Dendritic Cell Immunobiology (Matthew Albert)


Fabrice Lemaître, Charlotte Auriau, Alexander Bachmann, Philippe Bousso, Caroline Demangel, Lucie Peduto, George N. Thalmann & Matthew L. Albert (2012). Pre-existing BCG specific immunity improves intravesical immunotherapy for bladder cancer. Science Translational Medicine, 6:137ra72.


Joubert PE, Werneke SW, de la Calle C, Guivel-Benhassine F, Giodini A, Peduto L, Levine B, Schwartz O, Lenschow DJ, Albert ML (2012). Chikungunya virus-induced autophagy delays caspase-dependent cell death. J Exp Med. 2012 May 7;209(5):1029-47.


Yatim N and Albert ML (2011). Dying to Replicate: the orchestration of the viral life cycle, cell death pathways and immunity. Immunity. Immunity. Oct 28;35(4):478-90.


Casrouge A, Decalf J, Ahloulay M, Lababidi C, Mansour H, Vallet-Pichard A, Mallet V, Mottez E, Mapes J, Fontanet A, Pol S, Albert ML (2011). Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV. Journal of Clinical Investigation. Jan 4;121(1):308-17.


Cécile Alanio, Fabrice Lemaitre, Helen KW Law, Milena Hasan, and Matthew L. Albert (2010). Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies. Blood. 115(18):3718-25.


Dynamics of Immune Responses  (Philippe Bousso)


Moreau H, Lemaître F, Terriac E, Azar G, Piel M, Lennon-Dumenil A, Bousso P. 2012. Dynamic In Situ Cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo. Immunity, 37:351-363.


Müller AJ, Filipe-Santos O, Eberl G, Aebischer T, Späth GF, Bousso P. 2012. CD4 T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity, 37:147-157.


Celli S., Albert M.L and P. Bousso. 2011. Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy. Nat. Med. 17:744-50


Deguine, J., Breart, B. , Lemaître, F., Di Santo, J.D. and P. Bousso. 2010. Intravital imaging reveals distinct dynamics for NK cells and CD8+ T cells during tumor regression. Immunity 33:632


Beuneu, H., Lemaître, F., Deguine, J. Moreau, H.M., Bouvier, I., Garcia, Z. and P. Bousso. 2010. Visualizing the functional diversification of CD8+ T cell responses in lymph nodes. Immunity 33:412


Publications of Lymphopoiesis unit (Ana Cumano)


Cumano, A. & Godin, I.

Ontogeny of the hematopoietic system.

Annu. Rev. Immunol. 25 : 745-785 (2007)


Possot, C., Schmutz, S., Chea, S., Boucontet, L., Louise, A., Cumano, A. & Golub, R.

Notch signaling is necessary for adult, but not fetal, development of RORgt+ innate lymphoid cells.

Nature Immunol. 12 : 949-959 (2011)


Pereira, P., Boucontet, L. & Cumano, A.

Temporal predisposition to ab and gd T cell fates in the thymus.

J. Immunol. 188 : 1600-1608 (2012)


Kieusseian, A., Brunet de la Grange, P., Burlen-Defranoux, O., Godin I. & Cumano, A.

Immature hematopoietic stem cells undergo maturation in the fetal liver.

Development 139 : 3521-3530 (2012)


Pereira de Sousa, A., Berthault, C., Granato, A., Dias, S., Ramond, C., Kee, B.L., Cumano, A. & Vieira P.

Inhibitors of DNA binding proteins restrict T cell potential by repressing Notch1 expression in Flt3-negative common lymphoid progenitors.

J. Immunol. 189 : 3822-3830 (2012)


Immobiology of Infection (Caroline Demangel)


Guenin-Macé L, Veyron-Churlet R, Thoulouze MI, Romet-Lemonne G, Hong H, Leadlay PF, Danckaert A, Ruf MT, Mostowy S, Zurzolo C, Bousso P, Chrétien F, Carlier MF , Demangel C (2013) Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation, J Clin Invest, in press.


