Legionella pneumophila nucleomodulins: characterization and identification of their targets in the host

A postdoctoral position*, funded by the Laboratory of Excellence Integrative Biology of Emerging Infectious Diseases (LabEx IBEID: http://www.pasteur.fr/labex/ibeid), is available in the "Biology of Intracellular Bacteria" research unit directed by Carmen Buchrieser at the Institut Pasteur in Paris.



Legionella pneumophila nucleomodulins: characterization and identification of their targets in the host


Understanding how pathogens induce chromatin modifications to affect their host is currently an emerging topic. Similar to viruses, bacteria may act in the nucleus by translocating genetic or epigenetic modulating
factors, recently called "nucleomodulins".
Using various tools we aim to identify and functionally characterize L. pneumophila proteins that target the host nucleus (nucleomodulins) and that are predicted to encode specific enzymatic activities. Recently we have identified and characterized the translocated effector RomA and have shown that RomA indeed targets the nucleus of the cell it infects and that it possesses specific methyltransferase activity against histone H3. We have now identified other Legionella effectors that encode a nuclear localization signal and are thus predicted to target the nucleus.
The aim of this project is to decipher the function of these newly identified, putative nuclear effectors and to characterize the host response induced by these Legionella nucleomodulins. Our long-term goal is to understand the global impact of nuclear modulations exerted by L. pneumophila during infection.

 

Candidate requirements:

The successful candidate is expected to have a solid background in molecular biology and an expertise knowledge in cellular biology. Knowledge on host-pathogen interactions and eukaryotic signaling pathways involved in host-pathogen interactions is highly welcomed.

 

Requirements:

PhD in relevant field

- Appropriate experience in research and high motivation

- Excellent scientific, organizational skills, reliable person

- Initiative and independent work abilities

- Excellent interpersonal and scientific communication skills, able to work in collaboration

- Good English language skills for presentations and writing scientific papers

 

To apply:

 

Applicants should send their CV, a motivation letter and 3 references to Carmen Buchrieser (carmen.buchrieser@pasteur.fr) Unité de Biologie des Bactéries intracellulaires, Institut Pasteur, 28 rue du Dr Roux 75015 Paris

Mis à jour le 15/11/2013