The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his ...
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources ...
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over ...
The mission of the Industrial Partnership team is to detect, promote, assist and protect the inventive activities from research (inventions, know-how and biological materials) conducted at the Institut Pasteur (and in some Institutes of its international network), and transfer there to industrial ...
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study ...
New signaling pathways involved in the Listeria entry into target cells
This project is realized in collaboration with To Nam Tham (Engineer). In order to better understand the signaling cascades triggered by Listeria during entry into target cells, we have performed a proteomic and functional characterization of phagosomes containing latex beads coated with the Listeria invasion molecules InlA and InlB. A functional assay to investigate lipid kinases has led us to discover the presence of type II phosphatidylinositol 4-kinases (PI4-K) at the membrane of InlB-derived phagosomes. These kinases participate in the production of a pool of phosphatidylinositol-4-phosphate (PI4P) that would not be involved in the formation of phosphatidylinositol-3,4,5-triphosphate (PI3,4,5P3) –which is an upstream effector of Rac for cytoskeletal rearrangements during Listeria entry. Instead, this PI4P pool would be required for the recruitment of still unidentified effectors in a different signaling pathway that are also required for entry. We are investigating at this stage which are this downstream effectors of the type II PI 4-Ks, as well as the molecular events required for their recruitment at the bacterial entry site downstream of the cellular receptor for InlB, the hepatocyte growth factor receptor of Met.
The proteomic characterization of phagosomes containing InlA- and InlB-derived phagosomes identified the presence of several unexpected proteins in the Listeria internalization pathways including the small GTPases Rab10 septin 9, and we are also investigating their participation in the infection process.
Updated on 12/05/2014
Unité Interactions Bactéries-Cellules
INSERM U604 INRA USC2020
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE
Phone: + 33 (1) 45 68 88 41
Secretary: + 33 (1) 40 61 30 32
Fax: + 33 (1) 45 68 87 06
Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)
The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.
The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.