Mouse Genetics Engineering Center

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Francina Langa Vives
francina@pasteur.fr

 

CIGM

Officially created in June 2003, the Centre d’Ingénierie Génétique Murine (CIGM) is a novel technological facility at the Institut Pasteur. The CIGM is open to the Pasteur scientific community (and also to external customers) interested in new transgenic, knock-out or knock-in murine models.

The CIGM aims to generate genetically-engineered mice either by:  

 

Classical transgenesis techniques, consisting of microinjection of the gene of interest (ADN fragment, BAC or YAC) into the pronuclei of fertilized eggs.

 

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The service is available in hybrid (B6SJLF1, B6CBAF1) and pure (C57BL6/N, FVB/N) genetic backgrounds.
 

 

Targeted mutation by homologous recombination in ES cells and germline transmission.

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Chimera crossing for the obtention of heterozygous mice for the desired mutation.

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