The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his wife and can admire his rich and diverse scientific work.
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources needed to speed up and further improve the quality of their outstanding research.
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over the world who come to further their training or top up their degree programs.
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study and traineeship fellowships are available to scientists. Alongside training, dynamism and attractiveness of RIIP will result in the creation of 4-year group for the young researchers.
This two-weeks theorical and practical course presents RNAs in their diverse forms, from synthesis and maturation to degradation, and multiple roles they can adopt in various life conditions.
Some years ago, the RNA world was divided the protein coding RNAs (mRNAs) and the non-coding RNAs such (tRNAs, snRNAs, snoRNAs and rRNAs). Several important concepts and discoveries such as splicing, catalytic RNA have pointed out the major role of RNA in gene expression. Recently, the discovery of novel classes of RNA like siRNAs, miRNAs and RNA interference (RNAi) shed light on novel major roles of RNA. It revealed a crucial role for RNA in regulating gene expression via transcriptional and post-transcriptional control mechanisms. More recently, deep-sequencing and high-resolution microarrays revealed the existence of a highly complex population of non-coding transcripts. It is now clear that a plethora of stable and unstable RNAs, generated by pervasive transcription, exists. Many questions remain open about the regulation and function and molecular mechanisms in which these non-coding RNAs are implicated.
Lectures are focused on the multiple roles of RNAs and specific technologies related to RNA studies. These lectures are completed by attending an international RNA Symposium held the last day of the course at the Université Pierre et Marie Curie (Place Jussieu, Paris 5ème).
The aim of the practical course is to learn standard RNA-related technics (Transcriptome analysis using Microarray, Northern Blot and RT-qPCR) through the study of different RNA populations : Total RNA or in parallel RNA purified from nucleus, using a TAP-tagged version of the nuclear cap binding protein CBP20. We are comparing transcripts from a wild-type strain and from a mutant (a strain deleted for the gene RRP6 encoding the nuclear subunit of the exosome). Transcriptome comparisons of nuclear enriched or total RNA from WT and mutant strain are performed, and the analysis of genome-wide results illustrates the diversity of non coding RNAs and transcription.
The program of the course organized the previous year can be downloaded to get detailed information on the general frame of the course; some topics and practical works may change each year, without altering the general aims and means of the course.