Lab members





Current Position

Principal Investigator

Chef de Laboratoire

Arboviruses and Insect Vectors



D.E.A. (Master), 1988

Ecology, University of Toulouse III (Paul Sabatier), France


Medical Entomology Course, Institut Pasteur, Paris, France, 1988


Ph.D., 1994

Ecophysiology and Population dynamics of terrestral invertebrates, University of Paris XI, France (Nicole Pasteur)


Postdoctoral Fellow, 1994-1996

Institut Pasteur, Ecology of Vectorial Systems (François Rodhain)


H.D.R., 2000

University of Paris XII, France



marie-vazeille.jpgMARIE VAZEILLE, PhD




Current Position

My project concerns the emergence of the mutated strain of CHIKV (E1-A226V) during the outbreak of 2011 in Congo (Brazzaville) in relation with the recent introduction and expansion of Ae. albopictus. As stated in East Africa where this mutation has emerged leading to a better transmission by Ae. albopictus, is this scenario applicable to West Africa?


Ph.D., 1987

Genetics, University of Clermont-Ferrand II (Blaise Pascal), France


Medical Entomology Course, Institut Pasteur, Paris, France, 1988









Current Position

My project is part of the FP7 DenFree project. The tasks where I am involved aim to examine the role of mosquito vectors in dengue emergence, with special emphasis on the European context. I will be implicated in evaluating the ability of European Aedes albopictus to transmit imported DENV strains (originated from DEN endemic regions, Thailand and Cambodia) and in identifying viral genetic changes after experimental alternating cycling to enhance potential of Ae. albopictus from southern France to transmit imported DEN strains from Southeast Asia.




BTS, 1985

Ecole Technique Supérieure du Laboratoire, Paris, France






faustine_louis.jpgFAUSTINE LOUIS, PhD




Current Position

My project is part of the EMIDA project and concerns the risk for European mosquitoes to become a vector of the Rift Valley fever virus. To evaluate this risk, we have to investigate (i) the molecular identification of RVFV vectors (Ae. vexans complex and Cx. pipiens complex) with special focus on European counterparts of tropical mosquitoes species through the use of molecular tools and estimate the abundance and dynamics of potential RVFV vectors in Camargue (France); (ii) characterize the competence of potential European RVFV vectors in comparison with their African counterparts, and (iii) investigate through in vivo experiments if RVFV can be adapted to European mosquitoes.



Master, 2008

Infectiologie cellulaire et moléculaire , vaccinologie, Université François Rabelais, Tours, France

Ph.D., 2013

Science de la vie et de la santé, Université François Rabelais, Tours, France



Louis F, Bézier A, Periquet G, Ferras C, Drezen JM, Dupuy C, 2013. The bracovirus genome of the parasitoid wasp Cotesia congregata is amplified within 13 replication units, including sequences not packaged in the particles. J Virol 87(17) : 9649-60.

Bézier A, Louis F, Jancek S, Periquet G, Thézé J, Gyapay G, Musset K, Lesobre J, Lenoble P, Dupuy C, Gundersen-Rindal D, Herniou EA, Drezen JM, 2013. Functional endogenous viral elements in the genome of the parasitoid wasp Cotesia congregata: insights into the evolutionary dynamics of bracoviruses. Philos Trans R Soc Lond B Biol Sci 368 (1626) : 20130047.

Dupuy C, Periquet G, Serbielle C, Bézier A, Louis F, Drezen JM, 2011. Transfer of a chromosomal Maverick to endogenous bracovirus in a parasitoid wasp. Genetica 139(4):489-496.







Agent de Laboratoire


Current Position

I am in charge of the maintenance of the insectaries.











Current Position


My PhD project aims to analyze the risk for CHIKV to emerge in Latin America, a continent where the typical CHIKV vectors Ae. aegypti and Ae. albopictus are present in high densities, and where several CHIKV importation cases have been reported. Until now, local CHIKV transmission has never been reported in Latin America. To assess this risk, I will: (i) evaluate the vector competence of different field-collected Aedes albopictus from Latin America to transmit several genotypes of CHIKV, (ii) study CHIKV evolution and adaptation to Latin American vectors using in vitro and in vivo systems and, (iii) trace the evolutionary history of Aedes albopictus using phylogenetic approaches.



D.E.A. (Master), 2011

Genetic and biodiversity managment, University Pierre and Marie Curie / Institut Pasteur, Paris, France


Ph.D. Student, 2012-2015

University Pierre and Marie Curie / Institut Pasteur, Paris, France.







Post-doctoral fellow


Current Position

My research is a part of the European Union’s DENFREE project. With the recent autochthonous cases of dengue in France (2010), Croatia (2010) and in the Portuguese island of Madeira (2012), dengue has arrived in Europe. As an arbovirus, dengue virus (DENV) requires replication in both a vertebrate host and insect vector, and interactions between these two disparate hosts within a permissive environment determine the outcome of transmission. My main objectives are: (i) to select a DENV more adapted to be transmitted by European Aedes albopictus using serial passages, and (ii) to generate molecular clones of these adapted strains, allowing me to characterize the molecular mechanisms behind this adaptation. These studies will provide unique insights into viral-vector interactions and their impact on fitness in mammalian systems.





