Curriculum vitae Edith Gouin

Edith GOUIN

Professional address :
INSTITUT PASTEUR
Bacteria-cell interactions Unit – INSERM U604 – INRA USC2020
Department of Cell biology and Infection
25 rue du Docteur Roux -75724 PARIS Cedex 15-France.
E-mail: edithgouin.png


Present position and status: Staff scientist, Engineer

 

UNIVERSITARY EDUCATION
1990     Diploma of Bacteriology, Institut Pasteur, Paris, France.
1985     Master of Cellular and Molecular Biology (DEA), University of Paris VII, France.
1978     Degree in Biochemistry (Maitrise), University of Paris XI, France.
1977     Licence  of Biochemistry, University of Orléans, France.
1976     Degree of Technician in Biochemistry, Paris, France.
1974     Certificate of Parasitology, University of Medicine, Tours, France.

 

RESEARCH EXPERIENCE
2000-Present     Research Engineer, Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France. "Studies of the actin-based motility of Rickettsia conorii".
1994-2000          Laboratory Technician, Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France. "Studies of the actin-based motility of Rickettsia conorii".
1991-1993          Laboratory Technician, Laboratoire de Génétique Moléculaire des Listeria, Institut Pasteur, Paris, France. "Study of the mechanism used by the protein ActA in the actin-dependant motility of Listeria".
1990-1991          Laboratory Technician, Unité de Génie Microbiologique, Institut Pasteur Paris, France. "Identification of the Internalin family in Listeria".
1988-1989          Laboratory Technician, Unité de Virologie Médicale et Vaccins Viraux, Institut Pasteur, France. "Development of a cold probe for CMV detection".
1980-1985          Laboratory Technician, Unité de Radioimmunologie Analytique. "Pharmacologic studies of Prostaglandin A1 in the process of tumor cells in vitro".

 

TEACHING
Practical courses
2003-2005    Microbiology Course, Institut Pasteur, Paris, France.
2005-2003-2002    Molecular Biology of the Cell Course, Institut Pasteur, Paris, France.
1995    INSERM Workshops, Institut Pasteur, Paris, France. "Molecular and cellular tools for microbial pathogeny studies"
1990    Microbiology Course, Institut Pasteur, Paris, France.
1981-1985    Immunology Course, Institut Pasteur, Paris, France.

 

