The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his ...
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Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over ...
The mission of the Industrial Partnership team is to detect, promote, assist and protect the inventive activities from research (inventions, know-how and biological materials) conducted at the Institut Pasteur (and in some Institutes of its international network), and transfer there to industrial ...
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study ...
My project concerns the emergence of the mutated strain of CHIKV (E1-A226V) during the outbreak of 2011 in Congo (Brazzaville) in relation with the recent introduction and expansion of Ae. albopictus. As stated in East Africa where this mutation has emerged leading to a better transmission by Ae. albopictus, is this scenario applicable to West Africa?
Genetics, University of Clermont-Ferrand II (Blaise Pascal), France
Medical Entomology Course, Institut Pasteur, Paris, France, 1988
My project is part of the FP7 DenFree project. The tasks where I am involved aim to examine the role of mosquito vectors in dengue emergence, with special emphasis on the European context. I will be implicated in evaluating the ability of European Aedes albopictus to transmit imported DENV strains (originated from DEN endemic regions, Thailand and Cambodia) and in identifying viral genetic changes after experimental alternating cycling to enhance potential of Ae. albopictus from southern France to transmit imported DEN strains from Southeast Asia.
Ecole Technique Supérieure du Laboratoire, Paris, France
My project is part of the FP7 Vectorie project on surveillance of two arthropod-borne diseases, West-Nile and Chikungunya, in Europe. Our laboratory is involved in defining the vector competence of Ae. albopictus mosquitoes from different European countries for CHIK virus at low temperatures. I am responsible of three main tasks which are: (i) experimentally challenges of field-collected European populations of Ae. albopictus (France, Italy, Montenegro) with different CHIKV isolates; (ii) evaluation of mosquito thermo-preference and (iii) effects of temperature on gene expression in temperate Ae. albopictus infected by different strains of CHIKV and submitted to thermal stress, using a transcriptomic approach provided by the technology of high throughput RNA sequencing.
D.E.A. (Master), 2006
Microbial Ecology, University of Lyon I (Claude Bernard), France
Microbial Ecology, University of Lyon I (Claude Bernard), France
My project is part of the EMIDA project and concerns the risk for European mosquitoes to become a vector of the Rift Valley fever virus. To evaluate this risk, we have to investigate (i) the molecular identification of RVFV vectors (Ae. vexans complex and Cx. pipiens complex) with special focus on European counterparts of tropical mosquitoes species through the use of molecular tools and estimate the abundance and dynamics of potential RVFV vectors in Camargue (France); (ii) characterize the competence of potential European RVFV vectors in comparison with their African counterparts, and (iii) investigate through in vivo experiments if RVFV can be adapted to European mosquitoes.
Infectiologie cellulaire et moléculaire , vaccinologie, Université François Rabelais, Tours, France
Science de la vie et de la santé, Université François Rabelais, Tours, France
My PhD project aims to analyze the risk for CHIKV to emerge in Latin America, a continent where the typical CHIKV vectors Ae. aegypti and Ae. albopictus are present in high densities, and where several CHIKV importation cases have been reported. Until now, local CHIKV transmission has never been reported in Latin America. To assess this risk, I will: (i) evaluate the vector competence of different field-collected Aedes albopictus from Latin America to transmit several genotypes of CHIKV, (ii) study CHIKV evolution and adaptation to Latin American vectors using in vitro and in vivo systems and, (iii) trace the evolutionary history of Aedes albopictus using phylogenetic approaches.
D.E.A. (Master), 2011
Genetic and biodiversity managment, University Pierre and Marie Curie / Institut Pasteur, Paris, France
Ph.D. Student, 2012-2015
University Pierre and Marie Curie / Institut Pasteur, Paris, France.
My research is a part of the European Union’s DENFREE project. With the recent autochthonous cases of dengue in France (2010), Croatia (2010) and in the Portuguese island of Madeira (2012), dengue has arrived in Europe. As an arbovirus, dengue virus (DENV) requires replication in both a vertebrate host and insect vector, and interactions between these two disparate hosts within a permissive environment determine the outcome of transmission. My main objectives are: (i) to select a DENV more adapted to be transmitted by European Aedes albopictus using serial passages, and (ii) to generate molecular clones of these adapted strains, allowing me to characterize the molecular mechanisms behind this adaptation. These studies will provide unique insights into viral-vector interactions and their impact on fitness in mammalian systems.
