The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his ...
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources ...
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over ...
The mission of the Industrial Partnership team is to detect, promote, assist and protect the inventive activities from research (inventions, know-how and biological materials) conducted at the Institut Pasteur (and in some Institutes of its international network), and transfer there to industrial ...
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study ...
Yau WL, Pescher P, Macdonald A, Hem S, Zander D, Retzlaff S, Blisnick T, Rotureau B, Rosenqvist H, Wiese M, Bastin P, Clos J, Späth GF. The Leishmania donovani chaperone cyclophilin 40 is essential for intracellular infection independent of its stage-specific phosphorylation status. Mol Microbiol. 2014, in press.
Dacher M, Morales MA, Pescher P, Leclercq O, Rachidi N, Prina E, Cayla M, Descoteaux A, Späth GF. Probing druggability and biological function of essential proteins in Leishmania combining facilitated null mutant and plasmid shuffle analyses. Mol Microbiol. 2014 in press.
Rachidi N, Taly JF, Durieu E, Leclercq O, Aulner N, Prina E, Pescher P, Notredame C, Meijer L, Späth GF. Pharmacological assessment defines the Leishmania donovani casein kinase 1 as a drug target and reveals important functions in parasite viability and intracellular infection. Antimicrob Agents Chemother. 2014 Mar;58(3):1501-15
Müller AJ, Aeschlimann S, Olekhnovitch R, Dacher M, Späth GF, Bousso P. Photoconvertible Pathogen Labeling Reveals Nitric Oxide Control of Leishmania major Infection In Vivo via Dampening of Parasite Metabolism. Cell Host Microbe. 2013 Oct 16;14(4):460-7.
Tsigankov P, Gherardini PF, Helmer-Citterich M, Späth GF, Zilberstein D, “Phosphoproteomic Analysis of Differentiating Leishmania Parasites Reveals a Unique Stage-Specific Phosphorylation Motif”, J Proteome Res. 2013 Jul 5;12(7):3405-12.
Leclercq O, Bartho K, Duelsner E, von Kleist L, Gherardini PF, Palmeri A, Helmer-Citterich M, Baumgart S, Späth GF, “Enrichment of Leishmania donovani ATP-binding proteins using a staurosporine capture compound”, J Proteomics. 2013 Jun 28;86:97-104.
Aulner N, Danckaert A, Rouault-Hardoin E, Desrivot J, Helynck O, Commere PH, Munier-Lehmann H, Späth GF, Shorte SL, Milon G, Prina E, “High Content Analysis of Primary Macrophages Hosting Proliferating Leishmania Amastigotes: Application to Anti-leishmanial Drug Discovery”, PLoS Negl Trop Dis. 2013 Apr 4;7(4):e2154. Print 2013 Apr.
Foucher AL, Rachidi N, Gharbi S, Blisnick T, Bastin P, Pemberton IK, Späth GF, "Apoptotic marker expression in the absence of cell death in staurosporine treated Leishmania donovani", Antimicrob Agents Chemother. 2013 Mar;57(3):1252-61.
Horjales S, Schmidt-Arras D, Limardo RR, Leclercq O, Obal G, Prina E, Turjanski AG, Späth GF, Buschiazzo A. Structure, “The crystal structure of the MAP kinase LmaMPK10 from Leishmania major reveals parasite-specific features and regulatory mechanisms”, 2012 Oct 10;20(10):1649-60. doi: 10.1016/j.str.2012.07.005. Epub 2012 Aug 9.
Müller AJ, Filipe-Santos O, Eberl G, Aebischer T, Späth GF, Bousso P. Immunity, “CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation”, 2012 Jul 27;37(1):147-57. doi: 10.1016/j.immuni.2012.05.015. Epub 2012 Jun 21.
Giraud E, Lecoeur H, Soubigou G, Coppee JY, Milon G, Prina E, Lang T. Distinct transcriptional signatures of bone marrow-derived C57BL/6 and DBA/2 dendritic leucocytes hosting live Leishmania amazonensis amastigotes. PLoS Negl Trop Dis, 2012, 6: e1980.
Dash PK, Boutonnier A, Prina E, Sharma S, Reiter P. Development of a SYBR green I based RT-PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti. Virol J, 2012, 9: 27.
Palmeri A, Gherardini PF, Tsigankov P, Ausiello G, Späth GF, Zilberstein D, Helmer-Citterich M., “PhosTryp: a phosphorylation site predictor specific for parasitic protozoa of the family trypanosomatidae”, BMC Genomics. 2011 Dec 19;12:614.