Ph.D., 2013

Microbiology, University of Colorado Anschutz Medical Campus, Aurora Colorado, USA


Bachelor of Science, 2005

Biology, Colorado Mesa University, Grand Junction, Colorado, USA





Post-doctoral fellow


Current Position

My project aims to analyze the tripartite interactions between insecticide resistance genes, Wolbachia and arboviruses in mosquito vectors. The use of insecticides plays a key role in the prevention and control of vector-borne diseases. However, its efficacy has been challenged by the emergence of insecticide resistance. Mutations conferring insecticide resistance are often associated with a measurable fitness cost, suggesting the existence of resource-based trade-offs that could affect the replication of Wolbachia and pathogens. The overall goal of my project is to assess whether insecticide resistance genes can affect the transmission of Rift Valley Fever Virus (RVFV) and West Nile Virus (WNV) in the Culex pipiens mosquito. This goal will be achieved by pursuing four specific aims: (i) comparing vector competence of resistant and susceptible mosquitoes for RVFV and WNV, (ii) examining the effect of Wolbachia genetic groups and Cx. pipiens subspecies in the interactions between insecticide-resistance genes and RVFV and WNV, (iii) assessing the effect of temperature in these tripartite interactions, and (iv) exploring underlying genetic molecular mechanisms involved in the observed phenotypes. This project will provide a unique opportunity to know whether insecticide resistance genes may change the transmission of arboviruses by affecting the vector competence. 



Master, 2007

Parasitology: University Montpellier 2, France

Ph.D, 2011

Evolution, Ecology and Genetics: University Montpellier 2, France

Postdoctoral Fellow, 2012-2014

University of La Réunion/CRVOI: La Réunion Island, France     



Atyame C., Julien Cattel, Cyrille Lebon, Olivier Flores et al. Wolbachia-based mosquitoes proves efficient under semi-field conditions population control strategy targeting Culex quinquefasciatus. (In revision in Plos One).

Atyame C., Pierrick Labbé, François Rousset, Marwa Beji et al. Stable coexistence of incompatible Wolbachia along a narrow contact zone in mosquito field populations. (In revision in Molecular Ecology).

Atyame C., Labbé P., Dumas E., Milesi P., Charlat S. et al. (2014). Wolbachia divergence and the evolution of cytoplasmic incompatibility in Culex pipiens. Plos One, 9 (1): e87336.

Pocquet N., Milesi P., Makoundou P., Unal S., Zumbo B., Atyame C. et al. (2013). Multiple insecticide resistances in the disease vector Culex p. quinquefasciatus from Western Indian Ocean. Plos One, 8 (10): e77855.

Dumas E., Atyame C., Milesi P., Fonseca D. et al. (2013). Population structure of Wolbachia and cytoplasmic introgression in a complex of mosquito species. BMC Evolutionary Biology, 13 (1): 181).

Duron O., Bernard J., Atyame C., Dumas E. & Weill M. (2012). Rapid evolution of Wolbachia incompatibility types. Proceedings of the Royal Society B-Biological Sciences, 279 (1746): 4473-80.

Atyame C., Delsuc F., Pasteur N., Weill M. & Duron O. (2011). Diversification of Wolbachia endosymbiont in the Culex pipiens mosquito. Molecular Biology and Evolution, 28 (10): 2761-2772.

Atyame C., Duron O., Tortosa P.,  Pasteur N., Fort P., & Weill M. (2011). Multiple Wolbachia determinants control the evolution of cytoplasmic incompatibilities in Culex pipiens mosquito populations. Molecular Ecology, 20 (2): 286-298.  

Atyame C., Pasteur N., Dumas E., Tortosa P., Tantely M. et al. (2011). Cytoplasmic incompatibility as a means to control Culex pipiens quinquefasciatus mosquito in the islands of the southwestern Indian Ocean. PLoS Neglected Tropical Diseases, 5 (12): e1440.

Atyame C., Sommerer N., Rofidal V., Hirtz C., Rossignol M. et al. (2010). Excreted/secreted proteins from Trypanosome procyclic strains. Journal of Biomedicine and Biotechnology, 212817.


image001.jpgPei-Shi YEN


PhD student


Current Position

My PhD project aims to analyze the antiviral ability of transgenic mosquitoes. We are currently generating several miRNA-based transgenic Aedes aegypti and Aedes albopictus designed to suppress the DENV and CHIKV replication in mosquitoes. To evaluate the possibility of applying this strategy into practical disease control, we will: (i) experimentally challenge the transgenic mosquitoes under different conditions, e.g. virus titers, incubation temperatures, and humidity to analyze the suppression efficiency on viral replication; (ii) test male mating competitiveness to acquire the fitness impact information among transgenic populations; (iii) develop a novel transgenic control strategy for arboviruses.



Master of Science, 2011

Department of Entomology, National Chung Hsing University, Taichung, Taiwan


PhD student, 2014-2017

Pasteur-Paris University International doctoral program, Institut Pasteur, Paris, France - University Pierre and Marie Curie








Master student






Visiting scientist






camilo.jpgCAMILO ARIAS-GOETA, PhD student


PhD student








Post-doctoral fellow






karima-zouache-2.jpgKARIMA ZOUACHE, PhD


Post-doctoral fellow


Updated on 03/03/2015