Microbiology, University of Colorado Anschutz Medical Campus, Aurora Colorado, USA
Bachelor of Science, 2005
Biology, Colorado Mesa University, Grand Junction, Colorado, USA
My project aims to analyze the tripartite interactions between insecticide resistance genes, Wolbachia and arboviruses in mosquito vectors. The use of insecticides plays a key role in the prevention and control of vector-borne diseases. However, its efficacy has been challenged by the emergence of insecticide resistance. Mutations conferring insecticide resistance are often associated with a measurable fitness cost, suggesting the existence of resource-based trade-offs that could affect the replication of Wolbachia and pathogens. The overall goal of my project is to assess whether insecticide resistance genes can affect the transmission of Rift Valley Fever Virus (RVFV) and West Nile Virus (WNV) in the Culexpipiens mosquito. This goal will be achieved by pursuing four specific aims: (i) comparing vector competence of resistant and susceptible mosquitoes for RVFV and WNV, (ii) examining the effect of Wolbachia genetic groups and Cx. pipiens subspecies in the interactions between insecticide-resistance genes and RVFV and WNV, (iii) assessing the effect of temperature in these tripartite interactions, and (iv) exploring underlying genetic molecular mechanisms involved in the observed phenotypes. This project will provide a unique opportunity to know whether insecticide resistance genes may change the transmission of arboviruses by affecting the vector competence.
Parasitology: University Montpellier 2, France
Evolution, Ecology and Genetics: University Montpellier 2, France
Postdoctoral Fellow, 2012-2014
University of La Réunion/CRVOI: La Réunion Island, France
AtyameC., Julien Cattel, Cyrille Lebon, Olivier Flores et al. Wolbachia-based mosquitoes proves efficient under semi-field conditions population control strategy targeting Culexquinquefasciatus. (Inrevision in PlosOne).
Atyame C., Pierrick Labbé, François Rousset, Marwa Beji et al. Stable coexistence of incompatible Wolbachia along a narrow contact zone in mosquito field populations. (In revision in Molecular Ecology).
Atyame C., Labbé P., Dumas E., Milesi P., Charlat S. et al. (2014). Wolbachia divergence and the evolution of cytoplasmic incompatibility in Culex pipiens. Plos One, 9 (1): e87336.
Pocquet N., Milesi P., Makoundou P., Unal S., Zumbo B., Atyame C. et al. (2013). Multiple insecticide resistances in the disease vector Culex p. quinquefasciatus from Western Indian Ocean. Plos One, 8 (10): e77855.
Dumas E., Atyame C., Milesi P., Fonseca D. et al. (2013). Population structure of Wolbachia and cytoplasmic introgression in a complex of mosquito species. BMC Evolutionary Biology, 13 (1): 181).
Duron O., Bernard J., Atyame C., Dumas E. & Weill M. (2012). Rapid evolution of Wolbachia incompatibility types. Proceedings of the Royal Society B-Biological Sciences, 279 (1746): 4473-80.
Atyame C., Delsuc F., Pasteur N., Weill M. & Duron O. (2011). Diversification of Wolbachia endosymbiont in the Culex pipiens mosquito. Molecular Biology and Evolution, 28 (10): 2761-2772.
Atyame C., Duron O., Tortosa P., Pasteur N., Fort P., & Weill M. (2011). Multiple Wolbachia determinants control the evolution of cytoplasmic incompatibilities in Culex pipiens mosquito populations. Molecular Ecology, 20 (2): 286-298.
Atyame C., Pasteur N., Dumas E., Tortosa P., Tantely M. et al. (2011). Cytoplasmic incompatibility as a means to control Culex pipiens quinquefasciatus mosquito in the islands of the southwestern Indian Ocean. PLoS Neglected Tropical Diseases, 5 (12): e1440.
Atyame C., Sommerer N., Rofidal V., Hirtz C., Rossignol M. et al. (2010). Excreted/secreted proteins from Trypanosome procyclic strains. Journal of Biomedicine and Biotechnology, 212817.
My PhD project aims to analyze the antiviral ability of transgenic mosquitoes. We are currently generating several miRNA-based transgenic Aedes aegypti and Aedes albopictus designed to suppress the DENV and CHIKV replication in mosquitoes. To evaluate the possibility of applying this strategy into practical disease control, we will: (i) experimentally challenge the transgenic mosquitoes under different conditions, e.g. virus titers, incubation temperatures, and humidity to analyze the suppression efficiency on viral replication; (ii) test male mating competitiveness to acquire the fitness impact information among transgenic populations; (iii) develop a novel transgenic control strategy for arboviruses.
Master of Science, 2011
Department of Entomology, National Chung Hsing University, Taichung, Taiwan
PhD student, 2014-2017
Pasteur-Paris University International doctoral program, Institut Pasteur, Paris, France - University Pierre and Marie Curie