PUBLICATION LIST
1. Raichvarg, D., Marchand, E., Sarfati, G. and Agneray, J. (1980). Technique colorimétrique d’évaluation de l’activité phagocytaire des macrophages péritonéaux de souris. Ann. Immunol. Institut Pasteur.131 D, 71-78.
2. Fafeur, V., Gouin, E. and Dray, F. (1985). Growth hormone-releasing factor (GRF) stimulates PGE2 production in rat anterior pituitary. Evidence for a PGE2 involvement in GRF-induced GH release. Biochem. Biophys. Res. Commun., 126, 725-733.
3. Gouin, E., Vulliez-Le Normand, B., Gouyette, A., Heidet, V., Nagel, N. and Dray, F. (1986). Tumor cell biotransformation products of prostaglandin A1 with growth inhibitory activity. Biochem. Biophys. Res. Commun., 141, 1254-1260.
4. Dray, F., Gouin, E., and Rougeot, C.. (1986). Adrenocorticotropic Hormone, ACTH. Methods of Enzymatic Analysis. Vol.IX, 348-362.
5. Gaillard, J.L., Berche, P., Frehel, C., Gouin, E. and Cossart, P.. (1991). Entry of L. monocytogenes  into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from Gram-positive cocci. Cell, 65, 1127-1141.
6. Mengaud, J., Dramsi, S., Gouin, E., Vazquez-Boland, J.A., Milon, G. and Cossart, P. (1991). Pleiotropic control of Listeria monocytogenes  virulence factors by a gene that is autoregulated. Mol. Microbiol., 59, 2273-2283.
7. Kocks, C., Gouin, E., Tabouret, M.,  Berche, P., Ohayon, H. and Cossart, P. (1992). L. monocytogenes  induced actin assembly requires the actA  gene product, a surface protein. Cell, 68, 521-531.
8. Dramsi, S., Gouin, E., Vasquez-Boland, J.A., Geoffroy, C., Mengaud, J., and Cossart, P.. (1992). Listeria monocytogenes : Coexpression of listeriolysin O with other virulence factors. In Bacterial Protein Toxins. 472-477, B. Witholt, J.E. Alouf, G.J. Boulnois, P. Cossart, B.W. Dijstra, P. Falmagne, F.J. Fehrenbach, J. Freer, H. Niemann, R. Rappuoli and T. Wadstrom, eds Gustav Fisher Verlag. Stuttgart. Jena. New York.
9. Gouin, E., Mengaud, J. and Cossart, P. (1994). The virulence gene cluster of Listeria monocytogenes  is also present in Listeria ivanovii, an animal pathogen and Listeria seeligeri, a non pathogenic species. Infect. Immun., 62, 3550-3553.
10. Sheehan, B., Kocks, C., Dramsi, S., Gouin, E., Klarsfeld, A., Mengaud, J., and Cossart, P.. (1994). Molecular and Genetic Determinants of Listeria monocytogenes pathogenesis. Current Topics in Microbiology and Immunology, 192, 187-216.
11. Gouin, E., Dehoux, P., Mengaud, J., Kocks, C. and Cossart, P. (1995). iactA of Listeria ivanovii although distantly related to Listeria monocytogenes actA restores actin tail formation in a L. monocytogenes actA mutant. Infect. Immun., 63, 2729-2737.
12. Friedrich, E., Gouin, E., Hellio, R., Kocks, C., Cossart, P. and Louvard, D. (1995). Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function. EMBO J., 14, 2731-2744.
13. Kocks, C., Marchand, J.B., Gouin, E., d’Hauteville, H., Sansonetti, P.J., Carlier, M.F. and Cossart, P. (1995). The unrelated surface proteins ActA of Listeria monocytogenes  and icsA of Shigella flexneri are sufficient to confer actin-based motility on Listeria innocua and Escherichia coli respectively. Mol. Microbiol., 18, 413-423.
14. Lasa, I., David, V., Gouin, E., Marchand, J.B. and Cossart, P. (1995). The amino-terminal part of ActA is critical for the actin-based motility of Listeria monocytogenes ; the central proline-rich acts as a stimulator. Mol. Microbiol., 18, 425-436.
15. Lasa, I., Gouin, E., Goethals, M., Vancompernolle, K., David, V., Vandekerckhove, J. and Cossart, P. (1997). Identification of two regions in the amino-terminal domain of ActA involved in the actin comet tail formation by Listeria monocytogenes . EMBO J., 16, 1531-1540
16. Mourrain,P., Lasa, I., Gautreau, A., Gouin, E., Pugsley, A. and Cossart, P. (1997). ActA is a dimer. Proc. Natl. Acad. Sci. USA., 94, 10034-10039.
17. Robichon, D., Gouin, E., Debarbouillé, M., Cossart, P., Cenatiempo, Y. and Héchard, Y. (1997). The rpoN (_54) gene from Listeria monocytogenes is involved in resistance to mesentericin Y105, an antibacterial peptide from Leuconostoc mesenteroides. J. Bacteriol., 179, 7591-7594
18. David, V., Gouin, E., Van Troys, M., Grogan, A., Segal, W., Ampe, C. and Cossart, P.. (1998). Identification of cofilin, coronin, Rac and capZ in  actin tails using a Listeria affinity approach. J. Cell Science, 111, 2877-2884.
