Pasteur course : Principles and Applications of Fluorescence Microscopy

This course is intended for Master’s students and international PhD students who expect to be using advanced live cell fluorescence microscopy for future studies.


The use of fluorescence microscopy (wide field, confocal, multiphoton, and now superresolution) in combination with genetically encoded fluorescence probes comprise a powerful set of scientific tools to study live cells. However, surprising little practical and theoretical training in such methods exists within standard curricula, particular at the early stage of training (Masters or Doctorate level). This course offers to cover basic optics principles necessary to understand the origin of microscope resolution and design. Participants will get hands-on experience implementing simple optical configurations to illustrate these fundamental principles.  Subsequently, participants will perform experiments on state-of-the-art imaging equipment provided by microscope vendors. Students will also learn basic principles of image analysis. Lectures will cover, in depth, the principle behind traditional high resolution imaging methods such as confocal, multi-photon, and the recently developed super-resolutions methods. Students will also learn about fundamental properties of synthetic and genetically encoded fluorescence indicators used for cellular morphology imaging and signaling recording. Finally guest lectures will demonstrate applications of some of the discussed methodologies.



Program of the course (for detailed information on the content of the course)



Members of the Teaching Committee:

David DiGregorio (Institut Pasteur)

Gael Moneron (Institut Pasteur)

Jost Enninga (Institut Pasteur)

Monica Sala (DDE Institut Pasteur)

Hervé Waxin (DDE Institut Pasteur)

V. Ponticelli (DDE Institut Pasteur)

Jean-Marc Verbavatz (Université Paris VII)

Sophie Louvet (Université Paris VI)



Practical information


Title of the course

Principles and Applications of Fluorescence Microscopy



Jost Enninga

Unité de Dynamique des interactions hôte-pathogène


David DiGregorio

Unité d'Imagerie dynamique du neurone


Gael Moneron

Unité d'Imagerie dynamique du neurone

Head of studies


Florian Ruckerl

Unité d'Imagerie Dynamique du neurone, Institut Pasteur






2 weeks


February 27 – March 10, 2017

Closing date for application

November 17, 2016


Institut Pasteur Diploma


Attending the course can be validated as "module d'école doctorale" for students registered at partner universities


Credits for a master degree for students registered at Parisian partner universities (Paris VI and Paris VII)



The committee of the course will evaluate applications.


On line registration


Registration fees


Mis à jour le 13/07/2016