Mani J, Güttinger A, Schimanski B, Heller M, Acosta-Serrano A, Pescher P, Späth G, Roditi I, "Alba-domain proteins of Trypanosoma brucei are cytoplasmic RNA-binding proteins that interact with the translation machinery", PLoS One. 2011;6(7):e22463. Epub 2011 Jul 21.
Pescher P, Blisnick T, Bastin P, Späth GF. Quantitative proteome profiling informs on phenotypic traits that adapt Leishmania donovani for axenic and intracellular proliferation. Cell Microbiol. 2011 Jul;13(7):978-91
Schmidt-Arras D, Leclercq O, Gherardini PF, Helmer-Citterich M, Faigle W, Loew D, Späth GF. Adaptation of a 2D in-gel kinase assay to trace phosphotransferase activities in the human pathogen Leishmania donovani. J Proteomics. 2011 Aug 24;74(9):1644-51
Hem S, Gherardini PF, Osorio y Fortéa J, Hourdel V, Morales MA, Watanabe R, Pescher P, Kuzyk MA, Smith D, Borchers CH, Zilberstein D, Helmer-Citterich M, Namane A, Späth GF. Identification of Leishmania-specific protein phosphorylation sites by LC-ESI-MS/MS and comparative genomics analyses. Proteomics. 2010;10(21):3868-83.
Yau WL, Blisnick T, Taly JF, Helmer-Citterich M, Schiene-Fischer C, Leclercq O, Li J, Schmidt-Arras D, Morales MA, Notredame C, Romo D, Bastin P, Späth GF. Cyclosporin A treatment of Leishmania donovani reveals stage-specific functions of cyclophilins in parasite proliferation and viability. PLoS Negl Trop Dis. 2010 Jun 29;4(6):e729.
Foucher AL, Späth GF, Pemberton IK. Probing the dynamic nature of signalling pathways by IMAC and SELDI-tof MS. Arch Physiol Biochem. 2010 Oct-Dec;116(4-5):163-73.
Dujardin JC, González-Pacanowska D, Croft SL, Olesen OF, Späth GF. Collaborative actions in anti-trypanosomatid chemotherapy with partners from disease endemic areas. Trends Parasitol. 2010 Aug;26(8):395-403. Review.
Morales MA, Watanabe R, Dacher M, Chafey P, Osorio y Fortéa J, Scott DA, Beverley SM, Ommen G, Clos J, Hem S, Lenormand P, Rousselle JC, Namane A, Späth GF. Phosphoproteome dynamics reveal heat-shock protein complexes specific to the Leishmania donovani infectious stage. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8381-6.
Morales MA, Pescher P, Späth GF. Leishmania major MPK7 protein kinase activity inhibits intracellular growth of the pathogenic amastigote stage. Eukaryot Cell. 2010 Jan;9(1):22-30.
Filipe-Santos O, Pescher P, Breart B, Lippuner C, Aebischer T, Glaichenhaus N, Späth GF, Bousso P. A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection. Cell Host Microbe. 2009 Jul 23;6(1):23-33.
Späth GF, Schlesinger P, Schreiber R, Beverley SM. A novel role for Stat1 in phagosome acidification and natural host resistance to intracellular infection by Leishmania major. PLoS Pathog. 2009 5(4):e1000381.
Peduto L, Dulauroy S, Lochner M, Späth GF, Morales MA, Cumano A, Eberl G. Inflammation recapitulates the ontogeny of lymphoid stromal cells. J Immunol. 2009 182(9):5789-99.
Rotureau B, Morales MA, Bastin P, Späth GF. The flagellum-mitogen-activated protein kinase connection in Trypanosomatids: a key sensory role in parasite signalling and development? Cell Microbiol. 2009 11(5):710-8. doi: 10.1111/j.1462-5822.2009.01295.x. Epub 2009 Feb 4.
Späth G.F., McDowell M.A., and S.M. Beverley SM, “Leishmania major intracellular survival is not altered in SHP-1 deficient me(v) or CD45(-/-) mice”, Exp Parasitol. 2008 120(3):275-9
Morales M.A., Watanabe R., Laurent C., Lenormand P., Rousselle J.C., Namane A. and G.F. Späth, “Phosphoproteomic analysis of Leishmania donovani pro- and amastigote stages”, Proteomics, 2008 18:350-363.
Frevert U., Späth G.F. and H. Yee, “Exoerythrocytic development of Plasmodium gallinaceum in the White Leghorn chicken”. Int J Parasitology, 2008, 38:655-72.
Morales M.A., Renaud O., Faigle W., Shorte S.L. and G. F. Späth, “ Over-expression of Leishmania major MAP kinases reveals stage-specific induction of phosphotransferase activity”, Int. J. of Parasitology, 2007, 11:1187-99.
