- Institut Pasteur
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A 2-years post-doctoral position is available at Institut Pasteur, in the group « Arboviral virulence factors and toxins », which is part of the Structural Virology unit. The position is financed in the context of a collaborative program between Institut Pasteur and Institut Mérieux.
The aim of the project is to further define the contribution of the dengue virus (DENV) nonstructural protein NS1 to viral pathogenesis. NS1 is a multifunctional protein that localizes to replication factories inside infected cells and is secreted in the extracellular fluid as a soluble hexameric particle (NS1hex). We recently reported that secreted NS1hex is an atypical high density lipoprotein that could possibly interfere with the vascular system during the course of infection (Gutsche et al., PNAS 2011). We also found that DENV NS1hex associates to host proteins during its circulation in the extracellular fluid. We propose to further characterize the nature of these complexes by different approaches (in vitro reconstitution, immunoassays, mass spectrometry, electron microscopy). The role of these complexes will be studied in relevant target cells such as hepatocytes and their prognostic value will be assessed in human patients, in collaboration with the Institut Pasteur International Network.
The applicant should have a PhD with at least 3 years of post-doctoral experience in the field of virology/infectious diseases, and a strong background in biochemistry, molecular and cellular biology. A specific expertise on membrane proteins would be particularly appreciated. Good written and oral communication skills in english are required. The position is expected to start February 1st, 2013 at the latest.
Salary will depend on experience and past achievements.
Applications should include a CV, a summary of previous research activities (2-3 pages) and 2 references (just include name and contact information), to be addressed by e-mail before January 10th, 2013 to
Marie Flamand (firstname.lastname@example.org)