Guenin-Macé L, Carrette F, Asperti-Boursin F, Le Bon A, Caleechurn L, Di Bartolo V, Fontanet A, Bismuth G, Demangel C. (2011) Mycolactone impairs T cell homing by suppressing microRNA control of L-selectin expression. Proc Natl Acad Sc USA, 108(31):12833-12838.


Boulkroun, S., Guenin-Macé, L., Monot, M., Caleechurn, L., Merckx, A., Langsley, G., Bismuth, G., Di Bartolo, V. and Demangel, C. (2010) Mycolactone suppresses T cell responsiveness by altering both early signalling and post-translational events. J Immunol, 184:1436-1444.


Demangel, C., Stinear, T.P. and Cole, S.T. (2009) BU: reductive evolution enhances pathogenicity of Mycobacterium ulcerans. Nat Rev Microbiol, 7:50-60.


Coutanceau, E., Decalf, J., Babon, A., Winter, N., Cole, S.T., Albert, M.L. and Demangel, C. (2007) Selective suppression of dendritic cell functions by Mycobacterium ulcerans toxin mycolactone. J Exp Med, 204(6):1395-1403.


Lymphoid Tissue development  (Gérard Eberl)


S. Dulauroy, S.E. Di Carlo, F. Langa, G. Eberl and L. Peduto. 2012. Lineage tracing of ADAM12+ perivascular cells reveals a dominant pro-fibrotic pathway during acute injury. Nat. Med., 18:1262-1270.


S. Sawa, M. Lochner, N. Satoh-Takayama, S. Dulauroy, M. Bérard, M. Kleinschek, D. Cua, J.P. Di Santo and G. Eberl. 2011. RORgt+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota. Nat. Immunol., 12:320-326.


S. Sawa, M. Cherrier, M. Lochner, N. Satoh-Takayama, H.J. Fehling, F. Langa, J.P. Di Santo and G. Eberl. 2010. Lineage relationship analysis of RORγt+ innate lymphoid cells. Science, 330:665-669.


G. Eberl. 2010. A new vision of immunity: homeostasis of the superorganism. Mucosal Immunology, 3:450-460. Times cited: 24


D. Bouskra, C. Brézillon, M. Bérard, C. Werts, R. Varona, I. Gomperts Boneca, and G. Eberl. 2008. Lymphoid tissue genesis induced by commensals through NOD1 regulates intestinal homeostasis. Nature, 456:507-510.


Lymphocyte Cell Biology (Andres Alcover)


Das V., Nal B., Dujeancourt A., Thoulouze M. I., Galli T., Roux P., Dautry-Varsat A., Alcover A. Activation-induced polarized recycling targets T cell receptors to the immunological synapse. Involvement of SNARE complexes. Immunity 2004. 20: 577-588.


Thoulouze M. I., Sol-Foulon N., Blanchet F., Dautry-Varsat A., Schwartz O., Alcover A. Human immunodefficiency virus (HIV-1) impairs the formation of the immunological synapse. Immunity. 2006 24 :547-561.


Pais-Correia A. M., Sachse M., Guadagnini S., Robbiati V., Lasserre R., Gessain A., Gout O., Alcover A., Thoulouze M. I. Biofilm-like extracellular viral assemblies mediate HTLV-1 cell-to-cell transmission at virological synapses. Nature Med. 2010. 16: 83-90.


Lasserre, R., Charrin, S., Cuche, C., Danckaert, A., Thoulouze, M. I., de Chaumont, F., Duong, T., Perrault, N., Varin-Blank, N., Olivo-Marin, J. C., Etienne-Manneville, S., Arpin, M., Di Bartolo, V., Alcover, A. Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse. EMBO J. 2010. 29 : 2301-2314.


Lasserre R., Cuche C., Blecher-Gonen R., Libman E, Biquand E., Danckaert A., Yablonski D., Alcover A., and Di Bartolo V. Release of serine/threonine-phosphorylated adaptors from signaling microclusters downregulates T cell activation. J Cell Biol. 2011. 195 : 839-853.


