Phosphoinositide Metabolism






[Kuhbacher et al., The Journal of Biological Chemistry 2012]

Actin polymerization precedes OCRL recruitment to L. monocytogenes vacuoles. Microscopy analysis of the distribution of endogenous OCRL, EGFP-OCRL-a, and actin in cells infected by L. monocytogenes P14.PrfA*. Bar: 10_m(inset bar: 1_m).




[Pizarro-Cerda et al., Nature Cell Biology 2004]

Main steps in phosphoinositide synthesis and degradation, showing some of the enzymes that catalyse the reactions and the steps subverted by bacterial pathogens. Yersinia species induce activation of a PIP(5)K to promote PtdIns(4,5)P2 formation; Listeria monocytogenes and UPEC promote the activity of the class I PI(3)K to generate PtdIns(3,4,5)P3, whereas EPEC blocks its activity; Mycobacterium tuberculosis, blocks a class III PI(3)K involved in the formation of PtdIns(3)P; Shigella flexneri translocates into host cells the bacterial enzyme IpgD that dephosphorylates PtdIns(4,5)P2 into PtdIns(5)P; Salmonella enterica also translocates a bacterial effector, SigD/SopB, that has phosphatidylinositol polyphosphate phosphatase activity and it has been proposed to dephosphorylate the 5'-phosphate from PtdIns(3,5)P2 and PtdIns(3,4,5)P3; SigD/SopB is also implicated in the turnover of PtdIns(4,5)P2, but the precise PtdIns(4,5)P2 hydrolysis site remains to be determined. SigD/SopB additionally has inositol phosphate phosphatase activity (not shown in the diagram).

Updated on 13/05/2014



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Unité Interactions Bactéries-Cellules

Institut Pasteur
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE


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