Boulanger, N., C. Lowenberger, P. Volf, R. Ursic, L. Sigutova, L. Sabatier, M. Svobodova, R. Brun, G. Späth, S.M. Beverley, S. Perrotey, B. Pesson, and P. Bulet (2004), “Characterization of a defensin from the sand fly Phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite Leishmania major” , Infection & Immunity, 2004 72: 7140.
Soos T.J., Marsland, B.J. Späth G.F, Kopf M., and D. Littman, “Impaired Th2 cell differentiation in the absence of PKCtheta”, J. Exp. Med. 2004 200:181.
Amprey J.L., Im J.S., Turco S.J., Murray H.W., Illarionov P.A., Besra G.S., Porcelli S.A., and G. F. Späth, “Innate recognition of Leishmania donovani glycolipids by CD1d-restricted T cells induces an early protective immune response during liver infection”, J. Exp. Med 2004, 200:895.
Amprey J., Späth G.F., and S. Porcelli, “Inhibition of CD1 expression in human dendritic cells during intracellular infection with Leishmania donovani”, Infection & Immunity, 2004 72:589.
Uzonna J., Späth G.F., Beverley S.M., and P. Scott, “ Vaccination with phosphoglycan-deficient lpg2- knock-out Leishmania major protects highly susceptible mice from virulent challenge”, J. Immunol., 2004 172:3793.
Späth G.F., Lye L.F. and S.M. Beverley, “Identification of a compensatory mutant (lpg2-REV) in Leishmania major able to survive as amastigotes within macrophages without LPG2-dependent glycoconjugates, and its significance to virulence and immunization strategies”, Infection & Immunity, 2004 72:3622.
Späth G.F., Lye, L.F., Segawa, H., Sacks, D., Turco S.J.and S.M. Beverley, " Persistence without Pathology in Phosphoglycan-Deficient Leishmania major”, Science, 2003 29:124.
Späth G.F., Garraway, L., Turco S.J.and S.M. Beverley, “The role(s) of lipophosphoglycan (LPG) in the establishment of Leishmania major infections in mammalian hosts”, Proc. Natl. Acad. Sci. U S A, 2003 100:9536.
Salvatore J. Turco, Gerald Späth, and Stephen M. Beverley, " Is Lipophosphoglycan a Virulence Factor? A surprising diversity between Leishmania species", Trends in Parasitology, 2001 17:223-6.
Späth G.F., and S.M.Beverley, "An LPG-independent approach for isolation of infective Leishmania metacyclic promastigotes by density gradient centrifugation", Exp. Parasitology, 2001 19:97-103.
Sacks D.L., Modi G., Rowton E., Späth G., Turco S.J. and S.M. Beverley, "The role of phosphoglycans in Leishmania-Sand Fly interactions", Proc. Natl. Acad. Sci. 2000 97:406-411.
Späth G., Epstein L., Leader B., Singer S., Avila H., Turco S.J. and S.M.Beverley, "LPG is a virulence factor distinct from related glycoconjugates in the protozoan parasite Leishmania major", Proc. Natl. Acad. Sci., 2000 97:9258.
Späth G. and Weiss M.C., "Hepatocyte Nuclear Factor 4 provokes expression of epithelial marker genes, acting as a morphogen in dedifferentiated hepatoma cells, J. Cell Biol. 1998 4:935-946.
Bally A., Späth G., Bender V. and Weiss M.C., "Phenotypic effects of the forced expression of HNF4 and HNF1 are conditioned by properties of the recipient cell", J. Cell Sci. 1998 111:2411-2421.
Amicone L., Spagnoli F.M., Späth G., Giordano S., Tommasini C., Bernardini S., DeLuca V., Della Rocca C., Weiss, M.C., Comoglio P.M. and Tripodi M., "Immortalization of differentiated hepatocytes by transgenic expression of truncated Met", EMBO 1997 16:495-503.
Späth G. and Weiss M.C., "Hepatocyte Nuclear Factor 4 expression overcomes repression of the hepatic phenotype in dedifferentiated hepatoma cells", Mol. Cell Biol. 1997 17:1913-1922.
Späth G., Ramadori G., Rittner C. and Schneider P., "Expression of the C8 genes during cytokine-mediated in vitro induction of the acute phase response", Exp. Clin. Immunogenet. 1995 12:53-60.
Protein Phosphorylation in Parasites: Novel Targets for Antiparasitic Intervention. Christian Doerig (Editor), Gerald Späth (Editor), Martin Wiese (Editor), Paul M. Selzer (Series Editor). ISBN: 978-3-527-33235-9, 456 pages, December 2013, Wiley-Blackwell.