Physiopathology of the Infection and Host Immune Responses to Listeria monocytogenes



[Cossart, PNAS 2011]

The infection by L. monocytogenes in vivo: bacteria, via contaminated food product, reach the intestinal barrier, cross it, and then disseminate to the brain and placenta.





[Lecuit, TRENDS in Molecular Medicine 2002]

Model for the pathophysiology of Listeria infection




[Photo: O. Dussurget, unité Interactions Bactéries-Cellules, Institut Pasteur]

Bioluminescence imaging of murine listeriosis. Luminescent Listeria monocytogenes at 5X104 CFU per BALB/c mouse were imaged 4 days post-intravenous infection with an IVIS100 imaging system (Xenogen Corp.). One mouse displayed a characteristic signal of brain growth while the four other animals controlled the infection.






[Lecuit, TRENDS in Molecular Medicine 2002]

E-cadherin expression in mice. a) Enterocyte-restricted human E-cadherin expression in iFABP-hEcad [human E-cadherin under the control of the promoter of the intestinal fatty-acid-binding protein (iFABP) gene] transgenic mice. Small intestine sections of a iFABP-hEcad transgenic mouse (left) and a non-transgenic littermate (right) immunolabeled with anti-hEcad HECD1 mouse monoclonal antibody (red). HECD1 recognizes human but not mouse E-cadherin. The background signal in the lamina propria is because of the reactivity of the secondary goat anti-mouse IgG antibody towards endogenous mouse IgG. A cross-section of a transgenic intestinal epithelial sheet allows detection of the typical honeycomb-like pattern of E-cadherin labeling. Scale BAR = 10 μm. b) Listeria monocytogenes multiplication within the lamina propria of a small-intestinal villus of an iFABP-hEcad transgenic mouse 48 h after intragastric inoculation. A small intestine section of a iFABP-hEcad transgenic mouse orally infected with L. monocytogenes and immunolabeled with rabbit anti-L. monocytogenes R11 polyclonal antibody (red). L. monocytogenes have crossed the intestinal barrier and replicate in the lamina propria, a phenomenon that is never observed in non-transgenic mice. Scale BAR = 10 μm.


Updated on 13/05/2014



Lab address

Unité Interactions Bactéries-Cellules

Institut Pasteur
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE


Phone: + 33 (1) 45 68 88 41

Secretary: + 33 (1) 40 61 30 32

Fax: + 33 (1) 45 68 87 06


Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)

The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.

The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.

For more information: Pasteur Institute Access and Paris Metro/Bus/Tram