Lymphopoiesis unit (A. Freitas)


Tanchot, C., Lemonnier, F.A., Pérarnau, B., Freitas, A.A. & Rocha, B.

Differential requirements for survival and proliferation of CD8 naïve or memory T cells.

Science 276 : 2057-2062 (1997)


Freitas, A.A. & Rocha, B.

Population biology of lymphocytes: the flight for survival.

Ann. Rev. Immunol. 18 : 83-111 (2000)


Almeida, A.R.M., Legrand, N., Papiernick, M. & Freitas, A.A.

Homeostasis of peripheral CD4+ T cells : IL-2Ra and IL-2 shape a population of regulatory cells that controls CD4+ T cell numbers.

J. Immunol. 169 : 4850-4860 (2002)


Gaudin, E., Hao, Y., Rosado, M.M., Chaby, R., Girard, R. & Freitas, A.A.

Positive selection of B cells expressing low densities of self-reactive BCRs.

J. Exp. Med. 199 : 843-853 (2004)


Montaudouin, C., Anson, M., Hao, Y., Duncker, S.V., Fernandez, T., Gaudin, E., Ehrenstein, M., Kerr, W.G., Colle, J.-H., Bruhns, P., Daëron, M. & Freitas, A.A.

Quorum sensing contributes to activated IgM-secreting B cell homeostasis.

J. Immunol. 190 : 106-114 (2013)


Immune Regulation and Vaccinology (Claude Leclerc)


Sayes, F., Sun, L., Di Luca, M., Simeone, R., Degaiffier, N., Fiette, L., Esin, S., Brosch, R., Bottai, D., Leclerc, C. and Majlessi, L. (2012). Strong Immunogenicity and Cross-Reactivity of Mycobacterium tuberculosis ESX-5 Type VII Secretion-Encoded PE-PPE Proteins Predicts Vaccine Potential. Cell Host Microbe 11, 352-363.


Guillerey, C., Mouries, J., Polo, G., Doyen, N., Law, H.K., Chan, S., Kastner, P., Leclerc, C., and Dadaglio, G. (2012). Pivotal role of plasmacytoid dendritic cells in inflammation and NK cell responses following TLR9 triggering in mice. Blood 120, 90-99


Freire, T., Zhang, X., Dériaud, E., Ganneau, C., Vichier-Guerre, S., Azria, E., Launay, O., Lo-Man, R., Bay, S., and Leclerc, C. (2010). Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant. Blood 116, 3526-36.


Zhang, X. Majlessi, L., Deriaud, E., Leclerc, C., and Lo-Man, R. (2009). Coactivation of Syk kinase and MyD88 adaptor protein pathways by bacteria promotes regulatory properties of neutrophils. Immunity 31, 761-71.


Mouries, J., Moron, G., Schlecht, G., Escriou, N., Dadaglio, G., and Leclerc, C. (2008). Plasmacytoid dendritic cells efficiently cross-prime naive T cells in vivo after TLR activation. Blood 112, 3713-3722


Cytokines signalisation (Sandra Pellegrini)


Moraga, M., D. Harari, G. Schreiber, G. Uzé, and S. Pellegrini. 2009 Receptor density is key to the interferon alpha2/beta differential activities. Mol. Cell. Biol. 129:4778-4787


Dong S., B. Corre, K. Nika, S. Pellegrini, and F. Michel. 2010. T cell receptor signal initiation induced by low-grade stimulation requires the cooperation of LAT in human T cells. PLoS ONE. 5(11):e15114.


Francois-Newton, V., G. de Freitas Almeida, B. Payelle-Brogard, D. Monneron, L. Pichard-Garcia, J. Piehler, S. Pellegrini, and G. Uzé . 2011. USP18-based negative feed-back control is induced by Type I and Type III Interferons and specifically inactivates interferons a response. PLoS One. 6:e22200.


