Phosphoinositide Metabolism

 

 

 

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[Kuhbacher et al., The Journal of Biological Chemistry 2012]

Actin polymerization precedes OCRL recruitment to L. monocytogenes vacuoles. Microscopy analysis of the distribution of endogenous OCRL, EGFP-OCRL-a, and actin in cells infected by L. monocytogenes P14.PrfA*. Bar: 10_m(inset bar: 1_m).

 

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[Pizarro-Cerda et al., Nature Cell Biology 2004]

Main steps in phosphoinositide synthesis and degradation, showing some of the enzymes that catalyse the reactions and the steps subverted by bacterial pathogens. Yersinia species induce activation of a PIP(5)K to promote PtdIns(4,5)P2 formation; Listeria monocytogenes and UPEC promote the activity of the class I PI(3)K to generate PtdIns(3,4,5)P3, whereas EPEC blocks its activity; Mycobacterium tuberculosis, blocks a class III PI(3)K involved in the formation of PtdIns(3)P; Shigella flexneri translocates into host cells the bacterial enzyme IpgD that dephosphorylates PtdIns(4,5)P2 into PtdIns(5)P; Salmonella enterica also translocates a bacterial effector, SigD/SopB, that has phosphatidylinositol polyphosphate phosphatase activity and it has been proposed to dephosphorylate the 5'-phosphate from PtdIns(3,5)P2 and PtdIns(3,4,5)P3; SigD/SopB is also implicated in the turnover of PtdIns(4,5)P2, but the precise PtdIns(4,5)P2 hydrolysis site remains to be determined. SigD/SopB additionally has inositol phosphate phosphatase activity (not shown in the diagram).

Updated on 13/05/2014

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Lab address

Unité Interactions Bactéries-Cellules

INSERM U604 INRA USC2020
Institut Pasteur
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE

 

Phone: + 33 (1) 45 68 88 41

Secretary: + 33 (1) 40 61 30 32

Fax: + 33 (1) 45 68 87 06

 

Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)

The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.

The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.

For more information: Pasteur Institute Access and Paris Metro/Bus/Tram

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