Physiopathology of the Infection and Host Immune Responses to Listeria monocytogenes

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[Cossart, PNAS 2011]

The infection by L. monocytogenes in vivo: bacteria, via contaminated food product, reach the intestinal barrier, cross it, and then disseminate to the brain and placenta.

 

 

 

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[Lecuit, TRENDS in Molecular Medicine 2002]

Model for the pathophysiology of Listeria infection

 

 

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[Photo: O. Dussurget, unité Interactions Bactéries-Cellules, Institut Pasteur]

Bioluminescence imaging of murine listeriosis. Luminescent Listeria monocytogenes at 5X104 CFU per BALB/c mouse were imaged 4 days post-intravenous infection with an IVIS100 imaging system (Xenogen Corp.). One mouse displayed a characteristic signal of brain growth while the four other animals controlled the infection.

 

 

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[Lecuit, TRENDS in Molecular Medicine 2002]

E-cadherin expression in mice. a) Enterocyte-restricted human E-cadherin expression in iFABP-hEcad [human E-cadherin under the control of the promoter of the intestinal fatty-acid-binding protein (iFABP) gene] transgenic mice. Small intestine sections of a iFABP-hEcad transgenic mouse (left) and a non-transgenic littermate (right) immunolabeled with anti-hEcad HECD1 mouse monoclonal antibody (red). HECD1 recognizes human but not mouse E-cadherin. The background signal in the lamina propria is because of the reactivity of the secondary goat anti-mouse IgG antibody towards endogenous mouse IgG. A cross-section of a transgenic intestinal epithelial sheet allows detection of the typical honeycomb-like pattern of E-cadherin labeling. Scale BAR = 10 μm. b) Listeria monocytogenes multiplication within the lamina propria of a small-intestinal villus of an iFABP-hEcad transgenic mouse 48 h after intragastric inoculation. A small intestine section of a iFABP-hEcad transgenic mouse orally infected with L. monocytogenes and immunolabeled with rabbit anti-L. monocytogenes R11 polyclonal antibody (red). L. monocytogenes have crossed the intestinal barrier and replicate in the lamina propria, a phenomenon that is never observed in non-transgenic mice. Scale BAR = 10 μm.

 

Updated on 13/05/2014

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Unité Interactions Bactéries-Cellules

INSERM U604 INRA USC2020
Institut Pasteur
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE

 

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