Francois-Newton V, Livingstone M, Payelle-Brogard B, Uzé G, Pellegrini S. 2012. USP18 establishes the transcriptional and anti-proliferative interferon α/β differential. Biochem J. 446:509-16


Li Z, Gakovic M, Ragimbeau J, Eloranta ML, Rönnblom L, Michel F, Pellegrini S. 2013.

Two rare disease-associated Tyk2 variants are catalytically impaired but signaling competent.

J Immunol. 190, 2335-44


Innate Immunity (James Di Santo)


Vosshenrich, C.A.J., García-Ojeda, M.E., Samson-Villeger, S.I., Pasquelleto, V., Enault, L., Richard-Le Goff, O., Corcuff, E., Guy-Grand, D., Rocha, B., Cumano A., Rogge, L., Ezine, S. and Di Santo, J.P. (2006) A thymic pathway of murine natural killer cell development characterized by Gata3 and interleukin-7 receptor expression. Nature Immunology 7:1217-1224.


Satoh-Takayama, N., Vosshenrich, C.A.J., Lesjean-Pottier, S.., Sawa, S., Lochner, M., Rattis, F., Mention, J.-J., Thiam, K., Cerf-Bensussan, N., Mandelboim, O., Eberl, G. and Di Santo, J.P. (2008) Microbial flora drives IL-22 production in intestinal NKp46+ cells that provide innate mucosal immune defense. Immunity 29:958-970.


Huntington, N.D., Legrand, N., Alves, N.L., Jaron, B., Weijer, K., Plet, A., Corcuff, E., Mortier, E., Jacques, Y., Spits, H. and Di Santo, J.P. (2009) IL-15 trans-presentation promotes human NK cell development and terminal differentiation in vivo. Journal of Experimental Medicine 206:25-34.


Yusa, K., Rashid, S.T., Strick-Marchand, H., Miranda, E., Ordonez, A., Hannan, N., Rohani, F., Alexander, G., Marciniak, S.J., Gregory, P.D., Di Santo, J.P., Lomas, D.A., Bradley, A. and Vallier, L. (2011) Efficient bi-allelic correction of a1-antitrypsin deficiency in human induced pluripotent stem cells. Nature 478:391-394.


García-Ojeda, M.E., Klein Wolterink, R.G.J., Lemaitre, F., Richard-Le Goff, O., Hasan, M., Hendriks, R.W., Cumano, A. and Di Santo, J.P. (2013) GATA-3 promotes T cell specification by repressing B cell potential in pro-T cells in mice. Blood 121:1749-1759.


Laboratory of Antibodies in Therapy & Pathology (Pierre Bruhns)


Guilliams M, Bruhns P, Saeys Y, Hammad H and Lambrecht B. The function of Fcγ receptors in dendritic cells and macrophages. Nat Rev Immunol, 2014; 14, 94-108.


Albanesi M, Mancardi DA, Jönsson F, Iannascoli B, Fiette L, Di Santo JP, Lowell CA and Bruhns P. Neutrophils mediate antibody-induced anti-tumor effects in mice. Blood, 2013; 122, 3160-3164


Mancardi DA, Albanesi M, Jönsson F, Iannascoli B, Van Rooijen N, Kang X, England P, Daëron M and Bruhns P. The high-affinity human IgG receptor FcγRI (CD64) promotes IgG-mediated inflammation, anaphylaxis and anti-tumor immunotherapy. Blood, 2013; 121(9): 1563-1573.


Bruhns P. Properties of mouse and human IgG receptors and their contribution to disease models. Blood, 2012; 119(24):5640-5649.


Jönsson F, Mancardi DA, Zhao W, Kita Y, Iannascoli B, Khun H, Van Rooijen N, Shimizu T, Schwartz LB, Daëron M and Bruhns P. Human FcγRIIA induces anaphylactic and allergic reactions. Blood, 2012; 119(11):2533-44.



Immunoregulation Research unit (Lars Rogge)


Combinatorial control of Th17 and Th1 cell function by genetic variation at genes associated with the IL-23 signaling pathway in spondyloarthritis.

