The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his wife and can admire his rich and diverse scientific work.
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources needed to speed up and further improve the quality of their outstanding research.
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over the world who come to further their training or top up their degree programs.
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study and traineeship fellowships are available to scientists. Alongside training, dynamism and attractiveness of RIIP will result in the creation of 4-year group for the young researchers.
Capture of Single Antigen-Specific B cells to Produce Human Monoclonal Antibodies to Pathogens
To achieve our research goals, we are making use of a very efficient method to clone and express immunoglobulins from human single B cells specific to viral antigens.
Briefly, peripheral B cells are isolated from PBMCs of infected individuals by Ficoll separation and magnetic B-cell enrichment. Single antigen-specific B cells are identified by staining with the biotinylated antigen, and fluorescently labeled antibodies to B-cell surface markers. Single B cells are then sorted into 96-well PCR plates using a single-cell FACS sorter.
Single-cell cDNA synthesis is performed, and followed by two successive rounds of PCR amplifications of the cDNA fragments encoding the immunoglobulin heavy-chain (IgH) and light chains (Igk and Igl). All PCR products are sequenced to perform detailed Ig gene sequence analyses.
Restriction sites are introduced by the nested primers during the second PCR, and are used to clone the IgH, Igk and Igl DNA fragments into human Ig-expressing vectors.
Monoclonal antibodies are produced by co-transfection of HEK-293T cells with IgH and IgL expression-vectors using the polyethylenimine-precipitation method.
The antibodies are affinity purified from culture supernatants, and are finally characterized in details at a molecular and functional level.