The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his wife and can admire his rich and diverse scientific work.
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources needed to speed up and further improve the quality of their outstanding research.
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over the world who come to further their training or top up their degree programs.
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study and traineeship fellowships are available to scientists. Alongside training, dynamism and attractiveness of RIIP will result in the creation of 4-year group for the young researchers.
The RickA Protein of Rickettsia conorii is a novel activator of Arp2/3 complex
Vérinique Villiers and Edith Gouin work together on the Rickettsia project. Listeria monocytogenes, Shigella flexneri, and Rickettisia conorii are three bacterial pathogens that are able to polymerize actin into ’comet tail’ structures and move within the cytosol of infected cells. The actin-based motilities of L. monocytogenes and S. flexneri are known to require the bacterial proteins ActA and IscA, respectively, and several mammalian cytoskeleton proteins including Arp2/3 complex and VASP (vasodilator-stimulated phosphoprotein) for L. monotyogenes and vinculin and N-WASP (the neural Wiskott-Aldrich syndrome protein) for S. flexneri.
We have previously reported that the actin tails of Rickettsia conorii, another intracellular bacterium, unlike those of Listeria of Shigella are composed of long unbranched actin filaments apparently devoid of Arp2/3 complex (J. Cell. Science, 1999).
Then, we identified a R. conorii surface protein, RickA, as a novel type of Arp2/3 activator. In vitro, RickA activates, albeit far less efficiently than ActA, the nucleating activity of Arp2/3 and its capacity to induce branch formation. In infected cells, Arp2/3 is detected on the bacterial surface but not in the Rickettsia tails, as it is the case for Listeria and Shigella tails. When expressed in mammalian cells, and targeted to the membrane, RickA induces filopodia. Thus RickA-induced actin polymerisation, which results in the generation of the long actin filaments reminiscent of those present in filopodia, appears as a powerful tool for the study of filopodia formation.
The Rickettsia actin tails consist of long filaments. Electron microscopy of myosin S1 decorated actin tails of R. conorii and L. monocytogenes.
Updated on 12/05/2014
Unité Interactions Bactéries-Cellules
INSERM U604 INRA USC2020
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE
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Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)
The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.
The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.