

Louvel H, Picardeau M.

Curr Protoc Microbiol. 2007 Jun;Chapter 12:Unit 12E.4.


Biofilm formation by saprophytic and pathogenic leptospires.

Ristow P, Bourhy P, Kerneis S, Schmitt C, Prevost MC, Lilenbaum W, Picardeau M.

Microbiology. 2008 May;154(Pt 5):1309-17.


Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis.

Picardeau M, Bulach DM, Bouchier C, Zuerner RL, Zidane N, Wilson PJ, Creno S, Kuczek ES, Bommezzadri S, Davis JC, McGrath A, Johnson MJ, Boursaux-Eude C, Seemann T, Rouy Z, Coppel RL, Rood JI, Lajus A, Davies JK, Médigue C, Adler B.

PLoS One. 2008 Feb 13;3(2):e1607.


Heme rescues a two-component system Leptospira biflexa mutant.

Louvel H, Betton JM, Picardeau M.

BMC Microbiol. 2008 Jan 30;8:25.


Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool.

Picardeau M.

Appl Environ Microbiol. 2008 Jan;74(1):319-22.


The OmpA-like protein Loa22 is essential for leptospiral virulence.

Ristow P, Bourhy P, da Cruz McBride FW, Figueira CP, Huerre M, Ave P, Girons IS, Ko AI, Picardeau M.

PLoS Pathog. 2007 Jul;3(7):e97.


A comprehensive survey on isoleucine biosynthesis pathways in seven epidemic Leptospira interrogans reference strains of China.

Zou Y, Guo X, Picardeau M, Xu H, Zhao G.

FEMS Microbiol Lett. 2007 Apr;269(1):90-6.


A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome.

Bourhy P, Salaün L, Lajus A, Médigue C, Boursaux-Eude C, Picardeau M.

Infect Immun. 2007 Feb;75(2):677-83.


Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis.

Salaün L, Mérien F, Gurianova S, Baranton G, Picardeau M.

J Clin Microbiol. 2006 Nov;44(11):3954-62.


Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp.

Louvel H, Bommezzadri S, Zidane N, Boursaux-Eude C, Creno S, Magnier A, Rouy Z, Médigue C, Saint Girons I, Bouchier C, Picardeau M.

J Bacteriol. 2006 Nov;188(22):7893-904.


The scc spirochetal coiled-coil protein forms helix-like filaments and binds to nucleic acids generating nucleoprotein structures.

Mazouni K, Pehau-Arnaudet G, England P, Bourhy P, Saint Girons I, Picardeau M.

J Bacteriol. 2006 Jan;188(2):469-76.


Complete nucleotide sequence of the LE1 prophage from the spirochete Leptospira biflexa and characterization of its replication and partition functions.

Bourhy P, Frangeul L, Couvé E, Glaser P, Saint Girons I, Picardeau M.

J Bacteriol. 2005 Jun;187(12):3931-40.


Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.

Bourhy P, Louvel H, Saint Girons I, Picardeau M.

J Bacteriol. 2005 May;187(9):3255-8.


Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis.

Louvel H, Saint Girons I, Picardeau M.

J Bacteriol. 2005 May;187(9):3249-54.


Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto.

Majed Z, Bellenger E, Postic D, Pourcel C, Baranton G, Picardeau M.

J Clin Microbiol. 2005 Feb;43(2):539-45.


The spirochetal chpK-chromosomal toxin-antitoxin locus induces growth inhibition of yeast and mycobacteria.

Picardeau M, Le Dantec C, Richard GF, Saint Girons I.

FEMS Microbiol Lett. 2003 Dec 12;229(2):277-81.


Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp.

Picardeau M, Bauby H, Saint Girons I.

FEMS Microbiol Lett. 2003 Aug 29;225(2):257-62.


Leptospira spp. possess a complete haem biosynthetic pathway and are able to use exogenous haem sources.

Guégan R, Camadro JM, Saint Girons I, Picardeau M.

Mol Microbiol. 2003 Aug;49(3):745-54.


Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri.

Bauby H, Saint Girons I, Picardeau M.

Microbiology. 2003 Mar;149(Pt 3):689-93.


Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology.

Tchamedeu Kameni AP, Couture-Tosi E, Saint-Girons I, Picardeau M.

J Bacteriol. 2002 Jan;184(2):452-8.


Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli.

Picardeau M, Ren S, Saint Girons I.

J Bacteriol. 2001 Nov;183(21):6494-7.


Erp, an extracellular protein family specific to mycobacteria.

de Mendonça-Lima L, Picardeau M, Raynaud C, Rauzier J, de la Salmonière YO, Barker L, Bigi F, Cataldi A, Gicquel B, Reyrat JM.

Microbiology. 2001 Aug;147(Pt 8):2315-20.


First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella.

Picardeau M, Brenot A, Saint Girons I.

Mol Microbiol. 2001 Apr;40(1):189-99.


Genomic sequence and transcriptional analysis of a 23-kilobase mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems.

Le Dantec C, Winter N, Gicquel B, Vincent V, Picardeau M.

J Bacteriol. 2001 Apr;183(7):2157-64.


The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector.

Girons IS, Bourhy P, Ottone C, Picardeau M, Yelton D, Hendrix RW, Glaser P, Charon N.

J Bacteriol. 2000 Oct;182(20):5700-5.

Mis à jour le 15/04/2014


Biologie des Spirochètes
Institut Pasteur
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