Multiple roles of RNAs

This two-weeks theorical and practical course presents RNAs in their diverse forms, from synthesis and maturation to degradation, and multiple roles they can adopt in various life conditions.

 

Some years ago, the RNA world was divided the protein coding RNAs (mRNAs) and the non-coding RNAs such (tRNAs, snRNAs, snoRNAs and rRNAs). Several important concepts and discoveries such as splicing, catalytic RNA have pointed out the major role of RNA in gene expression. Recently, the discovery of novel classes of RNA like siRNAs, miRNAs and RNA interference (RNAi) shed light on novel major roles of RNA. It revealed a crucial role for RNA in regulating gene expression via transcriptional and post-transcriptional control mechanisms. More recently, deep-sequencing and high-resolution microarrays revealed the existence of a highly complex population of non-coding transcripts. It is now clear that a plethora of stable and unstable RNAs, generated by pervasive transcription, exists. Many questions remain open about the regulation and function and molecular mechanisms in which these non-coding RNAs are implicated.

 

Lectures are focused on the multiple roles of RNAs and specific technologies related to RNA studies. These lectures are completed by attending an international RNA Symposium held the last day of the course at the Université Pierre et Marie Curie (Place Jussieu, Paris 5ème).

 

The aim of the practical course is to learn standard RNA-related technics (Transcriptome analysis using Microarray, Northern Blot and RT-qPCR) through the study of different RNA populations : Total RNA or in parallel RNA purified from nucleus, using a TAP-tagged version of the nuclear cap binding protein CBP20. We are comparing transcripts from a wild-type strain and from a mutant (a strain deleted for the gene RRP6 encoding the nuclear subunit of the exosome). Transcriptome comparisons of nuclear enriched or total RNA from WT and mutant strain are performed, and the analysis of genome-wide results illustrates the diversity of non coding RNAs and transcription.

 

The program of the course organized the previous year can be downloaded to get detailed information on the general frame of the course; some topics and practical works may change each year, without altering the general aims and means of the course.

 

Program of the course "Rôles multiples de l'ARN" (PDF - 500 KB)

 

Candidates must have a good knowledge of genetic, molecular biology and biochemistry (2nd year Mastesr level).

 

Members of the Course Commitee:

 

Y. Andéol (Université P. et M. Curie),

G. Badis-Bréard (Institut Pasteur),

C. Branlant (Université Nancy),

E. Coeffier (Institut Pasteur),

M.-C. Daugeron* (Université Paris-Sud 11),

M. Fromont-Racine (Institut Pasteur),

A. Jacquier (Institut Pasteur),

M-C. Maurel (Université P. et M. Curie) ,

C. Parsot (Institut Pasteur),

C. Torchet (Université P. et M. Curie),

A. Zider (Université Paris-Diderot P7).

 

* Representative of Universities

 

Practical information

 

Title of the course Multiple roles of RNAs
Directors

Yannick Andeol

Pierre et Marie Curie University

 

Gwenaël Badis-Bréard

Macromolecular Interaction Genetics

CNRS, UMR3525

Institut Pasteur

Head of study

Claire Torchet

Université Pierre et Marie Curie

Language English
Participants 20
Duration 2 weeks
Dates

February 23th to March 6th, 2015

Closing date for application September 1, 2014
Diplomas

Institut Pasteur Diploma

 

Credit for a Masters degree for students resgitered at Parisian partner universities

Contact

enseignement@pasteur.fr

Application

On line registration

Registration fees

 

Updated on 17/07/2014

Key numbers

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