19. Gouin, E., Gantelet, H., Egile, C., Lasa, I., Ohayon, H., Villiers, V., Gounon, P., Sansonetti, P.J. and Cossart, P. (1999).  A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J. Cell Sci., 112, 1697-1708.
20. Dalet, K., Gouin, E., Cenatiempo, Y., Cossart, P. and Hechard, Y. (1999). Characterisation of a new operon encoding a Zur-kike protein and an associated ABC zinc permease in Listeria monocytogenes. FEMS Microbiol. Lett, 174, 111-116.
21. Castellano, F., Montcourrier, P., Guillemot, J.C, Gouin, E., Machewsky, L., Cossart, P. and Chavrier, P. (1999). Inducible recruitment of Cdc42 or WASP to a cell-surface receptor triggers actin polymerization and filopodium formation. Curr. Biol., 9, 351-360.
22. Steffen, P., Schafer, D. A., David, V., Gouin, E., Cooper, J. A. and Cossart, P. (2000). The Listeria monocytogenes ActA protein interacts with phosphatidyl-inositol 4,5 biphosphate in vitro. Cell motil. Cytoskeleton, 45, 58-66.
23. Gouin, E., Boujemaa-Paterski, R., Hansen, G., Samarin, S., Le Clainche, C., Didry, D., Dehoux, P., Cossart, P., Kocks, C., Carlier, M.F. and Pantaloni, D. (2001). Listeria protein ActA mimics WASp family proteins: it activates filament barbed end branching by Arp2/3 complex. Biochemistry, 40,11390-404.
24. Bierne, H., Gouin, E., Roux, P., Caroni, P., Yin, H.L. and Cossart, P. (2001) A role for cofilin and LIM kinase in Listeria-induced phagocytosis. J. Cell Biol., 155, 101-12.
25. Jacquet, C., Gouin, E., Jeannel, D., Cossart, P. and Rocourt, J. (2002). Expression of ActA, Ami, InlB, and listeriolysin O in Listeria monocytogenes of human and food origin. Appl. Environ. Microbiol., 68, 616-22.
26. Renesto, P., Gouin, E., Raoult, D., (2002). Expression of green fluorescent protein in Rickettsia conorii. Microb. Pathog., 33, 17-31.
27. Pizarro-Cerda, J., Jonquieres, R., Gouin, E., Vandekerckhove, J., Garin, J. and Cossart, P. (2002). Distinct protein patterns associated with Listeria monocytogenes InlA- or InlB-phagosomes. Cell. Microbiol., 4, 101-15.
28. Renesto, P., Dehoux, P., Gouin, E., Touqui, L., Cossart, P. and Raoult, D. (2003). Identification and characterization of a phospholipase D-superfamily gene in Rickettsiae. J. Infect. Dis., 188, 1276-83.
29. Gouin, E., Egile, C., Dehoux, P., Villiers, V., Adams, J., Gertler, F., Li, R. and Cossart, P. (2004). The RickA protein of Rickettsia conorii activates the Arp2/3 complex. Nature, 427, 457-61.
30. Archambaud, C., Gouin, E., Pizarro-Cerda, J., Cossart, P., Dussurget, O. (2005). Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence of Listeria monocytogenes. Mol. Microbiol., 56, 383-96.
31. Gouin, E., Welch, W.E., and Cossart, P. (2005). Actin-based motility of intracellular pathogens. Current Opinion in Microbiology, 8, 35-45.
32. Sousa S., Cabanes D., Archambaud C., Colland F., Lemichez E., Popoff M., Boisson-Dupuis S., Gouin E., Lecuit M., Legrain P., Cossart P. (2005) ARHGAP10 is necessary for alpha-catenin recruitment at adherens junctions and for Listeria invasion. Nat Cell Biol. 10, 954-60.
33. Herro R, Poncet S, Cossart P, Buchrieser C, Gouin E, Glaser P, Deutscher J. How (2005) Seryl-phosphorylated HPr inhibits PrfA, a transcription activator of Listeria monocytogenes virulence genes. J Mol Microbiol Biotechnol. 9, 224-34.
34. Birmingham C. L., Canadien V., Gouin E., Troy E.B., Yoshimori T., Cossart P., Higgins D., Brumell J.H. (2007) Listeria monocytogenes Evades Killing by Autophagy During Colonization of Host Cells. Autophagy. 3, 442-51.
35. Sabet C, Toledo-Arana A, Personnic N, Lecuit M, Dubrac S, Poupel O, Gouin E, Nahori MA, Cossart P, Bierne H. (2008). The Listeria monocytogenes virulence factor InlJ is specifically expressed in vivo and behaves as an adhesin. Infect Immun. 76, 1368-78.

Updated on 12/05/2014

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Lab address

Unité Interactions Bactéries-Cellules

INSERM U604 INRA USC2020
Institut Pasteur
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE

 

Phone: + 33 (1) 45 68 88 41

Secretary: + 33 (1) 40 61 30 32

Fax: + 33 (1) 45 68 87 06

 

Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)

The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.

The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.

For more information: Pasteur Institute Access and Paris Metro/Bus/Tram

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