Coffre M, Roumier M, Rybczynska M, Sechet E, Law HK, Gossec L, Dougados M, Bianchi E, Rogge L.

Arthritis Rheum. 2013 Mar 18. doi: 10.1002/art.37936. [Epub ahead of print]. PMID: 23508476


In vivo expansion of naive and activated CD4+CD25+FOXP3+ regulatory T cell populations in interleukin-2-treated HIV patients.

Weiss L, Letimier FA, Carriere M, Maiella S, Donkova-Petrini V, Targat B, Benecke A, Rogge L*, Levy Y*.

Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10632-7. PMID: 20498045 [* equal contribution]


Integration of distinct intracellular signaling pathways at distal regulatory elements directs T-bet expression in human CD4+ T cells.

Placek K, Gasparian S, Coffre M, Maiella S, Sechet E, Bianchi E, Rogge L.

J Immunol. 2009 Dec 15;183(12):7743-51. PMID: 19923468


Chromatin remodeling by the SWI/SNF-like BAF complex and STAT4 activation synergistically induce IL-12Rbeta2 expression during human Th1 cell differentiation.

Letimier FA, Passini N, Gasparian S, Bianchi E, Rogge L.

EMBO J. 2007 Mar 7;26(5):1292-302. PMID: 17304212


Transcript imaging of the development of human T helper cells using oligonucleotide arrays.

Rogge L, Bianchi E, Biffi M, Bono E, Chang SY, Alexander H, Santini C, Ferrari G, Sinigaglia L, Seiler M, Neeb M, Mous J, Sinigaglia F, Certa U.

Nat Genet. 2000 May;25(1):96-101. PMID: 10802665






L. Deriano and D.B. Roth. Modernizing the Nonhomologous End Joining Repertoire: Alternative and Classical NHEJ Share The Stage. Annual Review of Genetics. 2013 Nov 23; 47:433-55.


J. Chaumeil, M. Micsinai, P. Ntziachristos, D.B. Roth, I. Aifantis, Y. Kluger, L. Deriano, J.A. Skok. The C-terminus of RAG2 and ATM control RAG activity to limit the number of potential translocation substrates. Nature Communications. 2013 Jul 31; 4:2231.


J. Chaumeil, M. Micsinai, P. Ntziachristos, L. Deriano, J. M. Wang, Y. Ji, E.P. Nora, M.J. Rodesch, J.A. Jeddeloh, I. Aifantis, Y. Kluger, D.G. Schatz, J.A. Skok. Higher-order looping and nuclear organization of antigen receptor loci facilitate targeted RAG cleavage and regulated rearrangement in recombination centers. Cell Reports. 2013 Feb 21; 3(2):359-70.


M. Mijušković, S.M. Brown, Z. Tang, C.R. Lindsay, E. Efstathiadis, L. Deriano, D.B. Roth. A streamlined method for detecting structural variants in cancer genomes by short read paired-end sequencing. PLoS One. 2012 Oct 29; 7(10):e48314.


L. Deriano, J. Chaumeil, M. Coussens, A. Multani, Y. Chou, A.V. Alekseyenko, S. Chang, J.A. Skok, D.B. Roth. The RAG2 C-terminus suppresses genomic instability and lymphomagenesis. Nature. 2011 Mar 3; 471(7336): 119-2.


L. Deriano, T.H. Stracker, A. Baker, J.H.J. Petrini, D.B. Roth. Roles for NBS1 in alternative end joining of V(D)J recombination intermediates. Mol Cell. 2009 Apr 10; 34(1): 13-25.


B. Corneo*, R.L. Wendland*, L. Deriano*, X. Cui, I.A. Klein, S. Wong, S. Arnal, A.J. Holub, G.R. Weller, B.A. Pancake, S. Shah, V.L. Brandt, K. Meek, D.B. Roth. RAG mutations reveal robust alternative end joining. Nature, 2007 Sep; 449(7161): 483-6. (*Equal contribution)


Updated on 26/02/2014