This two-weeks theorical and practical course presents RNAs in their diverse forms, from synthesis and maturation to degradation, and multiple roles in gene expression.
Some years ago, the RNA world was divided the protein coding RNAs (mRNAs) and the non-coding RNAs such (tRNAs, snRNAs, snoRNAs and rRNAs). Several important concepts and discoveries such as splicing, catalytic RNA have pointed out the major role of RNA in gene expression. Recently, the discovery of novel classes of RNA like siRNAs, miRNAs and RNA interference (RNAi) shed light on novel major roles of RNA. It revealed a crucial role for RNA in regulating gene expression via transcriptional and post-transcriptional control mechanisms. More recently, deep-sequencing and high-resolution microarrays revealed the existence of a highly complex population of non-coding transcripts. It is now clear that a plethora of stable and unstable RNAs, generated by a pervasive transcription, exists. Many questions remain open about the regulation and function and molecular mechanisms in which these non-coding RNAs are implicated.
Lectures are focused on the multiple roles of RNAs and specific technologies related to RNA studies. These lecture are completed by attending the " RNA Symposium ", during the last day of the course, which takes place at the University Pierre et Marie Curie (Place Jussieu, Paris 5ème).
The aim of the practical course is to quantify nuclear RNA populations in a wild-type strain and a degradation mutant using qRT-PCR and Northern Blot combined to the use of microarrays. A strain deleted for the gene RRP6 encoding a nuclear 3’-5’ exoribonuclease component of the nuclear exosome that is specifically involved in nuclear RNA degradation. The quantification of nuclear RNAs in mutant condition in comparison with wild type condition will be performed. This exploratory methodology could identify nuclear coding and non-coding RNAs and reveal novel specificities for some genes.
The program of the course organized the previous year can be downloaded to get detailed information on the general frame of the course; some topics and practical works may change each year, without altering the general aims and means of the course.
Candidates must have a good knowledge in genetic, molecular biology and biochemistry (level Master 2).
Members of the Course Commitee :
Y. Andéol (Université P. et M. Curie),
G. Badis-Bréard (Institut Pasteur),
C. Branlant (Université Nancy),
E. Coeffier (Institut Pasteur),
M.-C. Daugeron* (Université Paris-Sud 11),
M. Fromont-Racine (Institut Pasteur),
A. Jacquier (Institut Pasteur),
M-C. Maurel (Université P. et M. Curie) ,
C. Parsot (Institut Pasteur),
C. Torchet (Université P. et M. Curie),
A. Zider (Université Paris-Diderot P7).
* Representative of Universities
|Title of the course||Multiple roles of RNAs|
Université Pierre et Marie Curie
Unité de Génétique des interactions macromoléculaires
February 17th to February 28th, 2014
|Closing date for application||September 1, 2013|
Institut Pasteur Diploma
Credit for a master degree for students resgitered at Parisian partner universities